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1.
Adv Healthc Mater ; : e2301067, 2023 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-37479227

RESUMO

Organoids and cells in organ-on-chip platforms replicate higher-level anatomical, physiological, or pathological states of tissues and organs. These technologies are widely regarded by academia, the pharmacological industry and regulators as key biomedical developments. To map advances in this emerging field, a meta-analysis based on a quality-controlled text-mining algorithm is performed. The analysis covers titles, keywords, and abstracts of categorized academic publications in the literature and preprint databases published after 2010. The algorithm identifies and tracks 149 and 107 organs or organ substructures modeled as organoids and organ-on-chip, respectively, stem cell sources, as well as 130 diseases, and 16 groups of organisms other than human and mouse in which organoid/organ-on-chip technology is applied. The meta-analysis illustrates changing diversity and focus in organoid/organ-on-chip research and captures its geographical distribution. The downloadable dataset provided is a robust framework for researchers to interrogate with their own questions.

2.
PLoS One ; 10(4): e0121875, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25837972

RESUMO

Epichloë species (including the former genus Neotyphodium) are fungal symbionts of many agronomically important forage grasses, and provide their grass hosts with protection from a wide range of biotic and abiotic stresses. Epichloë species include many interspecific hybrids with allodiploid-like genomes, which may provide the potential for combined traits or recombination to generate new traits. Though circumstantial evidence suggests that such interspecific hybrids might have arisen from nuclear fusion events following vegetative hyphal fusion between different Epichloë strains, this hypothesis has not been addressed empirically. Here, we investigated vegetative hyphal fusion and subsequent nuclear behavior in Epichloë species. A majority of Epichloë strains, especially those having a sexual stage, underwent self vegetative hyphal fusion. Vegetative fusion also occurred between two hyphae from different Epichloë strains. Though Epichloë spp. are uninucleate fungi, hyphal fusion resulted in two nuclei stably sharing the same cytoplasm, which might ultimately lead to nuclear fusion. In addition, protoplast fusion experiments gave rise to uninucleate putative hybrids, which apparently had two markers, one from each parent within the same nucleus. These results are consistent with the notion that interspecific hybrids arise from vegetative hyphal fusion. However, we also discuss additional factors, such as post-hybridization selection, that may be important to explain the recognized prevalence of hybrids in Epichloë species.


Assuntos
Núcleo Celular/genética , Epichloe/genética , Proteínas Fúngicas/genética , Regulação Fúngica da Expressão Gênica , Hifas/genética , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Núcleo Celular/metabolismo , Núcleo Celular/ultraestrutura , Endófitos , Epichloe/classificação , Epichloe/metabolismo , Epichloe/ultraestrutura , Proteínas Fúngicas/metabolismo , Genes Reporter , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Hibridização Genética , Hifas/metabolismo , Hifas/ultraestrutura , Proteínas Luminescentes/genética , Proteínas Luminescentes/metabolismo , Fusão Nuclear , Filogenia , Plasmídeos/química , Plasmídeos/metabolismo , Poaceae/microbiologia , Poaceae/fisiologia , Protoplastos/metabolismo , Protoplastos/ultraestrutura , Esporos Fúngicos/genética , Esporos Fúngicos/metabolismo , Esporos Fúngicos/ultraestrutura , Simbiose/fisiologia
3.
Curr Opin Microbiol ; 20: 1-9, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24835421

RESUMO

Specific localization of appropriate sets of proteins and lipids is central to functions and integrity of organelles, which in turn underlie cellular activities of eukaryotes. Vesicle trafficking is a conserved mechanism of intracellular transport, which ensures such a specific localization to a subset of organelles. In this review article, we summarize recent advances in our understanding of how vesicle trafficking and related organelles support physiology and pathogenicity of filamentous fungi. Examples include a link between Golgi organization and polarity maintenance during hyphal tip growth, a new role of early endosomes in transport of translational machinery, involvement of endosomal/vacuolar compartments in secondary metabolite synthesis, and functions of vacuoles and autophagy in fungal development, nutrient recycling and allocation. Accumulating evidence showing the importance of unconventional secretion in fungal pathogenicity is also summarized.


