1.
Anal Biochem
; 128(1): 223-6, 1983 Jan.
Artigo
em Inglês
| MEDLINE
| ID: mdl-6221672
RESUMO
The electrophoretic mobilities of DNA polymer fragments in an agarose gel have been measured from a photograph of the gel by different methods and converted to lengths by the reciprocal method. The method of measurement can introduce large errors in the length estimates. The use of a digital microdensitometer to obtain optical density profiles of gel tracks with subsequent computer processing to find peak positions was found to give the most accurate DNA lengths.