Effect of Systematic Holistic Nursing Combined with the MDT Teaching Method in the Nursing of Neonatal Jaundice and Its Impact on the Recovery of the Newborns' Physiological Function.

The application effect of systematic holistic nursing combined with the multidisciplinary team (MDT) in the nursing of neonatal jaundice was explored. This study was a retrospective control study. 90 cases of neonatal jaundice admitted to our hospital (February 2020-February 2021) were equally split into group P treated with routine nursing and group Q treated with systematic holistic nursing combined with MDT. The application effect of the two nursing programs was compared and analyzed. Groups P and Q showed no statistical difference in general data (P > 0.05). Compared with group P, the jaundice regression time, hospitalization time, time of first defecation, and time of meconium turning yellow of group Q were notably shorter, and the body weight and total treatment efficiency of group Q were notably higher (P < 0.05). From the third day, the daily jaundice indexes between the two groups were different; that is, the indexes of group Q were notably lower compared with group P (P < 0.05). The scores of environmental nursing, special nursing, basic nursing, and service attitude in group Q were notably higher compared with group P (P < 0.05). In the nursing process of neonatal jaundice, the combination of systematic holistic nursing and MDT can effectively shorten the time of first defecation and meconium turning yellow, reduce jaundice indexes, promote the recovery of the physiological function, and improve the clinical efficacy and nursing quality.

circCUX1 promotes neuroblastoma progression and glycolysis by regulating the miR-338-3p/PHF20 axis.

Neuroblastoma (NB) is an extracranial solid malignancy in childhood. More and more studies have demonstrated that circRNAs are essential regulators of various tumors. This study conducted to explore the role and mechanism of circular RNA CUT-like homeobox 1 (circCUX1) in NB. The levels of circCUX1, miR-338-3p and plant homeodomain finger protein 20 (PHF20) were detected by qRT-PCR or Western blot. Cell proliferation and apoptosis were evaluated by colony formation assay, flow cytometry and Western blot analysis. Cell migration and invasion were examined via transwell assay. Glycolysis was expressed by measuring the extracellular acidification rate (ECAR). The interaction among circCUX1, miR-338-3p and PHF20 were validated by dual-luciferase reporter assay and RNA Immunoprecipitation assay. Besides, xenograft experiment was performed to assess tumor growth in vivo. circCUX1 and PHF20 were up-regulated, while miR-338-3p was down-regulated in NB tissues and cells. Knockdown of circCUX1 suppressed the progression and glycolysis of NB cells. circCUX1 triggered NB progression and glycolysis by regulating miR-338-3p. Additionally, down-regulation of miR-338-3p promoted NB progression and glycolysis via targeting PHF20. Moreover, circCUX1 sponged miR-338-3p to regulate PHF20 expression. Furthermore, circCUX1 silencing hindered tumor growth in vivo. circCUX1 depletion suppressed tumor progression and glycolysis in NB by regulating miR-338-3p/PHF20 axis, suggesting a potential biomarker for NB treatment.

Multisynergistic Platform for Tumor Therapy by Mild Microwave Irradiation-Activated Chemotherapy and Enhanced Ablation.

Microwave (MW) therapy, as a promising type of thermal therapy, has been attracting more and more attention from scientists. The combination of thermal and chemotherapy is of great significance in the latest studies of synergistic tumor therapy. However, the research on the MW therapy mechanism, especially the nonthermal effect applied in the combined cancer therapy, is not thorough enough. Pleasantly, we have discovered that nonthermal MW irradiation can promote the cellular uptake of nanoparticles and anticancer drugs via experiments in vitro and in vivo. Therefore, multifunctional nanoplatforms have been designed for enhanced tumor inhibition by loading ionic liquids (ILs), doxorubicin hydrochloride (DOX), and phase change materials (PCMs) into ZrO2 hollow nanoparticles. PCMs act as MW switches. The as-made IL-DOX-PCM@ZrO2 nanoplatforms were injected into H22-tumor-bearing mice via the tail vein. Mild microwave irradiation (0.9 W, 450 MHz) was then applied. The thermal effect of MW could cause the temperature of the tumor site to rise (58 °C). On the other hand, it will trigger the MW switch to open and release DOX when the temperature is high enough. At the same time as drug release, a MW nonthermal effect could improve the cellular uptake of nanomaterials and anticancer drugs. The multisynergistic effect can promote the survival rate of the IL-DOX-PCM@ZrO2+MW group to 100%. The results of the tumor therapy experiment in vivo demonstrated that as-made multifunctional IL-DOX-PCM@ZrO2 nanoplatforms could enhance the therapeutic outcome of combined thermal and chemotherapy under mild MW irradiation.

