RESUMO
OBJECTIVE: To explore the correlation of matrix metalloproteinase-1 (MMP(1)) and tissue inhibitor of metalloproteinase-1 (TIMP(1)) with pituitary adenoma fibrosis. METHODS: Thirty-eight pituitary adenoma specimens were divided into fibrous group (6 patients) and non-fibrous group (32 patients). MMP(1) and TIMP(1) expression was detected by RT-PCR and immunohistochemistry. The collagen content was determined by Sirius scarlet staining. RESULTS: In fibrous and non-fibrous group: (1) the collagen content was 20.95 +/- 8.42% and 7.98 +/- 5.18% respectively, and there was a statistical significance (P < 0.01). (2) The expression of MMP(1)mRNA was 0.47 +/- 0.40 and 0.59 +/- 0.54 respectively and its protein expression was 0.12 +/- 0.09 and 0.13 +/- 0.09 respectively, and there was no statistical significance (P > 0.05). Their expression was not related to collagen content (P > 0.05). (3) The expression of TIMP(1)mRNA was 1.61 +/- 1.09 and 0.79 +/- 0.59 respectively and its protein expression was 0.58 +/- 0.11 and 0.32 +/- 0.18 respectively, and there was a statistical significance (P < 0.01). Their expression was related to collagen content (P < 0.01). CONCLUSION: The pathological features of pituitary adenoma fibrosis are excessive collagen deposition. High expression of TIMP(1) may be an important cause.
Assuntos
Adenoma/metabolismo , Metaloproteinase 1 da Matriz/metabolismo , Neoplasias Hipofisárias/metabolismo , Inibidor Tecidual de Metaloproteinase-1/metabolismo , Adenoma/patologia , Adulto , Idoso , Colágeno/metabolismo , Feminino , Fibrose , Expressão Gênica , Humanos , Masculino , Metaloproteinase 1 da Matriz/genética , Pessoa de Meia-Idade , Neoplasias Hipofisárias/patologia , RNA Mensageiro/metabolismo , Estudos Retrospectivos , Inibidor Tecidual de Metaloproteinase-1/genética , Adulto JovemRESUMO
OBJECTIVE: To investigate the expressions of stromal cell-derived factor-1 (SDF-1) and CX chemokine receptor-4 (CXCR-4) in patients with hepatocellular carcinoma (HC) and liver cirrhosis. METHODS: Peripheral blood and/or ascites fluid were collected from 39 hepatocellular carcinoma patients, 16 patients with liver cirrhosis, 12 with hepatitis and 12 healthy donors. The SDF-1 expression was assayed by ELISA and CXCR-4 was measured by immunohistochemical methods. RESULTS: The level of SDF-1 expression in the carcinoma patients was higher than that of the liver cirrhosis, hepatitis patients and healthy donors, but there was no significant difference between those of the healthy donors and hepatitis patients or liver cirrhosis patients. The levels of CXCR-4 expression were closely related to the tumor differentiation. CONCLUSION: The expression of SDF-1 in the peripheral blood and the CXCR4 expression in the HCC tissues of the HC patients may be regarded as markers of HC and they may have a positive relationship with the differentiation and metastasis of HC.