Assuntos
Vesículas Citoplasmáticas/metabolismo , Retículo Endoplasmático/metabolismo , Fungos/fisiologia , Fungos/patogenicidade , Fungos/metabolismo
4.
Eukaryot Cell ; 11(12): 1463-71, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23042130

RESUMO

Hyphal anastomosis, or vegetative hyphal fusion, establishes the interconnection of individual hyphal strands into an integrated network of a fungal mycelium. In contrast to recent advances in the understanding of the molecular basis for hyphal anastomosis, knowledge of the physiological role of hyphal anastomosis in the natural habitats of filamentous fungi is still very limited. To investigate the role of hyphal anastomosis in fungal endophyte-plant interactions, we generated mutant strains lacking the Epichloë festucae soft (so) gene, an ortholog of the hyphal anastomosis gene so in the endophytic fungus E. festucae. The E. festucae Δso mutant strains grew similarly to the wild-type strain in culture but with reduced aerial hyphae and completely lacked hyphal anastomosis. The most striking phenotype of the E. festucae Δso mutant strain was that it failed to establish a mutualistic symbiosis with the tall fescue plant host (Lolium arundinaceum); instead, it killed the host plant within 2 months after the initial infection. Microscopic examination revealed that the death of the tall fescue plant host was associated with the distortion and disorganization of plant cells. This study suggests that hyphal anastomosis may have an important role in the establishment/maintenance of fungal endophyte-host plant mutualistic symbiosis.


Assuntos
Endófitos/genética , Deleção de Genes , Genes Fúngicos/genética , Hypocreales/genética , Lolium/microbiologia , Simbiose/genética , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Proteínas Fúngicas/toxicidade , Hifas/citologia , Hypocreales/citologia , Hypocreales/metabolismo , Hypocreales/patogenicidade , Lolium/citologia , Fenótipo
5.
Fungal Genet Biol ; 49(11): 896-902, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22939838

RESUMO

Mycelial interconnectedness achieved by hyphal fusion has been hypothesized to facilitate the distribution and sharing of nutrients between different parts of a mycelium, especially when nutrients are heterogeneously distributed in the environment. However, the link between environmental nutrient availability and hyphal fusion is little understood. Here, we report that amino acids and extracellular pH regulate conidial anastomosis tube (CAT) fusion during colony initiation in Neurospora crassa. Quantitative analyses revealed that low extracellular pH and certain amino acids, particularly tryptophan, inhibit CAT fusion. Conidial germination was also inhibited by tryptophan but this inhibition was mitigated by the presence of other amino acids. This provides evidence for tryptophan having a role as a signal molecule that regulates CAT fusion. Tryptophan acts intracellularly because two amino acid permease mutants (Δmtr and Δaap-20) exhibited resistance against tryptophan-mediated inhibition of CAT fusion. Tryptophan and low pH did not significantly affect vegetative hyphal fusion in mature colonies, indicating that the latter is regulated in a different manner to CAT fusion.


Assuntos
Neurospora crassa/metabolismo , Esporos Fúngicos/crescimento & desenvolvimento , Triptofano/metabolismo , Concentração de Íons de Hidrogênio , Hifas/genética , Hifas/crescimento & desenvolvimento , Hifas/metabolismo , Neurospora crassa/genética , Neurospora crassa/crescimento & desenvolvimento , Esporos Fúngicos/genética , Esporos Fúngicos/metabolismo
6.
PLoS One ; 7(2): e31175, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22319613

