RESUMO
The authors have developed a panel of monoclonal antibodies (MCA) of IPO series (Institute of Problems of Oncology, Kiev). RPMI-1788 cell line, Daudi, as well as splenocytes of a patient with hairy-cell leukemia were used for immunization of mice. It has been shown that IPO-3, IPO-10 and IPO-24 MCA are directed against differentiation antigens of human B-lymphocytes. IPO-4 MCA reveal the antigen of activated T- and B-cells. IPO-5 and IPO-20 MCA recognize HLA-ABC, while IPO-37 a common leucocytic antigen. IPO-38 MCA are directed against the nuclear antigen. The possibilities of using IPO MCA in hematology have been discussed.
Assuntos
Anticorpos Monoclonais , Leucemia/diagnóstico , Linfoma/diagnóstico , Academias e Institutos , Animais , Anticorpos Monoclonais/biossíntese , Anticorpos Monoclonais/imunologia , Antígenos CD/imunologia , Diagnóstico Diferencial , Humanos , Leucemia/imunologia , Linfócitos/imunologia , Linfoma/imunologia , Oncologia , Camundongos , UcrâniaRESUMO
Spleen cells from BALB/c mice previously immunized with B-lymphoblastoid cell line RPMI-1788 were fused with P3-X-63-Ag8.653 myeloma cells. Monoclonal antibodies (Ab) IPO-4 were screened on 18 cell lines by the indirect immuno-fluorescence method. Cryostat sections of tissues were stained according to the PAP technique. The Mab IPO-4 were tested for reactivity with blood cells of 17 healthy persons and 102 patients with chronic lymphocytic leukemia, acute lymphoblastic and myeloblastic leukemias, hairy cell leukemia, multiple myeloma, non-Hodgkin's lymphomas and Hodgkin's disease and mitogen stimulated lymphocytes. MAb IPO-4 were found to be directed against antigen expressed on activated T and B cells.
Assuntos
Anticorpos Monoclonais , Antígenos de Diferenciação de Linfócitos B/imunologia , Antígenos de Diferenciação de Linfócitos T/imunologia , Linfócitos B/imunologia , Linfócitos T/imunologia , Animais , Biomarcadores Tumorais , Linhagem Celular , Humanos , Leucemia/diagnóstico , Leucemia/imunologia , Ativação Linfocitária , Linfoma/diagnóstico , Linfoma/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Mieloma Múltiplo/diagnóstico , Mieloma Múltiplo/imunologiaRESUMO
Monoclonal antibodies IPO-10 were generated following immunization of a BALB/c mice with human cell line Daudi. Reactivity of this mABs was studied by indirect immunofluorescence technique with 10 human cell lines, blood cells of healthy persons and of patients with the malignant lymphoproliferative diseases. Studies on normal and neoplastic B cell suggest that mABs IPO-10 recognizing antigen is B lineage restricted. The antigen defined by the mAb IPO-10 appear to include most but not all stages of B cell differentiation.
Assuntos
Anticorpos Monoclonais/isolamento & purificação , Antígenos de Diferenciação de Linfócitos B/imunologia , Animais , Anticorpos Monoclonais/análise , Linfócitos B/imunologia , Epitopos/análise , Epitopos/isolamento & purificação , Imunofluorescência , Antígenos de Histocompatibilidade Classe II/imunologia , Humanos , Hibridomas/imunologia , Imunização/métodos , Transtornos Linfoproliferativos/imunologia , Camundongos , Camundongos Endogâmicos BALB CRESUMO
Monoclonal antibodies IPO-1--IPO-8 against surface antigens of B-lymphoblastoid cell line RPMI-1788 were prepared. Murine hybridomas were obtained by fusion of immune spleen cells and myeloma cells. Screening of specific antibody production was carried out by indirect immunofluorescence. Expression of these antibodies against a panel of 11 human cell lines was carried out by indirect immunofluorescence. Expression of antigens detected with IPO-1--IPO-8 were investigated on peripheral blood cells of healthy individuals and patients with CLL, ALL, myeloma and on lymph node cells of patients with Hodgkin's disease. Specificity of these MoAbs is discussed. IPO-5 is shown to react with the HLA-related determinant. The antibody IPO-3 appears to recognize a differentiation antigen of human B cells.
