[Effect of irradiation of blood with a helium-neon laser on aggregation of neutrophilic granulocytes of patients with ischemic heart disease]. / Vliianie oblucheniia krovi gelii-neonovym lazerom na agregatsiiu neitrofil'nykh granulotsitov bol'nykh ishemicheskoi bolezn'iu serdtsa.

Blood samples from 30 patients with myocardial ischemia and 25 healthy donors were irradiated with He-Ne laser. ATP release was measured by the luciferin-luciferase test system on a PICA lumo-aggregometer (Chrono-Log Corporation, USA). There was no correlation between lectin-induced ATP release and cell aggregation. Neutrophils obtained from donors and patients differed in the number of lectin receptors, including mannose and sialic acids on the cellular surface. Following laser irradiation, WGA- and SBA-induced aggregation was decreased, while Con-A-induced aggregation was increased.

[Lectin-induced aggregation of neutrophilic granulocytes before and after irradiation of the blood with a helium-neon laser]. / Lektinindutsirovannaia agregatsiia neitrofil'nykh granulotsitov do i posle oblucheniia krovi gelii neonovym laserom.

To clarify the changes in cellular reactivity in response to single irradiation of blood with He-Ne laser, the authors studied neutrophil aggregation and neutrophil ATP release induced by stimulation with lectins diverse in carbohydrate specificity. Blood samples from 30 healthy donors were investigated using PICA lumoaggregometer (Chrono-Log Corporation, USA) and concanavalin A, PHA-L, WGA, SBA as aggregation inductors. He-Ne laser radiation in most cases results in reduced neutrophil capacity for aggregation. Nevertheless, it activates ATP release under stimulation with lectins of various carbohydrate specificity. It follows that mechanisms of lectin-induced reactions, aggregation of neutrophils and ATP release, are different.

[Lectin-induced chemiluminescence of peripheral blood neutrophils in patients with ischemic heart disease before and after blood irradiation with helium-neon laser]. / Lektinindutsirovannaia khemiliuminestsentsiia neitrofil'nykhgranu- lotsitov perifericheskoi krovi bol'nykh ishemicheskoi bolezn'iu serdtsa do i posle oblucheniia krovi gelii-neonovym lazerom.

The blood taken from 35 patients with coronary heart disease and 30 healthy donors was irradiated with He-Ne laser, which resulted in a decrease in its count of segmented neutrophilic granulocytes. Lectins bound to various carbohydrate determinants onto the neutrophil surface were shown to affect changes occurring after luminol-depended chemiluminescence irradiation in patients and healthy persons in different ways. The patients' neutrophils contained lower levels of radiommunologically detectable leukotriene B4. Thromboxane B2 levels also dropped following the irradiation. The laser irradiation induced elimination of some less resistant cells from blood flow, i.e. "rejuvenation" of a cell population of neutrophilic granulocytes. The remaining cells differed in the composition and reactivity of surface lectin receptors and in the content of biologically active substances, which is likely to play the key role in the mechanism responsible for the therapeutical effect of He-Ne laser.

[Changes in lectin-induced chemiluminescence of neutrophilic granulocytes after irradiation of blood with helium-neon lasers]. / Izmenenie lektinindutsirovannoi khemiliuminestsentsii neitrofil'nykh granulotsitov posle oblucheniia krovi svetom gelii-neonovogo lazera.

Blood obtained from 33 healthy donors was irradiated with He-Ne laser. During this process the count of segmented neutrophils decreased, while the content of radioimmunologically determined leukotriene B4 and thromboxane B2 increased in the other neutrophils. Application of mannose-specific lectins (Con A, PSL) as inductors caused the increase, while application of other carbohydrate-specific lectins caused the decrease of luminol-dependent chemiluminescence in neutrophilic granulocyte suspension after the irradiation. As a result of direct laser effect on the blood cells some portion of the less resistant cells were eliminated from the blood flow, i.e. "rejuvenation" of the neutrophilic granulocyte population in the cells takes place.

[Serological varieties of Klebsiella pneumoniae in pneumonia]. / Serologicheskie raznovidnosti Klebsiella pneumoniae pri pnevmoniiakh.

