Assuntos
Diabetes Mellitus Tipo 2/fisiopatologia , Estrogênios/deficiência , Fraturas do Fêmur/fisiopatologia , Consolidação da Fratura/fisiologia , Animais , Peso Corporal/fisiologia , Calo Ósseo/fisiopatologia , Colágeno Tipo I/metabolismo , Diabetes Mellitus Tipo 2/etiologia , Dieta Hiperlipídica , Modelos Animais de Doenças , Osteoporose/etiologia , Osteoporose/fisiopatologia , Ovariectomia , RatosRESUMO
The dysregulation of miR-532-5p is involved in the development of several cancers. Nevertheless, the roles of miR-532-5p in osteosarcoma (OS) have yet to be illuminated. In the present study, we found that miR-532-5p was significantly downregulated in both OS tissues and cell lines. The low level of miR-532-5p was associated with advance clinical stage and poor overall survival in patient with OS. The functional experiments implied that upregulation of miR-532-5p restrained OS U2OS cell growth and metastatic ability in vitro; induced apoptosis, and impaired OS cell growth in vivo. Mechanistically, chemokine (C-X-C Motif) ligand 2 (CXCL2) was proved as a target gene of miR-532-5p. The inhibitory effects of miR-532-5p on OS cell were rescued by CXCL2 overexpression. Altogether, we demonstrated that miR-532-5p exerted tumor-inhibitory functions in OS cell via regulating CXCL2.
