Suppression of fibroblast and bacterial adhesion by MPC coating on acrylic intraocular lenses.

PURPOSE: To investigate cell adhesion to intraocular lens (IOL) surfaces having different properties using bacteria and fibroblasts. SETTING: Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan. METHODS: The polished acrylic IOLs VA60-BB and VA-60-CB (Hoya) were used with no coating or after coating with poly(2-methacryloyloxyethyl phosphocholine-co-n-butyl-methacrylate) (MPC) or methane gas plasma. Each IOL was placed in a culture of Staphylococcus aureus (5 x 10(7) colony forming units [CFU]/mL) or fibroblasts (SUSM-1, 1 x 10(4) cells/mL), and the cells adhering to the IOL surface were counted after the culture. Fibroblast adhesiveness was evaluated by centrifuging post-culture IOLs in test tubes at up to 15,000 rpm and looking at the ICAM-1 mRNA expression in the adhering fibroblasts using real-time polymerase chain reaction. RESULTS: After 1 minute in the bacterial culture, the mean adhering bacteria count was 0.7 x 10(6) CFU in the MPC-coated IOL group and about 2.0 x 106 CFU in the noncoated IOL group (P = 0.03) and the plasma-coated IOL group (P = 0.02). After 96 hours in the fibroblast culture, the adhering fibroblast count was 2.2/mm2 in the MPC-coated IOL group, significantly lower (P = 0.009) than 57.6/mm2 in the noncoated IOL group and 125.8/mm2 in the plasma-coated IOL group. Cell adhesion and ICAM-1 mRNA expression were weak on the MPC-coated IOL, intermediate on the noncoated IOL, and relatively strong on the plasma-coated IOL. CONCLUSION: The MPC-coated IOL surface inhibited bacterial and fibroblast adhesion, which is an initial stage of cell proliferation and expression, suggesting that MPC coating may provide an effective means of reducing the risk for endophthalimitis in IOL implantation.

Cell adhesion to acrylic intraocular lens associated with lens surface properties.

PURPOSE: To examine the number cells adhering to acrylic intraocular lenses (IOLs) having different degrees of surface roughness and acrylic IOLs having different contact angles. SETTING: Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan. METHODS: In this study, VA-60 CB acrylic IOLs were used. After different durations of polishing of the optics, the roughness values of the lenses were set at 10.0 nm, 2.5 nm, and 0.7 nm. Intraocular lenses were also chemically coated to achieve angles of contact of 100 degrees and 57.5 degrees, with untreated IOLs having a 93.5-degree angle of contact. Intraocular lenses were cultured for 96 hours in 1 x 10(7) spleen cells/mL of developed experimental autoimmune uveoretinitis, and the number of cells adhering to the IOLs was counted. RESULTS: Fewer cells adhered to the optics of IOLs with lower roughness values, and fewer cells adhered to the IOL surface with increased contact angles. CONCLUSION: Hydrophobic optics with small roughness values of acrylic IOLs may reduce the number of adherent cells and escaped the unexpected inflammatory cell reaction associated with intraocular inflammation in the eye.

Surface roughness of intraocular lenses and inflammatory cell adhesion to lens surfaces.

PURPOSE: To assess and examine the relationship between the degree of intraocular lens (IOL) surface roughness and the severity of inflammatory cell adhesion to the optical surface. SETTING: Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan. METHODS: Poly(methyl methacrylate) IOLs with 3 degrees of mean surface roughness (Ra) were used including IOLs with an unpolished optical surface and IOLs with optical surfaces polished for 1 day or 5 days. The IOLs were cultured for 96 hours with 1 cell/mL x 10(7) cells/mL of splenocytes from rats that developed experimental autoimmune uveoretinitis (EAU). At the end of culture, the number of inflammatory cells adhering to the optical surfaces of the IOLs was counted. RESULTS: The mean Ra for the unpolished IOLs, IOLs polished for 1 day, and IOLs polished for 5 days was 14.17 nm, 3.71 nm, and 1.37 nm, respectively. The mean numbers of cells that adhered to the optical surfaces of the IOLs after culturing with splenocytes from rats with EAU were 86 cells/mm(2), 66 cells/mm(2), and 48 cells/mm(2), respectively. There was a significant difference between the unpolished IOLs and the IOLs polished for 5 days (P =.003, Mann-Whitney U test). CONCLUSION: Inflammatory cell adhesion to the IOL optical surface was affected by the Ra value of the IOL.