Highly stable microwave susceptible agents via encapsulation of Ti-mineral superfine powders in urea-formaldehyde resin microcapsules for tumor hyperthermia therapy.

In this study, Ti-mineral superfine powders (Ti-MSP) encapsulated in urea-formaldehyde resin microcapsules (Ti-MSP@UF-MC) were successfully prepared via a one-step microemulsion method for the first time. Because of the strong confinement effects, the Ti-MSP@UF-MC possessed perfect microwave heating effects. The temperature was 9.3 °C higher than that of the saline solution, superior to UF-MC (no significant microwave heating effect, 0 °C) and Ti-MSP (5.1 °C). The Ti-MSP@UF-MC showed low toxicity and good biocompatibility via a series of studies, including a hemolysis study and the MTT assay in vitro and in vivo. When the concentration was below 1000 µg mL(-1), the hemolysis rate was lower than 5% (hemolysis study). When the concentration was below 400 µg mL(-1), the cell activity was higher than 80% (MTT assay). Moreover, the Ti-MSP@UF-MC exhibited an ideal CT imaging effect in vivo owing to the large molecular weight of Ti-MSP. The Ti-MSP@UF-MC showed a favorable microwave therapy effect in vivo. Using mice bearing H22 tumor cells as an animal model, the tumor suppression rate could reach 100%.

Insights into a microwave susceptible agent for minimally invasive microwave tumor thermal therapy.

This work develops a kind of sodium alginate (SA) microcapsules as microwave susceptible agents for in vivo tumor microwave thermal therapy for the first time. Due to the excellent microwave susceptible properties and low bio-toxicity, excellent therapy efficiency can be achieved with the tumor inhibiting ratio of 97.85% after one-time microwave thermal therapy with ultralow power (1.8 W, 450 MHz). Meanwhile, the mechanism of high microwave heating efficiency was confirmed via computer-simulated model in theory, demonstrating that the spatial confinement efficiency of microcapsule walls endows the inside ions with high microwave susceptible properties. This strategy offers tremendous potential applications in clinical tumor treatment with the benefits of safety, reliability, effectiveness and minimally invasiveness.

A smart all-in-one theranostic platform for CT imaging guided tumor microwave thermotherapy based on IL@ZrO2 nanoparticles.

This study develops a simple hollow ZrO2 nanostructure as a carrier to encapsulate ionic liquid (IL), which integrates the CT imaging function of the ZrO2 shell and the microwave susceptibility function of the IL core. The simple nanostructure can be used as a multifunctional theranostic agent via combining diagnostic and therapeutic modalities into one "package". Based on the microwave susceptibility properties, the tumor inhibiting ratio can be over 90% in mice models after one-time thermal therapy upon microwave irradiation. In vitro and in vivo imaging results prove the potential of CT imaging application for real-time monitoring of biodistribution and metabolic processes, and assessing therapeutic outcomes. To our best knowledge, our study is the first example to achieve CT imaging and microwave thermal therapy simultaneously through a simple nanostructure. We anticipate that the simple IL@ZrO2 nanostructure may build a useful platform for the clinical imaging guided therapy of tumors.

Fast synthesis of fluorescent SiO2@CdTe nanoparticles with reusability in detection of H2O2.