RESUMO

It has been hypothesized that horizontal gene/chromosome transfer and parasexual recombination following hyphal fusion between different strains may contribute to the emergence of wide genetic variability in plant pathogenic and other fungi. However, the significance of vegetative (heterokaryon) incompatibility responses, which commonly result in cell death, in preventing these processes is not known. In this study, we have assessed this issue following different types of hyphal fusion during colony initiation and in the mature colony. We used vegetatively compatible and incompatible strains of the common bean pathogen Colletotrichum lindemuthianum in which nuclei were labelled with either a green or red fluorescent protein in order to microscopically monitor the fates of nuclei and heterokaryotic cells following hyphal fusion. As opposed to fusion of hyphae in mature colonies that resulted in cell death within 3 h, fusions by conidial anastomosis tubes (CAT) between two incompatible strains during colony initiation did not induce the vegetative incompatibility response. Instead, fused conidia and germlings survived and formed heterokaryotic colonies that in turn produced uninucleate conidia that germinated to form colonies with phenotypic features different to those of either parental strain. Our results demonstrate that the vegetative incompatibility response is suppressed during colony initiation in C. lindemuthianum. Thus, CAT fusion may allow asexual fungi to increase their genetic diversity, and to acquire new pathogenic traits.


Assuntos
Fungos/genética , Variação Genética , Plantas/microbiologia , Núcleo Celular , Transferência Genética Horizontal , Hifas , Esporos Fúngicos
7.
Mol Microbiol ; 81(1): 40-55, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21564341

RESUMO

Although exocytosis in fungal cells takes place at hyphal tips, there also seems a line of circumstantial evidence suggesting the occurrence of exocytosis at other sites of cells, such as septa. To investigate whether exocytosis takes place at fungal septa, we monitored dynamics of EGFP-fused α-amylase (AmyB-EGFP), the representative secretory enzyme of the filamentous fungus Aspergillus oryzae. We found that AmyB-EGFP accumulates in Spitzenkörper at hyphal tips as well as septal periplasm between the plasma membrane and cell walls. The septal accumulation of AmyB-EGFP was a rapid process, and required microtubules but not F-actin. Thus, this process is independent of exocytosis at hyphal tips that requires both microtubules and F-actin. In addition, fluorescence recovery after photobleaching (FRAP) analysis of EGFP-fused AoSnc1 revealed that secretory vesicles constitutively fuse with the septal plasma membrane. These results demonstrated that exocytosis takes place at septa in addition to hyphal tips. Analysis of two plasma membrane transporters, AoUapC and AoGap1, revealed that they preferentially accumulate at septa and the lateral plasma membrane with no clear accumulation at apical Spitzenkörper, suggesting that non-tip directed exocytosis is important for delivery of these proteins.


Assuntos
Aspergillus oryzae/metabolismo , Exocitose , Hifas/metabolismo , Aspergillus oryzae/química , Genes Reporter , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Hifas/química , Proteínas de Membrana Transportadoras/metabolismo , Microtúbulos/metabolismo , Periplasma/química , Proteínas R-SNARE/genética , Proteínas R-SNARE/metabolismo , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , alfa-Amilases/genética , alfa-Amilases/metabolismo
8.
PLoS One ; 5(12): e15650, 2010 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-21187926

RESUMO

Filamentous fungi consist of continuum of multinucleate cells called hyphae, and proliferate by means of hyphal tip growth. Accordingly, research interest has been focusing on hyphal tip cells, but little is known about basal cells in colony interior that do not directly contribute to proliferation. Here, we show that autophagy mediates degradation of basal cell components in the filamentous fungus Aspergillus oryzae. In basal cells, enhanced green fluorescent protein (EGFP)-labeled peroxisomes, mitochondria, and even nuclei were taken up into vacuoles in an autophagy-dependent manner. During this process, crescents of autophagosome precursors matured into ring-like autophagosomes to encircle apparently whole nuclei. The ring-like autophagosomes then disappeared, followed by dispersal of the nuclear material throughout the vacuoles, suggesting the autophagy-mediated degradation of whole nuclei. We also demonstrated that colony growth in a nutrient-depleted medium was significantly inhibited in the absence of functional autophagy. This is a first report describing autophagy-mediated degradation of whole nuclei, as well as suggesting a novel strategy of filamentous fungi to degrade components of existing hyphae for use as nutrients to support mycelial growth in order to counteract starvation.


