Comprehensive characterization and expression analysis of enzymatic antioxidant gene families in passion fruit (Passiflora edulis).

Passion fruit, a valuable tropical fruit, faces climate-related growth challenges. Antioxidant enzymes are vital for both stress protection and growth regulation in plants. We first provided systemic analysis of enzymatic antioxidant gene families in passion fruit, identifying 90 members including 11 PeSODs, 45 PeAPXs, 8 PeCATs, 7 PeGPXs, 6 PeMDHARs, 8 PeDHARs, and 5 PeGRs. Gene members in each gene family with same subcellular localization showed closer phylogenetic relationship. Many antioxidant genes exhibited tissue- or developmental stage-specific expression patterns during floral and fruit development, with some widely expressed. Their co-expressed genes were linked to photosynthesis and energy metabolism, suggesting roles in protecting highly proliferating tissues from oxidative damage. Potential genes for enhancing temperature stress resistance were identified. The involvement of diverse regulatory factors including miRNAs, transcription factors, and CREs might contribute to the complex roles of antioxidant genes. This study informs future research on antioxidant genes and passion fruit breeding.

Revealing Further Insights into Astringent Seeds of Chinese Fir by Integrated Metabolomic and Lipidomic Analyses.

Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.) stands as one of the pivotal afforestation tree species and timber resources in southern China. Nevertheless, the occurrence of seed abortion and a notably high proportion of astringent seeds significantly curtail the yield and quality of elite seeds, resulting in substantial economic losses. The development of astringent seeds is accompanied by significant physiological and biochemical alterations. Here, the first combined lipidomic and metabolomic analysis was performed to gain a comprehensive understanding of astringent seed traits. A total of 744 metabolites and 616 lipids were detected, of which 489 differential metabolites and 101 differential lipids were identified. In astringent seeds, most flavonoids and tannins, as well as proline and γ-aminobutyric acid, were more accumulated, along with a notable decrease in lipid unsaturation, indicating oxidative stress in the cells of astringent seeds. Conversely, numerous elemental metabolites were less accumulated, including amino acids and their derivatives, saccharides and alcohols, organic acids and nucleotides and their derivatives. Meanwhile, most lipid subclasses, mainly associated with energy storage (triglyceride and diglyceride) and cell membrane composition (phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine), also exhibited significant reductions. These results reflected a disruption in the cellular system or the occurrence of cell death, causing a reduction in viable cells within astringent seeds. Furthermore, only one lipid subclass, sphingosine phosphate (SoP), was more accumulated in astringent seeds. Additionally, lower accumulation of indole-3-acetic acid and more accumulation of salicylic acid (SA) were also identified in astringent seeds. Both SA and SoP were closely associated with the promotion of programmed cell death in astringent seeds. Collectively, our study revealed significant abnormal changes in phytohormones, lipids and various metabolites in astringent seeds, allowing us to propose a model for the development of astringent seeds in Chinese fir based on existing research and our findings. This work enriches our comprehension of astringent seeds and presents valuable bioindicators for the identification of astringent seeds.

Peers influence the tobacco and alcohol use of Chinese adolescents.

This two-wave longitudinal study examined peer selection and influence pertaining to tobacco and alcohol use by adolescents and their friends in a sample of 854 Chinese adolescents (384 girls: mean age = 13.33 years). Participants nominated friends and self-reported their tobacco and alcohol use at seventh and again at eighth grade. Longitudinal social network analyses revealed evidence of friend influence but not selection over smoking and drinking. Boys increased their levels of smoking at rates greater than that of girls, but no sex moderation of either selection or influence was found. In interpreting these results, it is important to understand the gender norms for Chinese boys and girls and the cultural context of tobacco and alcohol use.

Peer Relationships and Indonesian Muslim Adolescents' Religiosity and Religious Coping: Selection and Influence.

The religious similarity of adolescents and their friends can arise from selection or influence. Prior studies were limited because of confounds that arose from the ethnic and religious heterogeneity of the samples and the use of cross-sectional designs. SIENA was used in this two-year longitudinal study of 825 Indonesian Muslim high school students (445 girls; mean age = 16.5 years) to assess peer selection and influence as these pertained to religiosity and religious coping. The analyses yielded significant influence but not selection effects for both religiosity and religious coping. This study is an important methodological advance over prior research and although limited by correlational data, nevertheless, provides evidence that adolescents influence their peers' religiousness.

Tobacco and Alcohol Use by Muslim Indonesian Adolescents: Longitudinal Associations With Religiosity and Problem Behavior.

OBJECTIVE: This three-wave (10th to 12th grade) longitudinal study explored the interrelations of religiosity, tobacco and alcohol use, and problem behavior in Indonesian Muslim adolescents. METHOD: The sample included 721 Muslim Indonesian adolescents (48% girls) who participated in at least one assessment when they were in 10th, 11th, or 12th grade. Of these, 499 were recruited in 10th grade, with others added at 11th and 12th grades. RESULTS: Prior month use for 10th-grade boys and girls, respectively, was 49.2% and 5.2% for tobacco and 19.0% and 3.6% for alcohol. Tobacco use and alcohol use were negatively associated with religiosity for boys, but these associations were more inconsistent for girls. Consistent with U.S. findings, there was a bidirectional longitudinal association between tobacco and alcohol use. The longitudinal models and associations with problem behavior were similar for both sexes. CONCLUSIONS: These results provide evidence of negative longitudinal associations between religiosity and tobacco use. The more frequent use of tobacco than alcohol may be attributable to religion, cost differentials, and social norms. Despite large gender differences in the frequencies of use, the similar predictors of tobacco and alcohol use for boys and girls suggest that models accounting for substance use are comparable for both sexes. These results have implications for the design of prevention programs in Indonesia and other culturally and religiously similar countries.

Chinese adolescents' positive relationships with parents: The effects of the sex of their younger sibling.

We investigated how sibling status and sex of younger siblings influence Chinese adolescents' relationships with their fathers and mothers as a function of resource dilution and preference for sons. The sample included 1,093 adolescents from the 8th grade (Mage  = 13.96 years, SD = 0.75 years; 47% girls) and the 11th grade (Mage  = 17.25 years, SD = 0.74 years; 57% girls) in a longitudinal study. Consistent with expectations pertaining to the hypothesis of resource dilution and son preference in combination, girls with a younger brother reported less positive relationships with mothers than either singleton girls or girls with a younger sister. No significant difference was found between singleton boys and boys with a younger sibling. The current findings show implications that the resource-dilution theory and son preference culture together may place girls with a younger brother in an unfavorable condition of resource allocation.

Religiosity and Effortful Control as Predictors of Indonesian Adolescents' Tobacco and Alcohol Use: Moderation and Mediation.

Moderation and mediation models of religiosity and effortful control as predictors of tobacco and alcohol use were tested in this 2-year longitudinal study of 563 16-year-old Muslim Indonesian adolescents. Adolescents reported their effortful control, religiosity, and tobacco and alcohol use and peers provided reports of adolescents' effortful control. Although both moderation and mediation effects emerged when predicting Year 2 substance use, predictions of change from Year 1 to Year 2 substance use yielded effects of moderation for peer- but not self-reported effortful control for boys; no mediation effects emerged. These findings provide evidence of interconnections between effortful control and religiosity as predictors of substance use and suggest the need for further longitudinal studies that compare moderation and mediation models.