[Analysis of cerebral perfusion of leptomeningeal branch and perforating branch of unilateral middle cerebral artery with severe stenosis or occlusion based on multi-delay arterial spin labeling].

Objective: To compare the cerebral perfusion differences between the symptomatic patients and the asymptomatic patients with unilateral middle cerebral artery (MCA) severe stenosis or occlusion by using three post labeling delays (PLD) of the three-dimensional pseudo-continuous arterial spin labeling (pCASL) technique. Methods: The clinical characteristics and ASL data of the 27 patients with severe stenosis or occlusion of unilateral MCA (18 symptomatic, 9 asymptomatic) were prospectively enrolled from April 2018 to November 2019 in the Department of Radiology of China-Japan Friendship Hospital. There were 16 males and 11 females, age range from 29 to 85 (55±13) years. According to the symptoms, they were divided into symptomatic group (18 cases) and asymptomatic group (9 cases). The parameters of cerebral blood flow (CBF), mean cerebral blood flow (mCBF), arterial transit time (ATT) and arterial cerebral blood volume (aCBV) were obtained using the Cereflow software. One-way multivariate analysis of variance (one-way MANOVA) was used to compare the differences of cerebral perfusion parameters between symptomatic group and asymptomatic group, and between the affected side and the control side in the two groups. Two-way ANOVA was used to evaluate the effects of symptoms, hemisphere (affected and control side), PLD times (1.5, 2.0 and 2.5 s) and the interaction between the two factors. Results: The CBF of the affected leptomeningeal branch and perforating branch of MCA in symptomatic group was lower than that in asymptomatic group [(36.8±10.2) ml·100 g-1·min-1 versus (46.6±13.9) ml·100 g-1·min-1, F(1, 75)=13.279, P=0.000 49; (32.3±8.3) ml·100 g-1·min-1 versus (36.2±7.5) ml·100 g-1·min-1, F (1, 75)=4.065, P=0.047], and there was no interaction between the symptom and PLD [F(2, 75) =0.061, P=0.940]. In the symptomatic group, the CBF of the leptomeningeal branch and perforating branch of MCA in affected side was lower than that in control side [(36.8±10.2) ml·100 g-1·min-1 versus (43.7±10.0) ml·100 g-1·min-1, F(1, 102)=12.559, P=0.000 59; (32.3±8.3) ml·100 g-1·min-1 versus (36.4±8.0) ml·100 g-1·min-1, F(1, 102)=6.493, P=0.012]. In the symptomatic group, the CBF of leptomeningeal branch of MCA when PLD was 2.5 s was 7.34 ml·100 g-1·min-1, which were higher than that when PLD of 1.5 s (95%CI: 0.72-13.9, P=0.03). There was no interaction between PLD and hemisphere [F(2, 102) =0.307, P=0.736]. Conclusions: The collateral circulation in the blood supply area of MCA in asymptomatic patients with severe unilateral MCA stenosis or occlusion is more abundant than that in symptomatic patients. ASL can be an effective technique for evaluating the cerebral perfusion of collateral circulation in patients with severe stenosis or occlusion of MCA.

[Clinical features and imaging characteristics of Meniere's disease verified by intravenous gadolinium contrast-enhanced magnetic resonance imaging].

Objective: To analyze the clinical features and imaging characteristics of Meniere's disease (MD)verified by intravenous gadolinium contrast-enhanced magnetic resonance imaging(MRI). Methods: A total of 174 patients with Meniere's disease were involved in this study between March 2016 and October 2017. All of them underwent 3D-fluid attenuated inversion recovery (FLAIR) MRI and presented endolymphatic hydrops and clinical characteristics of suspected Meniere's disease. The clinical characteristics, the grades of endolymphatic hydrops (EH) using Nakashima grading standard were analyzed, and the correlation between grade and clinical features was evaluated. Results: There were totally 174 patients (88 males, 86 females), with the age of 7-83 years [mean age of (51.0±15.0)] years. Age of onset and disease course was 7-83 years [mean age of (44.6±16.6) years], 36.0 (12.0, 111.0) months (from 2 days to 70 years), respectively. EH was found in all the patients, and 169 cases (97.1%) manifested as modest or severe hydrops. Among the 174 patients, 139 cases (79.9%) presented unilateral EH, and 35 cases (20.1%) presented bilateral EH. The degree of EH in the latter was more serious than the former. For 174 patients, clinical characteristics were inconsistent, which was not in accord with the existing diagnostic criteria. Furthermore, there was a correlation between degree of hydrops and degree of hearing loss (P<0.05). Conclusions: EH in Meniere's disease can be measured objectively by intravenous gadolinium contrast-enhanced MRI. Clinical characteristics in MD verified by EH are not accordant with the existing criteria, which means the significance of EH in diagnosis of MD. Moreover, there's a correlation between degree and distribution of hydrops and degree of hearing loss.

[Research progress of mutational spectrum and pathophysiology of WFS1 gene in Wolfram syndrome and nonsyndromic low frequency sensorineural hearing loss].

Compound homozygous or heterozygous mutations in WFS1 can lead to autosomal recessive Wolfram syndrome (WS), and heterozygous mutations in WFS1 can lead to autosomal dominant non-syndromic low frequency sensorineural hearing loss (LFSNHL). In addition, mutations in the WFS region has relationship with diabetes and psychiatric diseases. In this paper, we provide an overview of genetic research with different phenotypes, including WS and LFSNHL.

Ginsenoside Rg3 attenuated omethoate-induced lung injury in rats.

