[Effects on the survival of rat's abdominal axial skin flap after the combination of VEGF gene therapy and skin flap delay].

OBJECTIVE: To investigate the effect of VEGF (vascular endothelial growth factor) gene therapy and skin flap delay on the survival of rat's abdominal axial skin flap. METHODS: In 48 Wistar rats, the model of a abdominal axial skin flap supplied by right superficial epigastric vessel was created. The rats were divided into six groups. The group was treated with subcutaneous injection of pcDNA4-VEGF165, skin flap delay or VEGF injection combined with skin flap delay. 7 days later, the survival rate of the skin flap was measured; specimens were harvested from the skin flap for histological investigation of the microvessels and for immunohistochemical staining to observe the expression of VEGF. RESULTS: Every treated group was significantly higher than blank group in the average survival rate of the skin flap and group V (gene injection when delayed) has the highest one. The average number of the microvessels in group II, III, V, VI was significantly higher than group IV and blank group. Group IV > group V, VI > group II, III > blank group in lumen diameter of the microvessels. Immunohistochemical staining documented more deposition of VEGF DNA in group II, III, V, VI than group IV and blank group. CONCLUSIONS: Both administration of pcDNA4-VEGF165 and skin flap delay can improve the survival of rat's abdominal axial skin flap, but the mechanisms of the effect were different. The combination of the two ways has stronger effect.

[Analysis of some related factors of effect on gluteus muscle contraction].

OBJECTIVE: To study some related factors of effect on gluteus muscle contraction and provide the therapeutic basis. METHODS: The curative effect was assessed in 154 patients who were classified by age, patient's condition, orthopedic degree in operation and rehabilitation with an average follow-up period of 25 months(ranging from 5 to 36 months). RESULTS: The excellent rate of 18-24 years old (25/30) was lower than that of 5-17 years old(120/124) (P < 0.05); the excellent rate of slight patients was higher (107/109) than that of serious patients (38/45) (P < 0.01); the excellent rate from higher orthopedic degree was higher (111/113) than that from lower orthopedic degree (34/41) (P < 0.01); and the excellent rate of rehabilitation was much higher (107/110) than that of general treatment (38/44) (P < 0.05). CONCLUSION: Age, patient's condition, orthopedic degree in operation and rehabilitation are important factors to affect the curative effect on gluteu muscle contraction.