[Effect of dithiothreitol on the deformation and La(3+)-induced fusion of human erythrocytes].

The effect of dithiothreitol on the deformation and La(3+)-induced fusion of human erythrocytes was studied. It was shown that dithiothreitol did not affect the deformation of erythrocytes. At the same time, dithiothreitol practically completely inhibited the fusion of erythrocytes.

[La(3+)-induced fusion of plant protoplasts].

The effect of La(3+) on the fusion of plant protoplasts has been studied. It was shown that La(3+) induced the aggregation of plant protoplasts. The incubation of a suspension of aggregated protoplasts at 42 degrees C for 30 min resulted in their fusion.

[A study of the aggregation of human red blood cells induced by picric acid].

The effect of picric acid on the aggregation of human erythrocytes was studied. It was shown that the addition of picric acid to a suspension of washed erythrocytes leads to a decrease in pH of medium to 1.5-2 and the formation of echinocytes. Stirring the suspension of echinocytes at low pH values results in a strong aggregation of cells. Increasing the pH value to 7.4 leads to a desaggregation of echinocytes. It was found that picric acid does not induce the aggregation of cells fixed by glutaraldehyde. A substantial decrease in the aggegation of spheric erythrocytes obtained after heating the cells at 50 degrees C was observed.

[Effect of diamide on deformability and La(3+)-induced aggregation and fusion of human erythrocytes]. / Vliianie diamida na deformiruemost' i La(3+)-indutsiruemye agregatsiiu i slianie éritrotsitov cheloveka.

The effect of diamide on the deformability and La(3+)-induced aggregation and fusion of human erythrocytes was studied. It was shown that diamide decreased the deformability of erythrocytes and practically completely inhibited their fusion. It was found that diamide did not change the aggregation of erythrocytes.

[Effect of La(3+) on electrophoretic mobility and aggregation of intact human erythrocytes and those treated with low concentrations of glutaric aldehyde]. / Vliianie La(3+) na élektroforeticheskuiu podvizhnost' i agregatsiiu intaktnykh i obrabotannykh nizkimi kontsentratsiiami glutarovogo al'degida éritrotsitov cheloveka.

It was shown that the treatment of erythrocytes with low concentrations of glutaraldehyde (0.01-0.1%) for 30-120 min to a variable extent the aggregation induced by 40-330 microM La3+. The effect of glutaraldehyde on the aggregation increased with concentration and time of fixation. La3+ ions decreased to a similar extent the electrophoretic mobility of intact erythrocytes and erythrocytes treated with 0.1% glutaraldehyde. No relationship was found between the change in the negative charge on the erythrocytes and the degree of their aggregation. Neuraminidase and trypsin were shown to decrease the surface charge on the erythrocytes and the aggregation of fixed erythrocytes.

[Determination with a microelectrode of membrane potential of giant human erythrocytes obtained by La(3+)-induced fusion]. / Mikroélektrodnoe izmerenie membrannogo potsentsiala gigantskikh éritrotsitov cheloveka, poluchennykh putem La(3+)-indutsirovannogo sliianiia.

The effect of La3+ on the fusion of erythrocytes of blood stored for a week at +4 degrees C was studied. It was shown that the fusion of erythrocytes begins after one day of storage of blood. The most intensive fusion of erythrocytes was observed on day 4 of blood storage. As a result, giant cells with a size of 100 microns and more arise. The electrical potential of giant cells was measured using a microelectrode and was -6.6 +/- 1.5 mV.

[Effect of intracellular ATP on La(3+)-induced aggregation and fusion of human erythrocytes]. / Vliianie vnutrikletochnogo soderzhaniia ATF na indutsiruemye La(3+) agregatsiiu i sliianie éritrotsitov cheloveka.

The influence of intracellular ATP level on the aggregation and fusion of human erythrocytes, induced by La3+ in the concentration range 20-330 microM was studied. The aggregation of intact red blood cells differs from that of cells with increased and decreased contents of ATP. Incubation of erythrocyte aggregates at 37 degrees C did not lead to cell fusion. At the same time, incubation of erythrocyte aggregates with decreased and increased ATP contents in the presence of La3+ induced a pronounced cell fusion.

[Effect of La2+ on the form, aggregation, and fusion of human erythrocytes]. / Vliianie La2+ na formu, agregatsiiu i sliianie éritrotsitov cheloveka.

By optical microscopy, it has been shown that the addition of La3+ ions induced transformation in the shape of erythrocytes, their aggregation and fusion. After addition of La3+ erythrocytes transform into stomatocytes. It was found that the red cell shape recovered to discoid after addition of EDTA. Neither transformation of shape nor aggregation or fusion of erythrocytes could be detected after their treatment with glutaraldehyde. A possible mechanism and significance of the shape transformation in aggregation of erythrocytes is discussed.

[Effect of neuraminidase and proteolytic enzymes on electrophoretic mobility of erythrocytes and their aggregation induced by La(3+)]. / Vliianie neiraminidazy i proteoliticheskikh fermentov na électroforeticheskuiu podvizhnost' éritrotsitov i ikh agregatsiiu, indutsiruemuiu La(3+).

The effect of neuraminidase, trypsin and pronase on the electrophoretic mobility of human erythrocytes and their aggregation induced by La3+ was studied in vitro. The aggregation of intact red cells differs from that of cells treated with neuraminidase and proteolytic enzymes. The above differences are observed in the range of La3+ suspension concentration from 20 to 330 microM. A possible mechanism of aggregation is discussed.