Response of in ovo administration of zinc on egg hatchability and immune response of commercial broiler chicken.

An experiment was conducted to determine the effect of in ovo administration of different forms of zinc with respect to hatchability and performance of commercial broiler chicken. In trial 1, the fertile eggs on day 18 were divided into six treatment groups: Group I as control without any supplementation of zinc, group II to IV were supplemented with 0.5 mg zinc per egg as zinc sulphate, zinc methionine or nano zinc, respectively, and Group V with nano zinc at 0.25 mg zinc per egg. Sixth group received 0.5 ml citric acid per egg as sham control. The results of the first trial indicated that in ovo administration of nano zinc at both levels and zinc methionine resulted in complete failure of hatchability. A second trial to validate the result of trial 1 consisted of Group I control (no administration). Group II and Group III were supplemented with zinc sulphate and zinc methionine, respectively, at 0.5 mg zinc per egg. Group IV and Group V were supplemented with nano zinc at 0.04 and 0.08 mg per egg. In the second trial, again there was a similar pattern for zinc sulphate and zinc methionine. Administration of Zn by nano form had around 80% hatchability on fertile eggs in comparison with the unadministered control eggs (92%). There was no difference (p > .05) in body weight gain, feed intake and FCR. No difference (p > .05) was observed between treatments for cell-mediated immune response and humoral immune response. Nano Zn-administered group showed a non-significant downregulation of MUC2 gene. It could be concluded that in ovo administration of higher levels of zinc has to be with caution for the developing embryo of commercial broiler chicken.

Effect of super dosing of phytase on growth performance, ileal digestibility and bone characteristics in broilers fed corn-soya-based diets.

A feeding trial was designed to assess the effect of super dosing of phytase in corn-soya-based diets of broiler chicken. One hundred and sixty-eight day-old broilers were selected and randomly allocated to four dietary treatment groups, with 6 replicates having 7 chicks per treatment group. Two-phased diets were used. The starter and finisher diet was fed from 0 to 3 weeks and 4 to 5 weeks of age respectively. The dietary treatments were consisted of normal phosphorus (NP) group without any phytase enzyme (4.5 g/kg available/non-phytin phosphorus (P) during starter and 4.0 g/kg during finisher phase), three low-phosphorus (LP) groups (3.2 g/kg available/non-phytin P during starter and 2.8 g/kg during finisher phase) supplemented with phytase at 500, 2500, 5000 FTU/kg diet, respectively, to full fill their phosphorus requirements. The results showed that super doses of phytase (at 2500 FTU and 5000 FTU/kg) on low-phosphorus diet improved feed intake, body weight gain, ileal digestibility (serine, aspartic acid, calcium, phosphorus), blood P levels and bone minerals such as calcium (Ca), P, magnesium (Mg) and zinc (Zn) content. It could be concluded that super doses of phytase in low-phosphorus diet were beneficial than the normal standard dose (at 500 FTU/kg) of phytase in diet of broiler chicken.

Risk factors for 3rd and 4th degree perineal tear.

We reviewed 3,038 deliveries at our hospital, over a period of 2 years (2005 and 2006) to identify risk factors for 3rd and 4th degree perineal tear. We used the hospital database and labour ward registry book and reviewed patients' record notes. After excluding elective and emergency caesarean sections, 2,278 women had delivered vaginally, from which 36 patients had 3rd/4th degree perineal tears as defined by the RCOG Green top guidelines No 29 (2007). A total of 2,242 women who delivered vaginally without 3rd/4th degree perineal tears were used as controls in this study. The rate of 3rd/4th degree perineal tear was 1.18% for all deliveries and 1.58% for vaginal deliveries. Occiptoposterior position during delivery (OR: 69.8), primigravida (OR: 5.8), and high birth weight (OR: 1.19) are risk factors for anal sphincter tear. However, induction of labour (OR: 0.71), use of medio-lateral episiotomy (OR: 0.35), epidural analgesia (OR: 0.88) and instrumental delivery of occipitoanterior position (OR: 0.77) reduced the risk of severe perineal tear. Primipara and occipitoposterior position (OP) during delivery are the only statistically significant risks for the occurrence of severe perineal damage. High birth weight is a risk factor but it is not statistically significant. Instrumental delivery of OP position is a highly statistically significant risk factor. On the other hand, induction of labour (IOL), epidural analgesia and instrumental delivery for occipitoanterior position are protective factors against anal sphincter injury, although they did not reach statistical significance.