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1.
Front Physiol ; 9: 282, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29662456

RESUMO

Emerging evidence indicates the beneficial effects of physical exercise on human health, which depends on the intensity, training time, exercise type, environmental factors, and the personal health status. Conventional biomarkers provide limited insight into the exercise-induced adaptive processes. Circulating microRNAs (miRNAs, miRs) are dynamically regulated in response to acute exhaustive exercise and sustained rowing, running and cycling exercises. However, circulating miRNAs in response to long-term basketball exercise remains unknown. Here, we enrolled 10 basketball athletes who will attend a basketball season for 3 months. Specifically, circulating miRNAs which were involved in angiogenesis, inflammation and enriched in muscle and/or cardiac tissues were analyzed at baseline, immediately following acute exhaustive exercise and after 3-month basketball matches in competitive male basketball athletes. Circulating miR-208b was decreased and miR-221 was increased after 3-month basketball exercise, while circulating miR-221, miR-21, miR-146a, and miR-210 were reduced at post-acute exercise. The change of miR-146a (baseline vs. post-acute exercise) showed linear correlations with baseline levels of cardiac marker CKMB and the changes of inflammation marker Hs-CRP (baseline vs. post-acute exercise). Besides, linear correlation was observed between miR-208b changes (baseline vs. after long-term exercise) and AT VO2 (baseline). The changes of miR-221 (baseline vs. after long-term exercise) were significantly correlated with AT VO2, peak work load and CK (after 3-month basketball matches). Although further studies are needed, present findings set the stage for defining circulating miRNAs as biomarkers and suggesting their physiological roles in long-term exercise training induced cardiovascular adaptation.

2.
Anal Chim Acta ; 992: 85-93, 2017 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-29054153

RESUMO

A new strategy is proposed based on inhibition of ion transport by lipid bilayer derived from spontaneous assembly of lipopolysaccharides (LPS), thereby a colorimetric method is established for analysis of LPS. At acidic pH values, LPS can specially bind with aminophenylboronic acid modified assembled magnetic nanospheres (APBA/AMNSs), resulting in formation of lipid bilayer around APBA/AMNSs. Under acidic condition, the lipid bilayer can inhibit the release of iron ions from AMNSs into the solution so as to decrease the oxidized extent of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt mediated by hydrogen peroxide. Using the established method, LPS can be detected over the wide linear detection range with the low detection limit. With good selectivity, reproductivity, and simplicity, the method is accurate in LPS tests of real drinking samples.


Assuntos
Bebidas/análise , Colorimetria , Lipopolissacarídeos/análise , Peróxido de Hidrogênio , Transporte de Íons , Ácidos Sulfônicos/química
3.
J Nanosci Nanotechnol ; 15(2): 1110-6, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26353619

RESUMO

This report investigated the effect of carbon nanomaterials, single-wall carbon nanotube (SWCNT) and graphene oxide, on fibrillation of ß-amyloid 40 (Aß40) based on surface plasmon resonance (SPR) and molecular dynamics (MD). MD simulations are carried out in order to reveal the molecular mechanisms of the interaction between nanomaterials and Aß40. The strong interaction between Aß40 and nanomaterials is related to Van der Waals forces and the Coulomb force, inducing delicate manipulation of the main bonding energy for fibrillation of Aß40. The interaction energy between the Aß peptide and graphene is higher than that of SWCNT. Experimental results show both carbon nanomaterials enhance the appearance of a critical nucleus for nucleation of peptide fibrils. Graphene is more beneficial to assist the nucleation process than SWCNT. Combination of SPR and molecular dynamics could be a high-throughput method to screen protein fibrillation.


Assuntos
Peptídeos beta-Amiloides/química , Amiloide/química , Amiloide/ultraestrutura , Simulação de Dinâmica Molecular , Nanopartículas/química , Fragmentos de Peptídeos/química , Ressonância de Plasmônio de Superfície/métodos , Peptídeos beta-Amiloides/ultraestrutura , Sítios de Ligação , Simulação por Computador , Teste de Materiais , Modelos Químicos , Complexos Multiproteicos/síntese química , Complexos Multiproteicos/ultraestrutura , Nanopartículas/ultraestrutura , Fragmentos de Peptídeos/ultraestrutura , Ligação Proteica
5.
Biosens Bioelectron ; 61: 83-7, 2014 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-24858995