In this study, highly fluorescent core/shell SiO2@CdTe nanoparticles (NPs) were synthesized conveniently and efficiently via a hydrothermal method. The as-prepared SiO2@CdTe NPs were uniform with good fluorescence preservation. The SiO2@CdTe NPs could be used for the rapid detection of H2O2 with good sensitivity within several minutes. Excellent linear relationships existed between the quenching degrees of the SiO2@CdTe NPs and the concentration of H2O2 in the range of 0.005 mM to 0.1 mM. The limit of detection (LOD) for H2O2 was 10 nM. Furthermore, it was proved that SiO2@CdTe NPs could be used repeatedly for H2O2 detection due to their easy separation, which is an important feature. The excellent performance of SiO2@CdTe NPs should facilitate their applications in chemistry or biology for detection of H2O2.

Green synthesis of Fe3O4 nanoparticles with controlled morphologies using urease and their application in dye adsorption.

This paper describes a simple and environmentally friendly method for the preparation of highly stable dispersions of Fe3O4 nanoparticles with controlled morphologies, in the same synthesis system for the first time. During the process, the Fe3O4 nanoparticles are formed using urease as a multifunctional reagent, including catalyst, template and dispersant, due to its enzymatic activity and special enzymatic steric structure. A possible formation mechanism for these Fe3O4 nanoparticles, which have various morphologies including nanospheres, nanosheets and nanorods, is proposed. The as-prepared nanoparticles show a larger specific surface area and a stronger magnetism, which enhances their dye adsorption capacity and increases their potential for application in wastewater treatment.

One-pot and one-step synthesis of bioactive urease/ZnFe2O4 nanocomposites and their application in detection of urea.

This communication describes a novel environmentally friendly method to prepare bioactive urease/ZnFe2O4 nanocomposites through a one-pot and one-step process. The synthetic procedure is triggered through a biological mineralization process of decomposition of urea catalyzed by urease. During the growth of ZnFe2O4, urease molecules are immobilized by original ZnFe2O4 nanoparticles. As a consequence, the bioactive urease/ZnFe2O4 nanoparticle composites are assembled. This simple route is expected to endow the bioactive nanocomposites with new properties for various interesting fields.

Preparation of electrophoretic nanoparticles for electronic paper.

As an electronic alternative for printed media, the E-paper has ultralow power consumption, reduced eyestrain, high contrast ratio. Electrophoretic displays are one of the most promising E-paper technologies, which are now widely used in consumer products. The properties of the electrophoretic display are mainly determined by the composition, size, light scattering properties, and density of the electrophoretic nanoparticles. First, we introduce the preparation of white and black electrophoretic nanoparticles, because the monochrome E-paper has achieved commercial success. Then the structure and properties of color electrophoretic nanoparticles for color E-paper products are discussed. In addition, the enhanced and novel electrophoretic nanoparticles are now propelling the development of next-generation E-paper with new applications. Finally, the active area of the preparation of electrophoretic nanoparticles is highlighted in terms of the development of future E-paper.

Ultrafast chemical aerosol flow synthesis of biocompatible fluorescent carbon dots for bioimaging.

A brand new method was successfully developed for the preparation of water-soluble carbon dots (CDs) through a chemical aerosol flow process. This is an ultrafast bottom-up strategy with the advantage of facile separation and purification and can be easily scaled up. In vitro and in vivo biotoxicity tests demonstrated that the CDs are highly biocompatible. Because of the high photostability and biocompatibility, the CDs are well suited for cellular imaging.

Fluorescent carbon dots for biolmaging and biosensing applications.

Due to the excellent biocompatibility, carbon dots (CDs), which are attracting considerable attention as new quantum-sized carbon-containing fluorescent nanoparticles, are becoming both an important class of imaging probes and a versatile platform for biosensors. In the process of transferring carbon dots from proof-of-concept studies to real bioimaging and biosensing applications, major advances have already been made in their synthesis, structure, properties, mechanism of fluorescence, and evaluation of biocompatibility and bio-applications. This review aims to summarize the recent developments and trends in carbon dots. Investigations of preparation methods, fluorescent properties and applications as biosensors and in bioimaging for carbon dots are described. In addition, this review highlights on the design and construct of a carbon dot fluorescent ratiometric biosensing platform for the detection of enzymatic activity, substrate and inhibitor concentrations related to the production or consumption of H2O2. This review provides perspectives on future opportunities and the remaining challenges confronting this research field.