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1.
Mol Syst Biol ; 5: 300, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19690569

RESUMO

A transcriptional activator can suppress gene expression by interfering with transcription initiated by another activator. Transcriptional interference has been increasingly recognized as a regulatory mechanism of gene expression. The signals received by the two antagonistically acting activators are combined by the polymerase trafficking along the DNA. We have designed a dual-control genetic system in yeast to explore this antagonism systematically. Antagonism by an upstream activator bears the hallmarks of competitive inhibition, whereas a downstream activator inhibits gene expression non-competitively. When gene expression is induced weakly, the antagonistic activator can have a positive effect and can even trigger paradoxical activation. Equilibrium and non-equilibrium models of transcription shed light on the mechanism by which interference converts signals, and reveals that self-antagonism of activators imitates the behavior of feed-forward loops. Indeed, a synthetic circuit generates a bell-shaped response, so that the induction of expression is limited to a narrow range of the input signal. The identification of conserved regulatory principles of interference will help to predict the transcriptional response of genes in their genomic context.


Assuntos
Ativação Transcricional , Ligação Competitiva , DNA/genética , Citometria de Fluxo , Expressão Gênica , Regulação Fúngica da Expressão Gênica , Genoma Fúngico , Cinética , Modelos Genéticos , Regiões Promotoras Genéticas , Estrutura Terciária de Proteína , Saccharomyces cerevisiae/genética , Transdução de Sinais , Biologia de Sistemas , Transcrição Gênica
2.
Neural Dev ; 2: 20, 2007 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-17958902

RESUMO

BACKGROUND: Odors are detected by sensory neurons that carry information to the olfactory lobe where they connect to projection neurons and local interneurons in glomeruli: anatomically well-characterized structures that collect, integrate and relay information to higher centers. Recent studies have revealed that the sensitivity of such networks can be modulated by wide-field feedback neurons. The connectivity and function of such feedback neurons are themselves subject to alteration by external cues, such as hormones, stress, or experience. Very little is known about how this class of central neurons changes its anatomical properties to perform functions in altered developmental contexts. A mechanistic understanding of how central neurons change their anatomy to meet new functional requirements will benefit greatly from the establishment of a model preparation where cellular and molecular changes can be examined in an identified central neuron. RESULTS: In this study, we examine a wide-field serotonergic neuron in the Drosophila olfactory pathway and map the dramatic changes that it undergoes from larva to adult. We show that expression of a dominant-negative form of the ecdysterone receptor prevents remodeling. We further use different transgenic constructs to silence neuronal activity and report defects in the morphology of the adult-specific dendritic trees. The branching of the presynaptic axonal arbors is regulated by mechanisms that affect axon growth and retrograde transport. The neuron develops its normal morphology in the absence of sensory input to the antennal lobe, or of the mushroom bodies. However, ablation of its presumptive postsynaptic partners, the projection neurons and/or local interneurons, affects the growth and branching of terminal arbors. CONCLUSION: Our studies establish a cellular system for studying remodeling of a central neuromodulatory feedback neuron and also identify key elements in this process. Understanding the morphogenesis of such neurons, which have been shown in other systems to modulate the sensitivity and directionality of response to odors, links anatomy to the development of olfactory behavior.


Assuntos
Sistema Nervoso Central/crescimento & desenvolvimento , Drosophila melanogaster/crescimento & desenvolvimento , Metamorfose Biológica/fisiologia , Condutos Olfatórios/crescimento & desenvolvimento , Células Receptoras Sensoriais/metabolismo , Serotonina/metabolismo , Animais , Diferenciação Celular/genética , Forma Celular/genética , Sistema Nervoso Central/citologia , Sistema Nervoso Central/metabolismo , Dendritos/metabolismo , Dendritos/ultraestrutura , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/citologia , Drosophila melanogaster/metabolismo , Ecdisterona/metabolismo , Retroalimentação/fisiologia , Cones de Crescimento/metabolismo , Cones de Crescimento/ultraestrutura , Citometria por Imagem , Larva/citologia , Larva/crescimento & desenvolvimento , Larva/metabolismo , Proteínas Associadas aos Microtúbulos/genética , Proteínas Associadas aos Microtúbulos/metabolismo , Corpos Pedunculados/citologia , Corpos Pedunculados/crescimento & desenvolvimento , Corpos Pedunculados/metabolismo , Rede Nervosa/citologia , Rede Nervosa/crescimento & desenvolvimento , Rede Nervosa/metabolismo , Plasticidade Neuronal/genética , Condutos Olfatórios/citologia , Condutos Olfatórios/metabolismo , Receptores de Esteroides/genética , Receptores de Esteroides/metabolismo , Células Receptoras Sensoriais/citologia , Olfato/genética
3.
BMC Dev Biol ; 5: 25, 2005 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-16281986

RESUMO

BACKGROUND: The development of nervous systems involves reciprocal interactions between neurons and glia. In the Drosophila olfactory system, peripheral glial cells arise from sensory lineages specified by the basic helix-loop-helix transcription factor, Atonal. These glia wrap around the developing olfactory axons early during development and pattern the three distinct fascicles as they exit the antenna. In the moth Manduca sexta, an additional set of central glia migrate to the base of the antennal nerve where axons sort to their glomerular targets. In this work, we have investigated whether similar types of cells exist in the Drosophila antenna. RESULTS: We have used different P(Gal4) lines to drive Green Fluorescent Protein (GFP) in distinct populations of cells within the Drosophila antenna. Mz317::GFP, a marker for cell body and perineural glia, labels the majority of peripheral glia. An additional approximately 30 glial cells detected by GH146::GFP do not derive from any of the sensory lineages and appear to migrate into the antenna from the brain. Their appearance in the third antennal segment is regulated by normal function of the Epidermal Growth Factor receptor and small GTPases. We denote these distinct populations of cells as Mz317-glia and GH146-glia respectively. In the adult, processes of GH146-glial cells ensheath the olfactory receptor neurons directly, while those of the Mz317-glia form a peripheral layer. Ablation of GH146-glia does not result in any significant effects on the patterning of the olfactory receptor axons. CONCLUSION: We have demonstrated the presence of at least two distinct populations of glial cells within the Drosophila antenna. GH146-glial cells originate in the brain and migrate to the antenna along the newly formed olfactory axons. The number of cells populating the third segment of the antenna is regulated by signaling through the Epidermal Growth Factor receptor. These glia share several features of the sorting zone cells described in Manduca.


Assuntos
Neuroglia/citologia , Neurônios Receptores Olfatórios/citologia , Animais , Encéfalo/citologia , Movimento Celular , Drosophila , Receptores ErbB/fisiologia , Condutos Olfatórios/citologia , Transdução de Sinais
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