118 K. pneumoniae cultures isolated from the pulmonary tissue samples, taken during autopsy from 33 patients who had died of pneumonia, and from the sputum of 19 living patients with chronic pneumonia, as well as 2 strains, K1 and K2, from the International Reference Klebsiella collection, formerly known as Friedländer's bacilli A and B, were studied. Besides K. pneumoniae K1 and K2, 10 other serovars of these bacteria, viz. K9, K11, K13, K16, K18, K19, K20, K62, K64, K82, were isolated, both as pure cultures and in association with other organisms, from the foci of inflammation in the pulmonary tissue in acute pneumonia. The K. pneumoniae serovars isolated from the sputum samples were essentially the same as those isolated from the autopsy material. The disease was found to have similar clinical and pathological manifestations irrespective of a K. pneumoniae serovar isolated in each particular case, which was indicative of the similar pattern of pulmonary tissue lesions characteristic of Friedländer's pneumonia. A new name for the nosological form of this disease is proposed: Klebsiella pneumonia instead of Friedländer's pneumonia. K. pneumoniae should be considered as pathogenic microorganisms, which was formerly known in respect of Friedländer's bacilli.

[Changes in the ischemic myocardium in experimental paired stimulation of the heart]. / Izmeneniia ishemizirovannogo miokarda pri parnoi stimuliatsii serdtsa v éksperimente.

In dog experiments the dynamics of electrophysiological and morphological indices of the ischemic myocardium in sinus tachycardia was compared with that in deceleration of cardiac contraction under the effect of paired stimulation. Intensification of the damaging effect of a 40-minute occlusion of the coronary artery complicated by destructive changes of the cardiomyocytes was revealed after deceleration of the rhythm; in reperfusion of the ischemic area the effect of electrogram restoration was diminished, which was attended with deeper focal damage to the heart muscle.

[Morphologic and histochemical changes in atrial and ventricular myocardium following adrenaline damage]. / Morfologicheskie i gistokhimicheskie izmeneniia miokarda zheludochkov i predserdii pri adrenalinovom povrezhdenii.

Changes in the myocardium developing after a single injection of toxic doses of adrenaline were studied. The course of adrenaline damage of the myocardium was shown to be wave-like. The most massive areas of degeneration and necrosis were observed on the 7th--14th day of the experiment. Ischemia developing because of the change of the microcirculation bed and impaired hemodynamics in the myocardium is a very important factor in the pathogenesis of adrenaline damage. The development of degeneration and necrotic areas in the auricles was found to occur later than in the ventricles which may be due to the ultrastructural features of atrial cardiomyocytes: the presence of electron dense granules, the destruction and decrease in the number of which coincide with the most marked signs of the heart muscle injury.

[Histoautoradiographic study of the heart in experimental myocardial ischemia]. / Gistoavtoradiograficheskoe issledovanie serdtsa pri éksperimental'noi ishemii miokarda.

Autoradiographic examinations of the heart muscle in experimental myocardial necroses using 3H-thymidine, revealed a high DNA synthesis in the connective tissue cells in the zone of necrosis in the acute period of infarction and its subsequent decrease. Deviations from this regularity were observed when relapses of necrosis developed. The activation of DNA synthesis occurred to a lesser extent in stromal cells of the periinfarction and remote zones of the heart. Muscle cells incorporated 3H-thymidine extremely rarely. When myocardial infarction was combined with aterosclerosis, relapses of necrosis occurred frequently, and morphological changes in many arteries and veins were accompanied by 3H-thymidine incorporation into the nuclei of the endothelium, smooth cells and adventitial cells. Inhibition of DNA synthesis in connective tissue cells of various heart zones was observed in cases of combined myocardial infarction and aterosclerosis and hypertension.

[DNA biosynthesis in the muscular and connective tissue cells of the heart in experimental myocardial infarct]. / Sintez DNK v myshechnykh i soedinitel'notkannykh kletkakh serdtsa pri eksperimental'nom infarkte.

In 60 rabbits with experimental myocardial infarction induces by ligation of the anterior branch of the left coronary artery, DNA synthesis was studied by means of H3-thymidine in muscular and connective tissue cells depending on the period of myocardial infarction. The development of myocardial infarction in the cardiac muscle was stated to be accompanied by activation of DNA synthesis in the connective tissue cells not only in the necrotic zone but in the adjacent, as well as in distant areas of the myocardium and in stromal cells of the auriculum. Indices of H3-thymidine labeled nuclei were of high value during the acute period of myocardial infarction and gradually decreased with the time elapsed since the operation. During the period of the myocardical infarction organization high activity in DNA synthesis was revealed in connective tissue elements of the epicardium and the subepicardial zone of the heart. Myocardial cells of the cardiac auriculi incorporated H3-thymidine but extremely seldom-single labels per thousands of nuclei. In the auriculi with application of prolonged sessions of the labeled precussor introduction, DNA synthesising nuclei were revealed, sometimes with paired nuclei of cardiomyocytes.