Assuntos
Aspergillus oryzae/metabolismo , Autofagia , Aspergillus oryzae/fisiologia , Núcleo Celular/metabolismo , Deleção de Genes , Proteínas de Fluorescência Verde/metabolismo , Lisossomos/metabolismo , Mitocôndrias/metabolismo , Fases de Leitura Aberta , Peroxissomos/metabolismo , Fagossomos/metabolismo , Reação em Cadeia da Polimerase/métodos , Vacúolos/metabolismo
9.
Eukaryot Cell ; 9(4): 547-57, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20139238

RESUMO

This study demonstrates the utility of Lifeact for the investigation of actin dynamics in Neurospora crassa and also represents the first report of simultaneous live-cell imaging of the actin and microtubule cytoskeletons in filamentous fungi. Lifeact is a 17-amino-acid peptide derived from the nonessential Saccharomyces cerevisiae actin-binding protein Abp140p. Fused to green fluorescent protein (GFP) or red fluorescent protein (TagRFP), Lifeact allowed live-cell imaging of actin patches, cables, and rings in N. crassa without interfering with cellular functions. Actin cables and patches localized to sites of active growth during the establishment and maintenance of cell polarity in germ tubes and conidial anastomosis tubes (CATs). Recurrent phases of formation and retrograde movement of complex arrays of actin cables were observed at growing tips of germ tubes and CATs. Two populations of actin patches exhibiting slow and fast movement were distinguished, and rapid (1.2 microm/s) saltatory transport of patches along cables was observed. Actin cables accumulated and subsequently condensed into actin rings associated with septum formation. F-actin organization was markedly different in the tip regions of mature hyphae and in germ tubes. Only mature hyphae displayed a subapical collar of actin patches and a concentration of F-actin within the core of the Spitzenkörper. Coexpression of Lifeact-TagRFP and beta-tubulin-GFP revealed distinct but interrelated localization patterns of F-actin and microtubules during the initiation and maintenance of tip growth.


Assuntos
Actinas/metabolismo , Corantes Fluorescentes/metabolismo , Proteínas Fúngicas/metabolismo , Neurospora crassa/metabolismo , Actinas/genética , Actinas/ultraestrutura , Compostos Bicíclicos Heterocíclicos com Pontes/farmacologia , Polaridade Celular , Citoesqueleto/efeitos dos fármacos , Citoesqueleto/metabolismo , Citoesqueleto/ultraestrutura , Proteínas Fúngicas/genética , Microtúbulos/efeitos dos fármacos , Microtúbulos/metabolismo , Microtúbulos/ultraestrutura , Neurospora crassa/crescimento & desenvolvimento , Neurospora crassa/ultraestrutura , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Tiazolidinas/farmacologia , Tubulina (Proteína)/genética , Tubulina (Proteína)/metabolismo
10.
Curr Opin Microbiol ; 12(6): 608-15, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19864177

RESUMO

The formation of interconnected hyphal networks is central to the organisation and functioning of the filamentous fungal colony. It is brought about by the fusion of specialised hyphae during colony initiation and mature colony development. These hyphae are normally genetically identical, and hence this process is termed hyphal self-fusion. The conidial anastomosis tube (CAT) functions in forming networks of conidial germlings during colony initiation. This hyphal type in Neurospora crassa is being used as a model for studies on hyphal self-signalling and self-fusion in filamentous fungi. Extraordinary new insights into the process of self-signalling that occurs during CAT self-fusion have recently been revealed by live-cell imaging of genetically engineered strains of N. crassa. A novel form of signalling involving the oscillatory recruitment of signal proteins to CAT tips that are communicating and growing towards each other has been observed. This 'ping-pong' mechanism operates over a very short time scale and comparisons with non-self-signalling during yeast cell mating indicate that this mechanism probably does not involve transcriptional regulation. It is proposed that this mechanism has evolved to increase the efficiency of fusion between genetically identical cells that are non-motile.


