Clinicopathological analysis of nuclear protein in testis midline carcinoma / 中华病理学杂志

Objective: To investigate the clinicopathological features, immunophenotype and prognosis of nuclear protein in testis (NUT) midline carcinoma. Methods: Twenty-four resection cases of NUT midline carcinoma diagnosed at the Department of Pathology, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China from January 2018 to September 2022, were collected, and retrospectively analyzed for their clinicopathological characteristics. Relevant literature was reviewed. Results: All 24 cases of NUT midline carcinoma occurred in the chest or head and neck, including 14 men and 10 women, with a median age of 40 years. Histological examination showed that the tumors were poorly differentiated, with solid nested or sheet-like arrangement, small to medium-sized cells, sparse cytoplasm and coarse granular chromatin, including 5 cases with abrupt squamous epithelial differentiation. Immunohistochemistry showed that all 24 cases were positive for NUT protein, while 16 cases were p63 positive, 19 cases were p40 positive, 15 out of 18 cases were CK5/6 positive. Follow-up data were obtained for 21 patients (follow-up time range, 1-21 months), of which 11 survived, 10 died, and 3 were lost to follow-up. Conclusions: NUT midline carcinoma is a rare and highly aggressive malignancy with unique histological, immunophenotypic and molecular features. It has a poor prognosis.

Association between serum nitric oxide level and microalbuminuria:a 1 ∶ 2 matched case-control study / 中华流行病学杂志

Objective To investigate the association betweenserum nitric oxide and microalbuminuria.Methods Based on a community survey in Jinchang district Suzhou city,a 1 ∶ 2 matched case-control study was performed.A total of 208 cases with microalbuminuria were recruited.The controls were selected from the same community,with the same level of income.After matched with age,gender,waist circumference and fast plasma glucose,a total of 416 controls were selected.Values of serum nitric oxide were tested for all eligible participants.The association between serum nitric oxide level and microalbuminuria were analyzed by using the multivariate conditional logistic regression models.Results The mean level of serum nitric oxide was slightly lower for individuals with microalbuminuria (median,interquartile range:27.75,14.48-42.15 μmol/L) than those without (28.25,17.40-43.45 μ mol/L ).However,the difference was not statistically significant (P=0.316).Results from the multivariable conditional logistic regression model showed that serum nitric oxide level was not associated with microalbuminuria,after adjustment for all the confounders.Compared with the highest level of serum nitric oxide,the odds ratios of microalbuminuria for individuals in the 1 st,2nd and 3rd quartiles were not significantly different,after adjustment for confounders.In pairs with hypertension,the odds of microalbuminuria were 85％ higher for individuals with the lowest level of serum nitric oxide than those with the highest level (OR=1.85,95％ CI:0.96-3.57).Additionally,in pairs without hypertension,the odds of microalbuminuria was just 40％ higher for individuals with the lowest level of serum nitric oxide than those with the highest level (OR=1.40,95％CI:0.58-3.40).Conclusion There was no significant correlation between serum nitric oxide and microalbuminuria in the general population in our study.

Association between serum nitric oxide and hypertension among 1453 women in Suzhou / 中华流行病学杂志

Objective To investigate the association between serum nitric oxide (NO) and hypertension among women in Suzhou. Methods Blood pressure, height, weight and waist circumference (WC) were measured and factors including smoking, alcohol intake, family history of hypertension were investigated and blood glucose, blood lipid, serum NO were tested among 1453 women aged ≥30 years who lived in Jinchang district of Suzhou. Association between serum NO and hypertension was analyzed by univariate and multivariate methods. Results The mean levels of serum NO in hypertensive and normotensive persons were 28.17 (17.42-45.30)μmol/L and 27.56(17.19-44.42) μ mol/L, respectively, with no significant difference between the two groups (P ＞ 0.05).Results from multivariable logistic regression analysis showed that low serum NO was not associated with hypertension, after adjustment for confounders (OR=0.979, 95% CI: 0.747-1.283).The mean levels of systolic blood pressure / diastolic blood pressure were 130.1/83.3,128.5/82.7,129.8/83.2 and 129.1/83.3 mm Hg for whose serum NO level were in the first, second, third and fourth quartile,respectively. The risk of hypertension did not change along with the elevated serum NO levels.Compared to the first quartile of serum NO, the risks of hypertension in the second, third and fourth quartile did not change after adjustment for confounders and OR were 0.988 (0.709-1.377), 1.001(0.720-1.390) and 1.077 (0.774-1.499), respectively. Conclusion The serum NO level was not associated with hypertension in women in Suzhou.