[Morphology and morphogenesis of recurrent experimental myocardial infarct]. / Morfologiia i morfogenez retsidiviruiushchego infarkta miokarda v éksperimente.

The results of morphological investigations of relapsing myocardial infarction in 148 rabbits are presented. In most cases the relapses occurred spontaneously at intervals of several to 45 days after reproduction of the primary myocardial infarction by high ligation of the anterior interventricle artery of the heart. It was found that relapses of myocardial infarction could develop in animals normal before the experiment (I series), against the background of cholesterol atherosclerosis (II series), renal hypertension (III series) and particularly frequently in combinations of cholesterol atherosclerosis and renal hypertension (IV series) as well as after repeated delayed ligation of the anterior interventricle artery (V series). The relapses occurred early in primary infarction and in the period of its scarring. The regularities of mutual location of foci of primary infarction and relapses, the frequency and number of their occurrences, location and size were investigated.

[Morphofunctional aspects of the experimental effect of hyperbaric oxygenation on the myocardium of the healthy organism]. / Morfofunktsional'nye aspekty vliianiia giperbaricheskoi oksigenatsii na miokard zdorovogo organizma v éksperimente.

Changes in the myocardium arter numerous treatments with hyperbaric oxygenation (HBO) (PO2 3 technical atmospheres, time of saturation--45 min) were studied by morphological, histochemical and enzymohistochemical methods in 50 rabbits. Three stages were found to occur in the development of these changes: 1) functional-morphological changes (1--3 seanses of HBO), 2) early morphological changes (4--5 seanses), 3) marked morphological lesions (6--8 seanses). The first three treatments produce reversible changes on the part of metabolic processes (a reduction in the content of glycogen and changes in the activity of oxidoreductases: succinate-, malate- and lactate dehydrogenase). Morphological signs of oxygen toxicity in the form of disorders in hemodynamics and microcirculation, and dystrophy of cardiomyocytes appear after 4 seanses of HBO and increase with further treatment. The experimental data permit to recommend short HBO courses (1--3 seanses) for therapeutic application.

[Patterns in changes in the dimensions of the nucleoli of human cardiac neurons during postnatal ontogenesis]. / Nekotorye zakonomernosti izmeneniia razmerov iadryshek neironov serdtsa cheloveka v postnatan'nom ontogeneze.

Morphometric studies of the neuronal nucleoli from the hearts of 157 healthy persons at different age, revealed certain regularities in their size changes, which demonstrate a strict quantitative organization--bilogical "quantumness"--of intranuclear synthesis processes and excretory processes into cytoplasm. Five more stable aliquot, as well as less stable, intermediate nucleolus volumes were demonstrated, their percentage content depending on the degree of differentiation, activity and cardiac neurons aging during postnatal ontogenesis.

[Morphological indicators of the peripheral blood leukocytes at different periods of development of myocardial infarct (experimental study)]. / Morfokunktsional'nye pokazateli leikotsitov perifericheskoi krovi v raznye sroki razvitiia infarkta miokarda (eksperimental'noe issledovanie).

The morpho-functional features of peripheral blood leukocytes were studied in 50 rabbits with experimental myocardial infarction at various intervals after ligation of the anterior interventricle artery. Changes in the leukocytes were compared with the morphological picture of myocardial infarction. In the acute period of experimental myocardial infarction not only quantitative changes were found to occur but also functional values of leukocytes changes: the content of glycogen and the activities of peroxidase and phagocytic activity of granulocytes were reduced, while the activity of alkaline phosphatase increased. Electron microscopic examinations of lymphocytes revealed ultrastructural changes in mitochondria. In the subacute period of the disease some of the values showed a trend for normalization. In the period of recuperation when the zone of infarction in rabbits is replaced by crude fiber connective tissue all the values under study in the peripheral blood became normal. The exceptions were the animals with extensive as well as complicated myocardial infarctions.