Quantitative analysis of CD3ε in a cloned canine lymphoma cell line by selected reaction monitoring assay.

We established a mass spectrometry-based quantitative method of assaying CD3ε, a component of the T-cell receptor complex. It revealed a CD3ε level of 1 mol per cell in a newly derived canine T-cell lymphoma cell line. Our results suggest that this method has sufficient sensitivity to quantify CD3ε levels in canine lymphoma cells reliably.

[Side effects of opioid and gene variants].

There are individual differences in the analgesic effect and the side effect of the opioid to which genetic variation may be related. Analysis of micro-opioid receptor knockout mice indicated that inhibition of gastrointestinal transit by morphine is mediated by micro-opioid receptor. Our study suggested that gastrointestinal symptom (especially loss of appetite) as a side effect of opioid could be associated with the gene polymorphism of dopamine D2 receptor. Understanding of the relationships between gene polymorphisms and opioid sensitivities may lead to more-accurate prediction of the opioid sensitivity and opioid requirements in individual patients.