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AIM:To determine the effects of Tongxinluo (TXL) on connexin 43 (Cx43) remodeling and ven-tricular arrhythmia ( VA) after myocardial infarction ( MI) in rats .METHODS:Male SD rats were randomly divided into sham-operated (sham) group (n=25) and operation group (n=75).The left anterior descending (LAD) was ligated in operated group , while the rats in sham group only underwent pericardiotomy .The rats in operation group which survived for 3 d after operation were randomly assigned to TXL group and MI group .The rats in TXL group was administrated with TXL (2 g? kg-1? d-1, intragastric administration) for 4 weeks, while normal saline was applied to the rats in sham group and MI group.The levels of interleukin-1β(IL-1β) and endothelin-1 (ET-1) in the tissue from the border zone were measured by ELISA after treatment .The distribution and the mRNA and protein expression of Cx 43 were detected by immunohisto-chemical staining , RT-PCR and Western blotting , respectively .The burst pacing was used to induce ventricular arrhythmia ( VA) .RESULTS:Compared with sham group , the levels of IL-1βand ET-1 and the incidence of VA were significantly increased , while the mRNA and protein expression of Cx 43 was markedly reduced with irregular distribution in MI group (P<0.05).Compared with MI group, the levels of IL-1βand ET-1 and the incidence of VA were significantly reduced , while the expression of Cx 43 at mRNA and protein levels was markedly increased with augmented linear distribution in the myocardial cell intercalated disc in TXL group (P<0.05).CONCLUSION:TXL reduces the incidence of VA after MI via inhibiting the Cx43 remodeling .
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[ABSTRACT]AIM:TodeterminetheeffectsofShensongyangxincapsule(SSYX)ontheventricularelectrical properties, structural remodeling and cardiac function in the rats with diabetes mellitus (DM).METHODS:Male SD rats (n=45) were randomly divided into control group (n=15), DM group (n=15) and SSYX group (n=15).The rats in DM group and SSYX group were injected with streptozotocin (60 mg/kg, ip), while the rats in control group were given normal saline (1 mL/kg, ip).The blood samples were collected 72 h after treatment for determining the blood glucose lev-els in DM group and SSYX group .The model rats in SSYX group were administered with SSYX (1 g· kg-1 · d-1 , ig) for 6 weeks, while the other rats received normal saline (2 mL· kg-1· d-1, ig).The echocardiography was used to assess the cardiac function , and the lead II electrocardiogram was also recorded in all the animals .The radioimmunoassay and Masson trichrome staining were used to measure the plasma levels of endothelin -1 (ET-1) and the collagen deposition in the ventricles, respectively.A whole Langendorff-perfused heart model was used to conduct the electrophysiologic study .The monophasic action potential ( MAP) and the ventricular effective refractory period ( VERP) were recorded in the left anteri-or free wall ( LAF) , and the burst pacing was used to induce ventricular arrhythmia ( VA) .RESULTS: Compared with control group, the VERP, action potential duration (APD), QT interval, incidence of VA, degree of myocardial fibrosis and plasma level of ET-1 were increased , while the cardiac function was declined in DM group .Compared with DM group , the VERP, APD, QT interval, incidence of VA, degree of myocardial fibrosis and plasma level of ET-1 were all de-creased, while the cardiac function was improved in SSYX group .CONCLUSION: SSYX attenuates the electrical and structural remodeling and improves the cardiac function in DM rats .
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Objective To evaluate the therapeutic effetive,toxicity and survial rate of non-operation esophageal carcinoma patients treated by tegafur plus cisplatin combined with three-dimensional conformal radiotherapy (3D-CRT).Methods 184 cases of non-operation patients with esophageal carcinoma were divided into two groups.The cases of treatment group were treated by cisplatin and tegafur.The cases of control group were treated by cisplatin and 5-Fu.All patients received 3D-CRT,and the total radiation dose was 50-70 Gy.Each group had 14 cases treated with primary chemotherapy for 1 course,28 cases for 2 courses,and 50 cases without any chemotherapy.Results The recent effect rate was 69.57 ％ (64/92) in treatment group and 67.39 ％ (62/92) in control group,there was no statistical difference (x2 =0.101,P =0.874).Radioactive esophagitis and leukopenia were the main side effect of treatment.The treatment group was significantly lower than that of the control group,the difference had statistical significance.The 1-,2-and 3-year survival rates were 74.9 ％,61.6 ％,52.8 ％,respectively in treatment group and 75.8 ％,56.9 ％,48.0 ％,respectively in control group.The control group survival rates were significantly higher than the treatment group,the difference had statistical significance (x2 =7.325,P =0.007).Conclusions Cisplatin plus tegafur or 5-Fu combined with 3D-CRT is effective way of treatment for non-operation of esophageal carcinoma.The toxicity of cisplatin plus tegafur group is significantly lower than cisplatin plus 5-Fu group,therefor it can improve the quality of life of the patients obviously.