Assuntos
Fusão Celular , Regulação Fúngica da Expressão Gênica , Hifas/fisiologia , Neurospora crassa/fisiologia , Transdução de Sinais , Esporos Fúngicos/fisiologia , Modelos Biológicos
11.
Eukaryot Cell ; 8(1): 37-46, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19028995

RESUMO

Establishing the occurrence of endocytosis in filamentous fungi was elusive in the past mainly due to the lack of reliable indicators of endocytosis. Recently, however, it was shown that the fluorescent dye N-(3-triethylammoniumpropyl)-4-(p-diethyl-aminophenyl-hexatrienyl)pyridinium dibromide (FM4-64) and the plasma membrane protein AoUapC (Aspergillus oryzae UapC) fused to enhanced green fluorescent protein (EGFP) were internalized from the plasma membrane by endocytosis. Although the occurrence of endocytosis was clearly demonstrated, its physiological importance in filamentous fungi still remains largely unaddressed. We generated a strain in which A. oryzae end4 (Aoend4), the A. oryzae homolog of Saccharomyces cerevisiae END4/SLA2, was expressed from the Aoend4 locus under the control of a regulatable thiA promoter. The growth of this strain was severely impaired, and its hyphal morphology was altered in the Aoend4-repressed condition. Moreover, in the Aoend4-repressed condition, neither FM4-64 nor AoUapC-EGFP was internalized, indicating defective endocytosis. Furthermore, the localization of a secretory soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) was abnormal in the Aoend4-repressed condition. Aberrant accumulation of cell wall components was also observed by calcofluor white staining and transmission electron microscopy analysis, and several genes that encode cell wall-building enzymes were upregulated, indicating that the regulation of cell wall synthesis is abnormal in the Aoend4-repressed condition, whereas Aopil1 disruptants do not display the phenotype exhibited in the Aoend4-repressed condition. Our results strongly suggest that endocytosis is crucial for the hyphal tip growth in filamentous fungi.


Assuntos
Aspergillus oryzae/citologia , Membrana Celular/metabolismo , Endocitose , Aspergillus oryzae/genética , Aspergillus oryzae/metabolismo , Membrana Celular/genética , Polaridade Celular , Parede Celular/genética , Parede Celular/metabolismo , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Teste de Complementação Genética
13.
Biotechnol Lett ; 30(1): 7-14, 2008 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-17846708

RESUMO

Studies on protein production using filamentous fungi have mostly focused on improvement of the protein yields by genetic modifications such as overexpression. Recent genome sequencing in several filamentous fungal species now enables more systematic approaches based on reverse genetics and molecular biology of the secretion pathway. In this review, we summarize recent molecular-based advances in our understanding of vesicular trafficking in filamentous fungi, and discuss insights into their high secretion ability and application for protein production.


Assuntos
Proteínas Fúngicas/metabolismo , Fungos/metabolismo , Fungos/ultraestrutura , Perfilação da Expressão Gênica/métodos , Modelos Biológicos , Transdução de Sinais/fisiologia , Frações Subcelulares/metabolismo
14.
Biochem Biophys Res Commun ; 362(2): 474-9, 2007 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-17719006

RESUMO

Previously, we found that deletion of Aovps24, an ortholog of Saccharomyces cerevisiae VPS24, that encodes an ESCRT (endosomal sorting complex required for transport)-III component required for late endosomal function results in fragmented and aggregated vacuoles. Although defective late endosomal function is likely responsible for this phenotype, critical lack of our knowledge on late endosomes in filamentous fungi prevented us from further characterization. In this study, we identified late endosomes of Aspergillus oryzae, by expressing a series of fusion proteins of fluorescent proteins with orthologs of late endosomal proteins. Using these fusion proteins as markers, we observed late endosomes in the wild type strain and the Aovps24 disruptant and demonstrated that late endosomes are aberrantly aggregated in the Aovps24 disruptant. Moreover, we revealed that the aggregated late endosomes have features of vacuoles as well. As deletion of another ESCRT-III component-encoding gene, Aovps2, resulted in similar phenotypes to that in the Aovps24 disruptant, phenotypes of the Aovps24 disruptant are probably due to defective late endosomal function.


