Molecular cloning, expression, mRNA secondary structure and immunological characterization of mussel foot proteins (Mfps) (Mollusca: Bivalvia).

The macroscale production of mussel foot proteins (Mfps) in the expression system has not succeeded to date. The principal reasons for this are low levels of expression and yield of Mfps, lack of post-translational modifications (PTMs), and immunological toxic effects on the host system. Identification of post-translational modification sites, suitable expression hosts, and immunological responses through an experimental approach is very costly and time-consuming. However, in the present study, in silico post-translation modification, antigenicity, allergenicity, and the immunological reaction of all available Mfps were characterized. Furthermore, all Mfps were codon optimized in three different expression systems to determine the best expression host. Finally, we performed the in-silico cloning of all codon-optimized Mfps in a suitable host (E. coli K12, pET28a(+) vector) and analyzed the secondary structure of mRNA and its structural stability. Among the 78 Mfps, six fps are considered potential allergenic proteins, six fps are considered non-allergenic proteins, and all other fps are probably allergenic. High antigenicity was observed in bacterial cells as compared to yeast and tumor cells. Nevertheless, the predicted expression of Mfps in a bacterial host is higher than in other expression hosts. Important to note that all Mfps showed significant immunological activity in the human system, and we concluded that these antigenic, allergenic, and immunological properties are directly correlated with their amino acid composition. The study's major goal is to provide a comprehensive understanding of Mfps and aid in the future genetic engineering and expression of Mfps and its diverse applications in different fields.Communicated by Ramaswamy H. Sarma.

Dye and metal ion adsorption ability of Asian green mussel byssus thread complex; their microscopic and thermal property characterization.

Various physical, chemical, and biological factors act as pollutants and cause deleterious effects on the environment and living organisms. Several researchers are developing eco-friendly good adsorbent from agriculture waste materials for pollutant removal, especially aromatic dyes and heavy metal removal. We used the Asian green mussel byssus thread, a natural mariculture waste product for the removal of aromatic dyes (methylene blue and eosin Y) and heavy metals (zinc, copper, iron, mercury and lead). We documented the dynamicity of byssus thread dye and metal removal at different pH (pH 2-10) and different concentrations. The highest amount of metal removal was observed at pH 6.0, and the dye removal efficacy is related to the property of the dye (i.e. anionic or cationic dye). The byssus thread had a natural fluorescent property upon UV-excitation; however, microscopic examination revealed that metal and dye coordination significantly alter the byssus plaque region's physical and chemical property rather than the thread regions. It was further confirmed by using DSC and TGA characterization of de-metaled and metal-treated byssus thread complex. We concluded that Perna viridis byssus thread could be used as a strong adsorbent for dye and metal removal from the water.