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Objective To investigate the significance and mechanism of intracerebroventricular injection viper venom nerve growth factor (Vngf) in rat neural plasticity after cerebral ischemia reperfusion injury.Methods Ninety Wistar male rats were randomly assigned into Vngf-25 U group (n = 18), Vngf-50 U group (n = 18), Vngf-100 U group (n = 18), ischemia reperfusion group (n = 18) and sham operated group.The expression of candidate plasticity-related gene 15(cpg-15) Mrna and nuclear factor of kappa B ( NF-Κb ) Mrna in rat brain tissues which were collection at 2,7,14 days after surgery were evaluated by the real time PCR.Results The expression of cpg-15 Mrna and NF-Κb Mrna began to increase after surgery( the F value of cpg-15:70.43, 34.11, 31.89, the F value of NF-Κb: 27.47, 34.56, 31.89,P＜0.01).At the same time, expression of cpg-15 Mrna and NF-Κb Mrna in the Vngf groups was significantly different from the I/R group and the sham operated group (the F value of cpg-15:48.18, 55.93, 78.43, the F value of NF-Κb: 45.92, 55.72, 50.49, P ＜0.01).The more Vngf were injected, the more cpg-15 Mrna and NF-Κb Mrna were expressed in Vngf groups.Conclusions The Vngf could accelerate neural plasticity and restore neurofunctional defect through up-regulated the expression of cpg-15 and NF-Κb.
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@# Objective To investigate the cerebral ischemia/reperfusion protection mechanism of viper venom nerve growth factor(vNGF) by the change of expression of growth associated protein-43 (GAP-43) and neurological function.Methods 45 adult male Wistar rats (weight 220～280 g) were divided randomly into 3 groups: sham group(S, n=9), balanced salt solution group (BSS, n=9) and venom nerve growth factor group (vNGF, n=27). Each group was observed for 7 days. vNGF group was divided into 25 U, 50 U and 100 U subgroups respectively. The following indexes in 3 groups were observed respectively: neurologic deficits and the expression of GAP-43 (immunohistochemistry method).Results Neurological function: The scores of neurological function was 0 in S group. The neurological deficits score was lower at the same time in vNGF group than that in BSS group (P<0.05). Immunohistochemistry: GAP-43 expressed in both BSS group and vNGF group. The expression of GAP-43 in vNGF group increased in 25 U, and to maximum in 100 U. The expression of GAP-43 in BSS group was significantly lower than in vNGF group (P<0.05). Conclusion vNGF can effectively enhance and prolong the expression of GAP-43, increase the survival rats of nerve cells, and has the protection effect on nerve cells after cerebral ischemia injured.
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Objective: To compare the cognition of cancer patients and their relatives,so as to provide references for cognitive intervention.Methods: Cognition questionnaire consists 19 questions to which most patients and their relatives concern.Sixty new cases of cancer patients and their relatives were included in this study.Results: Answers to the 5 following questions from the patients and their relatives were different with statistical significance: do you know the diagnosis(83% patients know,97% relatives know,?2=5.93,P=0.015);whether to inform patients of diagnosis or not(87% patients chose yes,65% relatives chose yes,?2=7.69,P=0.006);is it necessary to tell patient intimate therapeutic regimen(77% patients thought it is necessary,62% relatives thought it is necessary,?2=8.71,P=0.013);who decide the method(s) of treatment(7% patients and 17% relatives' answer is the patients,?2=7.19,P=0.028);if the treatment is unable to cure your disease,what would be your option(80% patients and 97% relatives chose treatment,?2=8.09,P=0.004).Different educational background(?2=5.63,P=0.018) and occupation(?2=4.10,P=0.043) affected their cognition on being informed of the intimate therapeutic regimen.Conclusion:Patients` and relatives` cognition of being informed of the diagnosis and therapy are diverse from each other.In communication with them,doctors must treat them respectively.
