RESUMO
A structure-determined silver nanocluster of [Ag10 (Eth)4 (CF3 COO)6 (CH3 OH)3 ]·3C-H3 OH (Eth = ethisterone) (1), is firstly demonstrated by self-assembly of silver salt and ethisterone. Due to the thiophilicity of silver(I) ions, complex 1 shows reactivity with glutathione (GSH) molecules in solution and induces the fluorescence quenching behavior. Thus, complex 1 can be used as a fluorescent sensor for GSH. In consideration of the higher level of GSH in cancerous cells, complex 1 presents significant tumor suppression reactivity toward the human hepatocellular carcinoma (HepG2) cells with IC50 value of 165 × 10-9 m. Especially, complex 1 displays 3.4-fold higher in vitro cytotoxicity to HepG2 cells than that of the normal CCC-HEL-1 cells, which makes complex 1 a potential targeting suppression agent for cancerous cells. The molecular design of complex 1 not only generates a new medicine-silver(I) cluster family, but also opens a new avenue to the targeting anticancer organosilver(I) materials.
Assuntos
Estrogênios/farmacologia , Glutationa/metabolismo , Nanopartículas/química , Prata/farmacologia , Antineoplásicos/farmacologia , Morte Celular/efeitos dos fármacos , Forma Celular/efeitos dos fármacos , Etisterona/farmacologia , Células Hep G2 , Humanos , Nanopartículas/ultraestrutura , Tamanho da Partícula , Espectrometria de FluorescênciaRESUMO
7 compounds were isolated from the ethyl-acetate extract of Vaccinium iteophyllum Hance by using repeated silical gel column chromatography. These 7 compounds were identified by means of physico-chemical propertic and spectroscopic analysis as beta-sitosterol (I), ursolic acid (II), taraxerol (III), taraxerone (IV), friedelin (V), friedelinol (VI), 19,24-dihydroxyurs-12-en-3-one-28-oic acid (VII). The chemical constituents from this plant were reported here for the first time.
Assuntos
Plantas Medicinais/química , Vaccinium/química , Acetatos , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Ácido Oleanólico/análogos & derivados , Ácido Oleanólico/química , Ácido Oleanólico/isolamento & purificação , Folhas de Planta/química , Caules de Planta/química , Sitosteroides/química , Sitosteroides/isolamento & purificação , Triterpenos/química , Triterpenos/isolamento & purificaçãoRESUMO
To explore the same and difference of chemical components between the chromatographic fingerprints of volatile oil of Rhizoma Curcumae Longae and Rhizoma Wenyujin Concisum, the chromatographic/spectroscopic data of fingerprints were comparative analyzed using modified window target-testing factor analysis method. The results of comparisons showed that ten chemical components present in Rhizoma Curcumae Longae are absent in Rhizoma Wenyujin Concisum, while nine chemical components are present in Rhizoma Wenyujin Concisum but absent in Rhizoma Curcumae Longae. Also, nine common components are both present in Rhizoma Curcumae Longae and Rhizoma Wenyujin Concisum.