Assuntos
Aspergillus oryzae/metabolismo , Endossomos/metabolismo , Proteínas Fúngicas/metabolismo , Vacúolos/metabolismo , Proteínas de Transporte Vesicular/metabolismo , Aspergillus oryzae/genética , Proteínas Fúngicas/genética , Deleção de Genes , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Microscopia de Fluorescência , Transporte Proteico , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Proteínas de Transporte Vesicular/genética
15.
Fungal Genet Biol ; 44(12): 1310-23, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17590362

RESUMO

In spite of their great importance for both applied and basic biology, studies on vesicular trafficking in filamentous fungi have been so far very limited. Here, we identified 21 genes, which might be a total set, encoding putative SNARE proteins that are key factors for vesicular trafficking, taking advantage of available whole genome sequence in the filamentous fungus Aspergillus oryzae. The subsequent systematic analysis to determine the localization of putative SNAREs using EGFP-fused chimeras revealed that most putative SNAREs show similar subcellular distribution to their counterparts in the budding yeast. However, there existed some characteristic features of SNAREs in A. oryzae, such as SNARE localization at/near the septum and the presence of apparently non-redundant plasma membrane Qa-SNAREs. Overall, this analysis allowed us to provide an overview of vesicular trafficking and organelle distribution in A. oryzae.


Assuntos
Aspergillus oryzae/metabolismo , Proteínas Fúngicas/metabolismo , Proteínas SNARE/metabolismo , Aspergillus oryzae/genética , Membrana Celular/metabolismo , Proteínas Fúngicas/genética , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Microscopia de Fluorescência , Transporte Proteico/fisiologia , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Proteínas SNARE/genética
16.
Autophagy ; 2(3): 226-7, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16874107

RESUMO

Morphological analyses of vacuoles in filamentous fungi in the past decade have led to the remarkable finding that they are highly pleiomorphic organelles. Among them, tubular vacuoles have been implicated in nutrient transport between hyphal tips and the host plant surface in mycorrhizal fungi. However, a series of works suggested the presence of tubular vacuoles in other fungi that are not mycorrhizal, including Aspergillus oryzae, hinting at more general roles of the tubular vacuoles. Recently, we made two key observations by using the fusion protein of enhanced green fluorescent protein (EGFP) with a putative vacuolar t-SNARE in A. oryzae; tubular vacuoles formed more extensively in hyphae that were not in contact with nutrients, and vacuoles that were interconnected by tubules in the mature mycelial region displayed traces of microautophagy-mediated degradation of cytoplasm. The aim of this addendum is to discuss the possible involvement of vacuoles in degrading, transporting, and recycling nutrients from the mature mycelial region to hyphal tips, to support the continuous tip growth.


Assuntos
Alimentos , Fungos/fisiologia , Vacúolos/fisiologia , Aspergillus oryzae/crescimento & desenvolvimento , Aspergillus oryzae/fisiologia , Fungos/crescimento & desenvolvimento , Hifas/crescimento & desenvolvimento , Modelos Biológicos
17.
Eukaryot Cell ; 5(2): 411-21, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16467481

RESUMO

Vacuoles in filamentous fungi are highly pleomorphic and some of them, e.g., tubular vacuoles, are implicated in intra- and intercellular transport. In this report, we isolated Aovam3, the homologue of the Saccharomyces cerevisiae VAM3 gene that encodes the vacuolar syntaxin, from Aspergillus oryzae. In yeast complementation analyses, the expression of Aovam3 restored the phenotypes of both Deltavam3 and Deltapep12 mutants, suggesting that AoVam3p is likely the vacuolar and/or endosomal syntaxin in A. oryzae. FM4-64 [N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenyl-hexatrienyl)pyridinium dibromide] and CMAC (7-amino-4-chloromethylcoumarin) staining confirmed that the fusion protein of enhanced green fluorescent protein (EGFP) with AoVam3p (EGFP-AoVam3p) localized on the membrane of the pleomorphic vacuolar networks, including large spherical vacuoles, tubular vacuoles, and putative late endosomes/prevacuolar compartments. EGFP-AoVam3p-expressing strains allowed us to observe the dynamics of vacuoles with high resolutions, and moreover, led to the discovery of several new aspects of fungal vacuoles, which have not been discovered so far with conventional staining methods, during different developmental stages. In old hyphae, EGFP fluorescence was present in the entire lumen of large vacuoles, which occupied most of the cell, indicating that degradation of cytosolic materials had occurred in such hyphae via an autophagic process. In hyphae that were not in contact with nutrients, such as aerial hyphae and hyphae that grew on a glass surface, vacuoles were composed of small punctate structures and tubular elements that often formed reticulum-like networks. These observations imply the presence of so-far-unrecognized roles of vacuoles in the development of filamentous fungi.