RESUMO

Vascular endothelial growth factor (VEGF) is a major regulator of angiogenesis. It has been identified as an ideal biomarker for staging of many kinds of cancers, so more specific and intense signal is desirable for VEGF biosensors so that the sensors may have more valuable clinical application. Herein, we report a highly sensitive and selective surface plasmon resonance (SPR) sensor for VEGF detection by using two DNA aptamers which target different VEGF domains used as the capture and detection probe, respectively. Moreover, by making use of carboxyl-coated polystyrene microspheres, 3'-NH2 immobilized aptamer and 3'-NH2 modified primer DNA are loaded through amidation onto the sensing layer for further rolling circle amplification (RCA) process to amplify the SPR signal. With the well-designed sensing platform, VEGF can be determined in a linear range from 100 pg mL(-1) to 1 µg mL(-1) with a detection limit of 100 pg mL(-1). Due to its high specificity and desirable sensitivity, this RCA assisted SPR method may be a useful tool for the assay of VEGF in the future. What is more, by replacing the sensing element, i.e., the aptamer of VEGF used in this work, more biosensors for sensitive detection of other biomarkers proteins can be fabricated based on the strategy proposed in this study.


Assuntos
Aptâmeros de Nucleotídeos/química , Ressonância de Plasmônio de Superfície/instrumentação , Fator A de Crescimento do Endotélio Vascular/análise , Desenho de Equipamento , Limite de Detecção , Hibridização de Ácido Nucleico/métodos , Ressonância de Plasmônio de Superfície/métodos
6.
ACS Appl Mater Interfaces ; 6(10): 7070-5, 2014 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-24786305

RESUMO

In this paper, we have proposed a new electrochemical aptasensor based on a novel ligase-assisted Exo III-catalyzed degradation reaction (LAECDR), which consists of DNA ligase-catalyzed ligation of thrombin-binding aptamer (TBA) with an extension strand (E-strand) and Exo III-catalyzed selective degradation of probe DNA, by using an improved target-induced strand displacement strategy. As a result of LAECDR, methylene blue (MB)-labeled mononucleotides can be released from the 3'-terminal of probe DNA and captured by cucurbit[7]uril-functionalized electrode to induce noticeable electrochemical response. Nevertheless, in the presence of the target protein, thrombin, the TBA that is partially complementary to probe DNA is preferentially binding with the target protein, thereby inhibiting LAECDR from taking place. The remaining intact probe DNA will prevent the terminal-attached MB from approaching to the electrode surface due to strong electrostatic repulsion, so the electrochemical response will be changed by thrombin. By tracing the electrochemical response of adsorbed MB, our aptasensor can exhibit high sensitivity for thrombin detection with a wide linear range from 100 fM to 1 nM and an extremely low detection limit of 33 fM, which can also easily distinguish thrombin in the complex serum samples with high specificity. Therefore, our aptasensor might have great potential in clinical applications in the future.


Assuntos
Aptâmeros de Nucleotídeos/química , DNA Ligases/metabolismo , Exodesoxirribonucleases/metabolismo , Hidrocarbonetos Aromáticos com Pontes/química , Sondas de DNA/metabolismo , Técnicas Eletroquímicas , Eletrodos , Exodesoxirribonucleases/química , Ouro/química , Imidazóis/química , Nanopartículas Metálicas/química , Azul de Metileno/química , Ligação Proteica , Trombina/análise , Trombina/metabolismo
7.
Biosens Bioelectron ; 47: 32-7, 2013 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-23542067

RESUMO

Graphene, a single atom thick and two dimensional carbon nano-material, has been proven to possess many unique properties, one of which is the recent discovery that it can interact with single-stranded DNA through noncovalent π-π stacking. In this work, we demonstrate that a new strategy to fabricate many kinds of biosensors can be developed by combining this property with cascade chemical reactions. Taking the fabrication of glucose sensor as an example, while the detection target, glucose, may regulate the graphene-DNA interaction through three cascade chemical reactions, electrochemical techniques are employed to detect the target-regulated graphene-DNA interaction. Experimental results show that in a range from 5µM to 20mM, the glucose concentration is in a natural logarithm with the logarithm of the amperometric response, suggesting a best detection limit and detection range. The proposed biosensor also shows favorable selectivity, and it has the advantage of no need for labeling. What is more, by controlling the cascade chemical reactions, detection of a variety of other targets may be achieved, thus the strategy proposed in this work may have a wide application potential in the future.


