[Establishment of simultaneous measurement method of 8 salivary components using urinary test paper and clinical evaluation of oral environment].

PURPOSE: Clinical findings were compared with glucose, protein, albumin, bilirubin, creatinine, pH, occult blood, ketone body, nitrite, and white blood cells contained in whole saliva to investigate the components that most markedly reflect the periodontal condition. MATERIAL AND METHOD: The subjects were staff of the Prosthodontics Department, Showa University, and patients who visited for dental treatments (57 subjects in total). At the first time, saliva samples were gargled with 1.5 ml of distilled water for 15 seconds and collected by spitting out into a paper cup. At the second time, saliva samples were collected by the same method. At the third time, saliva samples after chewing paraffin gum for 60 seconds were collected by spitting out into a paper cup. Thus whole saliva collecting that was divided on three times. After sampling, 8 mul of the saliva sample was dripped in reagent sticks for the 10 items of urinary test paper and the reflectance was measured using a specific reflectometer. In the periodontal tissue evaluation, the degree of alveolar bone resorption, probing value, and tooth mobility and the presence or absence of lesions in the root furcation were examined and classified into 4 ranks. The mean values in each periodontal disease rank and correlation between the periodontal disease ranks and the components were statistically analyzed. RESULTS: Bilirubin and ketone body were not measurable. The components density of the 8 items was increased as the periodontal disease rank increased. Regarding the correlation between the periodontal disease ranks and the components, high correlations were noted for protein, albumin, creatinine, pH, and white blood cells. CONCLUSION: The simultaneous measurement method of 8 salivary components using test paper may be very useful for the diagnosis of periodontal disease of abutment teeth.

Identification and characterization of the precursors committed to osteoclasts induced by TNF-related activation-induced cytokine/receptor activator of NF-kappa B ligand.

Osteoclasts are terminally differentiated from cells of monocyte/macrophage lineage by stimulation with TNF-related activation-induced cytokine (TRANCE) (receptor activator of NF-kappaB ligand/osteoprotegerin ligand/osteoclast differentiation factor/TNFSF11/CD254). In the present study, we attempted to determine when and how the cell fate of precursors becomes committed to osteoclasts following TRANCE stimulation. Although mouse bone marrow-derived macrophages (BMMs) were able to differentiate into either osteoclasts or dendritic cells, the cells no longer differentiated into dendritic cells after treatment with TRANCE for 24 h, indicating that their cell fate was committed to osteoclasts. Committed cells as well as BMMs were still quite weak in tartrate-resistant acid phosphatase activity, an osteoclast marker, and incorporated zymosan particles by phagocytosis. Interestingly, committed cells, but not BMMs, could still differentiate into osteoclasts even after incorporation of the zymosan particles. Furthermore, IL-4 and IFN-gamma, potent inhibitors of osteoclast differentiation, failed to inhibit osteoclast differentiation from committed cells, and blocking of TRANCE stimulation by osteoprotegerin resulted in cell death. Adhesion to culture plates was believed to be essential for osteoclast differentiation; however, committed cells, but not BMMs, differentiated into multinucleated osteoclasts without adhesion to culture plates. Although LPS activated the NF-kappaB-mediated pathway in BMMs as well as in committed cells, the mRNA expression level of TNF-alpha in the committed cells was significantly lower than that in BMMs. These results suggest that characteristics of the committed cells induced by TRANCE are distinctively different from that of BMMs and osteoclasts.

[Differences in whole salivary total protein concentration and protein fractions among the groups of dentulous subjects, edentulous subjects and periodontitis patients].

PURPOSE: To apply salivary proteins to the diagnosis of periodontal disease in partial denture supporting teeth, total protein concentrations and protein fractions in whole saliva were compared among dentulous subjects, edentulous subjects and periodontitis patients. MATERIALS AND METHODS: Eighty-five subjects/patients in total were studied, who consisted of 52 dentulous subjects with diagnosed normal periodontal tissue, 18 edentulous subjects using complete dentures and 15 patients with diagnosed periodontal disease. Total protein concentration in whole saliva was measured using dot blotting-silver staining, and protein fractions were analyzed using cellulose acetate membrane electrophoresis and silver staining. Based on the results obtained and some oral cavity examination items, the correlation between the periodontal pocket depth and the number of residual teeth was studied. RESULTS: Total protein concentration was highest in the group of periodontal disease patients and significantly different from that in the group of dentulous subjects (p<0.01). The percentage area and concentrations of albumin fraction were largest in periodontal patients, followed by dentulous subjects and edentulous subjects in this order. A significant difference (p<0.01) was observed in albumin concentration between periodontal patients and edentulous or dentulous subjects. Concentrations of immunoglobulin A and Gamma-globulin were significantly higher in periodontal patients than in dentulous subjects (p<0.01). In addition, among oral cavity examination items, the total periodontal pocket depth, the number of residual teeth and the number of teeth with periodontal pocket depth of over 4 mm were significantly correlated with the percentage area of albumin fraction or albumin concentration. CONCLUSIONS: Albumin concentration in whole saliva was suggested to be most effective for diagnosing periodontal disease in supporting teeth.