Assuntos
Aspergillus oryzae/citologia , Membranas Intracelulares/metabolismo , Vacúolos/metabolismo , Aspergillus oryzae/crescimento & desenvolvimento , Aspergillus oryzae/metabolismo , Clonagem Molecular , Teste de Complementação Genética , Genótipo , Fenótipo , Transporte Proteico , Proteínas Qa-SNARE , Proteínas Recombinantes de Fusão
18.
Biochem Biophys Res Commun ; 340(3): 784-91, 2006 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-16380079

RESUMO

Endocytosis is an important process for cellular activities. However, in filamentous fungi, the existence of endocytosis has been so far elusive. In this study, we used AoUapC-EGFP, the fusion protein of a putative uric acid-xanthine permease with enhanced green fluorescent protein (EGFP) in Aspergillus oryzae, to examine whether the endocytic process occurs or not. Upon the addition of ammonium into the medium the fusion protein was internalized from the plasma membrane. The internalization of AoUapC-EGFP was completely blocked by sodium azide, cold, and cytochalasin A treatments, suggesting that the internalization possesses the general features of endocytosis. These results demonstrate the occurrence of endocytosis in filamentous fungi. Moreover, we discovered that the endosomal compartments appeared upon the induction of endocytosis and moved in a microtubule-dependent manner.


Assuntos
Aspergillus oryzae/metabolismo , Membrana Celular/metabolismo , Endocitose , Proteínas de Fluorescência Verde/metabolismo , Actinas/química , Temperatura Baixa , Citocalasinas/farmacologia , Endossomos , Proteínas Fúngicas/química , Proteínas de Membrana Transportadoras/química , Microscopia de Fluorescência , Microtúbulos/metabolismo , Plasmídeos/metabolismo , Compostos de Amônio Quaternário/química , Compostos de Amônio Quaternário/farmacologia , Proteínas Recombinantes de Fusão/química , Azida Sódica/farmacologia , Fatores de Tempo
19.
FEMS Microbiol Lett ; 244(1): 41-6, 2005 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-15727819

RESUMO

In filamentous fungi, the repertoire of promoters available for exogenous gene expression is limited. Here, we report the development and application of the thiamine-regulatable thiA promoter (PthiA) in Aspergillus oryzae as a tool for molecular biological studies. When PthiA was used to express the enhanced green fluorescent protein (EGFP) reporter, the fluorescence in the mycelia was either repressed or induced in the presence or absence of thiamine in the culture media, respectively. In addition, the expression level from the thiA promoter can be controlled by the concentration of external thiamine. Thiamine content in the media did not affect mycelial morphology, making the thiA promoter more useful compared with alcA and amyB promoters that depend on carbon source for regulation. Moreover, as the A. oryzae thiA promoter was also regulated by thiamine in A. nidulans, this promoter can be further applied as an inducible promoter in other Aspergilli.


Assuntos
Aspergillus oryzae/genética , Proteínas Fúngicas/genética , Genes Fúngicos , Regiões Promotoras Genéticas , Aspergillus oryzae/efeitos dos fármacos , Aspergillus oryzae/metabolismo , Sequência de Bases , DNA Complementar/genética , DNA Fúngico/genética , Regulação Fúngica da Expressão Gênica/efeitos dos fármacos , Proteínas de Fluorescência Verde/genética , Processamento Pós-Transcricional do RNA , Proteínas Recombinantes/genética , Tiamina/farmacologia
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