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1.
Cytogenet Genome Res ; 108(1-3): 83-90, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-15545719

RESUMO

Chromosome rearrangement has been considered to be important in the evolutionary process. Here, we demonstrate the evolutionary relationship of the rearranged human chromosome 12 and the corresponding chromosome XII in apes (chimpanzee, bonobo, gorilla, orangutan, and gibbon) by examining PCR products derived from the breakpoints of inversions and by conducting shotgun sequencing of a gorilla fosmid clone containing the breakpoint and a "duplicated segment" (duplicon). We confirmed that a pair of 23-kb duplicons flank the breakpoints of inversions on the long and short arms of chimpanzee chromosome XII. Although only the 23-kb duplicon on the long arm of chimpanzee chromosome XII and its telomeric flanking sequence are found to be conserved among the hominoids (human, great apes, and gibbons), the duplicon on the short arm of chimpanzee chromosome XII is suggested to be the result of a duplication from that on the long arm. Furthermore, the shotgun sequencing of a gorilla fosmid indicated that the breakpoint on the long arm of the gorilla is located at a different position 1.9 kb from that of chimpanzee. The region is flanked by a sequence homologous to that of human chromosome 6q22. Our findings and sequence analysis suggest a close relationship between segmental duplication and chromosome rearrangement (or breakpoint of inversion) in Hominoidea. The role of the chromosome rearrangement in speciation is also discussed based on our new results.


Assuntos
Sequência de Bases/genética , Cromossomos Humanos Par 12/genética , Rearranjo Gênico/genética , Hominidae/genética , Nucleotídeos/genética , Animais , Quebra Cromossômica/genética , Cromossomos Artificiais Bacterianos/genética , Cromossomos de Mamíferos/genética , Clonagem Molecular , Gorilla gorilla/genética , Humanos , Dados de Sequência Molecular , Pan troglodytes/genética , Reação em Cadeia da Polimerase/métodos , Pongo pygmaeus/genética , Análise de Sequência de DNA/métodos
2.
J Hered ; 93(1): 9-18, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12011169

RESUMO

Cercopithecus aethiops can be classified into four subspecies by morphology and by geographic distribution. However, the phylogenetic relationship between these subspecies is unclear. We previously found five distinct haplogroups of mitochondrial DNA (mtDNA) in the subspecies C. aethiops aethiops at the restriction fragment length polymorphism (RFLP) level, and found that those haplogroups are parapatrically distributed in their habitat. To determine the relationship between subspeciation and haplogroup formation in a subspecies, we compared mtDNA control region and 12S rRNA gene sequences (approximately 700 bp) in C. a. aethiops, two other subspecies of C. aethiops, and two species of Cercopithecus: The diversity between haplogroups in C. a. aethiops was almost the same as that between subspecies. This similar level of diversification between and within haplogroups may explain why a previously obtained mtDNA tree did not show monophyletic branching according to subspecies.


Assuntos
Chlorocebus aethiops/genética , DNA Mitocondrial , Evolução Molecular , Variação Genética , Animais , Sequência de Bases , Chlorocebus aethiops/classificação , Genes de RNAr , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência
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