Distribution of DNA, proteins, and lipids in cells of the olfactory bulb in rats of different ages.

Luminescence and absorption stains specific for DNA (acridine orange, ethidium bromide), proteins (silver nitrate), and lipids (Sudan III) were used to study the distribution of DNA, proteins, and lipids in sections of the olfactory bulb in rats, studies being performed after fixation of brains with paraformaldehyde. DNA was found to be more abundant in the glomerular cell layer than the mitral cell layer. Higher quantities of DNA were present in the granular layer, located beneath the mitral layer. The characteristics of cell layers in the olfactory bulb were studied in rats aged two days and one month. There were differences between the layers of rats of different ages in terms of the content and distribution of DNA, though there were no differences in the total protein or lipid contents. Glomeruli were immature in two-day-old rats.

[DNA, proteins and lipids distribution in the cells of olfactory bulbs of rats of various ages].

Using luminescent and absorption stains, specific for DNA (acridine orange, ethidium bromide), proteins (silver nitrate) and lipids (sudan III), the distribution of these substances was studied in the sections of rat olfactory bulbs, fixed by paraformaldehyde. DNA prevalence was found in glomerular cell layer as compared with the mitral one. Large amount of DNA was detected in granular cell layer, underlying the mitral one. The peculiarities of cellular layers of olfactory bulbs of 2-day-old rats were compared with those ones in 1-month-old animals. In rats of different ages, the differences were found in DNA content and distribution between layers, while no differences were detected in total protein and lipids. In 2-day-old rats glomerular underdevelopment was demonstrated.

[Growth of bacteria degrading naphthalene and salicylate at low temperatures]. / Rost bakterii-destruktorov naftalina i salitsilata pri ponizhennykh temperaturakh.

A total of 58 bacterial strains degrading naphthalene and salicylate were isolated from soil samples polluted with oil products, collected in different regions of Russia during winter and summer. The isolates were assessed for their ability to grow at low temperatures (4, 8, and 15 degrees C); bacteria growing at 4 degrees C in the presence of naphthalene or salicylate accounted for 65% and 53%, respectively, of the strains isolated. The strains differed in the temperature dependence of their growth rates. It was demonstrated that the type of expression of Nah+ phenotype at low temperatures depended on the combination of the host bacterium and the plasmid.