Organophosphorus exposure affects different organs such as the lung, gastrointestinal tract, liver, and brain. The present experiment aimed to evaluate the effect of ginsenoside Rg3 on lung injury induced by acute omethoate poisoning. Rats were administered with omethoate subcutaneously at a single dose of 60 mg/kg, followed by ginsenoside Rg3 (5, 10, or 20 mg/kg) treatment. Histopathological examination of the lung was performed at 24 h after the omethoate exposure. The antioxidative parameters in the lung were also assayed. Moreover, the activities of acetylcholinesterase, myeloperoxidase, and the content of tumor necrosis factor α (TNF-α) in the lung were determined. The results showed that ginsenoside Rg3 attenuated omethoate-induced lung injury. Ginsenoside Rg3 increased the level of glutathione in the lung (p < 0.05 or p < 0.01). The altered activities of superoxide dismutase and catalase in the lung were also ameliorated by ginsenoside Rg3 treatment (p < 0.05 or p < 0.01). Ginsenoside Rg3 caused significant reductions in the contents of malondialdehyde, TNF-α, and the activity of myeloperoxidase (p < 0.05 or p < 0.01). The present study demonstrated that ginsenoside Rg3 had a protective effect against omethoate-induced lung injury in rats, and the mechanisms were related to its antioxidant potential and anti-inflammatory effect.

Osteopontin levels are elevated in patients with asthma.

Osteopontin (OPN) plays a role in T cell-mediated immunity, but its involvement in Th2-associated allergic responses, especially asthma, has not been investigated. This cross-sectional case-control study was designed to determine whether serum OPN levels are elevated in asthma patients and whether these correlated with disease severity. Serum samples were obtained from patients with mild (n = 42), moderate (n = 48) and severe (n = 39) asthma, and 41 healthy control subjects. OPN protein concentrations were measured by enzyme-linked immuno sorbent assay and were found to be significantly higher in all three asthma patient groups compared with healthy controls. There was no significant association between OPN concentration and disease severity. The data suggest a role for OPN in the pathogenesis of asthma. Further studies are needed to clarify the involvement of OPN in various aspects of asthma.

Free vascularized soft tissue flaps for coverage of the foot and ankle.

Microsurgical procedures for coverage of foot and ankle defects now are undertaken with a better understanding of biomechanical requirements, and concern for durability, aesthetics, donor site morbidity, and shoe fit. Well-contoured muscle flaps including the latissimus dorsi, rectus abdominis, and gracilis muscles frequently are used along with thin cutaneous flaps, especially from the lateral arm. When good local options are absent and exposed nerves, tendons, and bone, and surgical hardware are present, acute free flaps and delayed primary procedures are done frequently with excellent success rates (> 90%). Surgical adjuncts and pedorthic devices are used readily to enhance functional outcomes and to resolve shoe fitting and tissue breakdown problems. Free tissue transfers for foot and ankle coverage defects have reached state-of-the-art status, having been refined significantly to resolve many earlier problems.

[Treatment of myopic anisometropia by radial keratotomy].

18 cases of myopic anisometropia were treated by radial keratotomy (RK). The preoperative myopia in eyes with higher degrees of refractive error ranged from -3.25 to -11.00D (mean = -6.53D), the anisometropia ranged from -2.50 to -11.00D (mean = 4.95D) and the visual acuities of the naked eyes were 0.02 to 0.15 (mean = 0.08). A half year after the operation, on the average, there was a 0.46 increase in visual acuity, -4.57 D decrease in myopia and -3.60D decrease in anisometropia. Postoperatively, 14 cases had regained the binocular single vision and 6 cases had 60" stereoscopic vision which is in the normal range. Therefore, it is considered that RK operation not only can decrease myopic degree and increase visual acuity, but also can decrease or even eliminate anisometropia to facilitate the recovery of binocular single vision. In monocular or anisometropic myopia, if the corrected visual acuity of the myopic eye or the eye with higher degree of myopia in a case is not below 0.6, RK operation is indicated and if the anisometropia is lower than -9.00D, postoperatively, binocular single vision may be recovered. The preoperative examination of myopic anisometropia, the method of predicting prognosis and some attention points in the treatment are also mentioned in the paper.

Island skin flap with neurovascular pedicle from the dorsum of the index finger for reconstruction of the thumb.

This paper describes the successful reconstruction of badly injured thumbs in nineteen patients with transposition of a neurovascular pedicle island flap from the dorsum of the index finger. The flap has a consistent arterial supply (the first metacarpal artery), good sized veins, and terminal branches of the superficial radial nerve. Five patients had either palmar, dorsal, or circumferential loss of soft tissue from the thumb. Twelve patients had acute traumatic amputations requiring distal thumb reconstruction, and two patients had distal amputations with late reconstruction. In both immediate and late reconstruction, we maintained length and skin coverage with good circulation and sensation. This one-stage operation requires less postoperative care than many other procedures currently in use and may be employed in a wider variety of cases.

The use of a grommet bone liner for flexible hinge implant arthroplasty of the great toe.

Press-fit titanium grommets were developed to shield flexible hinged silicone implants used for arthroplasty of the radiocarpal, metacarpophalangeal, and metatarsophalangeal joints. Since 1985, 179 titanium circumferential grommets were used in 90 first metatarsophalangeal joints with excellent, pain-free, functional results and favorable bone response around the implant stems and at the bone-grommet interface. There were no complications due to particulate reactivity, implant fracture, or grommet fracture. The use of circumferential titanium grommets appears to be a safe and effective method to improve the long-term durability of flexible hinge implant arthroplasty of the first metatarsophalangeal joint.

Successful replantation in ten-digit complete amputations.

The authors report a case of successful replantation of all fingers in a ten-finger complete amputation. Among the factors responsible for the successful outcome were an adequate number of microsurgically-trained resident surgeons and staff, good organization and supervision by senior staff, emphasis on precision and perseverance in the operative procedures, and postoperative management. Follow-up is 15 months, and the patient now has a pair of functionally and cosmetically good hands.