RESUMO
In order to clarify the action of the bcr-abl, a growth factor dependent human leukemic cell line (HSM-911) was transfected with p210bcr-abl or bcr-v-abl by electroporation. The cells transfected with bcr-v-abl, but not the cells transfected with p210bcr-abl, became growth factor independent. Some clones of the cells transfected with p210bcr-abl demonstrated cellular maturation (nuclear segmentation, becoming positive for naphthol ASD chloroacetate esterase, the disappearance of CD34 expression and the appearance of glycophorin A and CD10 expression). Moreover, these clones transfected with p210bcr-abl demonstrated apoptosis (increased expression of Fas and DNA ladder formation suggesting apoptotic DNA fragmentation). These findings demonstrated the different actions of p210 bcr-abl and bcr-v-abl, the former of which gave the cells the characteristics of maturation like the cells from chronic myelogenous leukemia, and the latter of which rendered the cells grow autonomously.
Assuntos
Proteínas de Fusão bcr-abl/genética , Leucemia Aguda Bifenotípica/genética , Leucemia Aguda Bifenotípica/patologia , Apoptose/fisiologia , Divisão Celular/fisiologia , Proteínas de Fusão bcr-abl/farmacologia , Humanos , Transfecção , Células Tumorais CultivadasRESUMO
Prolonged hyperimmmunization of C3H mice with casein resulted in immune deposits in glomeruli which led to subsequent amyloid deposition. During the immunization, the animals were in state of extreme antigen excess without any detectable circulating antibodies. The immunofluorescent findings in the later period revealed predominant IgM deposition in glomeruli, which was quite consistent with the dominant presence of IgM antibody-forming cells in spleen in the apparently tolerant state.