Assuntos
Técnicas Biossensoriais/instrumentação , DNA/química , Glucose/isolamento & purificação , Grafite/química , Carbono/química , Técnicas Eletroquímicas , Nanoestruturas/química , Óxidos/química
8.
Biosens Bioelectron ; 46: 108-12, 2013 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-23517826

RESUMO

Protein-DNA interaction plays important roles in many cellular processes, and there is an urgent demand for valid methods to monitor the interaction. In view of this, we propose a simple label-free colorimetric platform for the detection of protein-DNA interaction. Protein-DNA couples together with peroxidase-mimicking DNAzyme and exonuclease are elaborately incorporated into an integrated biosensing system. Besides the simplicity and efficiency, the strategy also has a great advantage for its universality in the detection of different protein-DNA couples. In our experiments, effective validation of our approach can be supported by two different protein-DNA couples (estrogen receptor α and nuclear factor kappa B). Experimental results show that the DNAzyme is competent to give rise to evident readout signals to monitor protein-DNA couples. Furthermore, with the substitution of DNA binding sequence in the probe, this method could be extended to a general platform for the detection of protein-DNA interaction.


Assuntos
Técnicas Biossensoriais/métodos , Colorimetria/métodos , DNA/metabolismo , Receptor alfa de Estrogênio/metabolismo , NF-kappa B/metabolismo , Animais , Benzotiazóis/metabolismo , Técnicas Biossensoriais/economia , Colorimetria/economia , DNA Catalítico/metabolismo , Exodesoxirribonucleases/metabolismo , Quadruplex G , Humanos , Peroxidase/metabolismo , Ácidos Sulfônicos/metabolismo
9.
Anal Chim Acta ; 764: 59-63, 2013 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-23374215

RESUMO

Breast cancer is one of the most critical threats to the health of women, and the development of new methods for early diagnosis is urgently required, so this paper reports a method to detect Michigan cancer foundation-7 (MCF-7) human breast cancer cells with considerable sensitivity and selectivity by using electrochemical technique. In this method, a mucin 1 (MUC1)-binding aptamer is adopted to recognize MCF-7 human breast cancer cells, while enzyme labeling is employed to produce amplified catalytic signals. The molecular recognition and the signal amplification are elaborately integrated by fabricating an aptamer-cell-aptamer sandwich architecture on an electrode surface, thus a biosensor for the detection of MCF-7 is fabricated based on the architecture. The detection range can be from 100 to 1×10(7) cells, and the detection limit can be as low as 100 cells. The method is also cost-effective and conveniently operated, implying potential help for the development of early diagnosis of breast cancer.


Assuntos
Aptâmeros de Nucleotídeos/metabolismo , Técnicas Biossensoriais , Técnicas Eletroquímicas , Mucina-1/metabolismo , Aptâmeros de Nucleotídeos/química , Neoplasias da Mama/diagnóstico , Espectroscopia Dielétrica , Detecção Precoce de Câncer , Eletrodos , Feminino , Peroxidase do Rábano Silvestre/metabolismo , Humanos , Células MCF-7 , Mucina-1/química , Propriedades de Superfície
10.
Anal Chem ; 83(11): 4085-9, 2011 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-21545113

RESUMO

A novel strategy to fabricate an aptasensor for potassium with high sensitivity and selectivity by using nicking endonuclease is proposed in this work. A nicking endonuclease (Nt.CviPII), which may recognize specific nucleotide sequences in double-stranded DNA formed by a potassium-binding aptamer and a linker DNA but cleave only the linker strand, may transfer and amplify the quantitative information of the potassium detection to that of the linker DNA through elaborate strand-scission cycles. Since the technique for gene assay is much more mature, the linker DNA can thereby be detected by a number of available methods. Here, taking advantage of a simple and fast gold nanoparticles-based sensing technique, we are able to assay the linker and consequently potassium ion simply by UV-vis spectroanalysis and even with the naked eye. Results show that a 2 µL sample containing 0.1 mM of potassium is enough to induce distinct color appearance of the nanoparticles, and the potassium ion can be easily distinguished from many other ions. The strategy proposed in this work shows some unique advantages over some traditional methods and may be further developed for the detection of some other chemicals in the future.


Assuntos
Aptâmeros de Nucleotídeos/química , Colorimetria/métodos , DNA/química , Endonucleases/metabolismo , Potássio/análise , Ouro/química , Nanopartículas Metálicas/química
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