Effects mechanism of bio-carrier filling rate on rotating biofilms and the reactor performance optimization method.

Benefited from the massive filling bio-carriers, the packed cage rotating biological contactors (RBCs) have better performance and application potentiality in wastewater treatment. Investigating the effects mechanism of bio-carrier filling rate is crucial for such reactors management. In this study, the pollutants removal performance, biofilms physical characteristics, and microbial communities of the biofilms under a series of bio-carrier filling rates were analyzed. The results shown, the pollutant removal rate and amount were quite different under different filling rates, and biofilms structure and microbial composition were the main factors affecting the pollutants removal performance. With the increasing filling rates, the biofilms were more mass increased (dry weight from 0.066 to 0.148 g/per carrier), thicker (from 340.30 to 850.84 µm) and lower dense (from 0.068 to 0.060 g/cm3). The microbial community composition of those biofilms was also quite different at the genus level. The effects mechanism of bio-carrier filling rate can be summarized: the filling rates affect the physical and biological characteristics of biofilms, which will further affect the microenvironment and microbial distribution in biofilms, and then determines the pollutant metabolic rate and metabolic pathway. This study will contribute to design better bio-carrier filling rate according to different wastewater treatment scenario, and promote the performance optimization of packed cage RBCs.

Molecular dynamic investigate the affection of EGFR by Tubemoside.

Tubemoside as a common traditional Chinese medicine is playing an important role in the field of prevention and treatment of lung cancer without any side effects. However, the reason and its mechanism remain unclear. In our study, the molecular dynamic simulation was used to investigate the mechanism at the molecular level. We found that the hydrogen bond network of proteins (three states of EGFR) was affected by Tubemoside. The movement and opening/closing state of protein was changed when combine with Tubemoside. The results of principal component analysis were used to prove the transform of proteins and the change of its movement. Electrostatic interactions of proteins also were studied. The numbers of active interaction sites will decrease while Tubemoside emerged in the protein, which will cause the activity change of EGFR for forming asymmetric dimers required for activation.