RESUMO
The analgesic activity of the methanolic extracts of the roots and leaves of Calliandra portoricensis was investigated in mice and rats using acetic acid-induced writhing and formalin tests. The extracts given orally at the doses of 200, 400, 600 mg/kg showed a dose-dependent activity in the tests used.
Assuntos
Analgésicos/farmacologia , Fabaceae , Dor/prevenção & controle , Fitoterapia , Extratos Vegetais/farmacologia , Ácido Acético , Administração Oral , Analgésicos/administração & dosagem , Analgésicos/uso terapêutico , Animais , Relação Dose-Resposta a Droga , Formaldeído , Masculino , Camundongos , Dor/induzido quimicamente , Medição da Dor/efeitos dos fármacos , Extratos Vegetais/administração & dosagem , Extratos Vegetais/uso terapêutico , Folhas de Planta , Raízes de Plantas , RatosRESUMO
Two phospholipase enzymes NN1 and NN2 were purified from the venom of Naja nigricolis nigricolis Reinhardt to apparent homogeneity. NN1 was purified by a two-step anion-exchange chromatography on DEAE-cellulose column while NN2 was purified by a combination of anion-exchange chromatography and gel filtration on Sephadex G-150. The enzyme NN1 moved homogenously on acrylamide gel as a monomer with a molecular weight of 65 kDa while NN2 was a dimer of 71 kDa. Both enzymes were clearly separated. Both enzymes hydrolyzed L-alpha-phosphatidyl choline with activities of 345.5 for NN1 and 727.8 micromol min(-1) x mg(-1) for NN2. The dimeric 71-kDa enzyme has a higher haemolytic and anticoagulant activity than the monomeric 65-kDa enzyme. It is apparent that the dimeric enzyme has a more pronounced activity than the monomer has, thus toxic activity may be related to the hydrolysis of phospholipids.
Assuntos
Venenos Elapídicos/enzimologia , Fosfolipases A/isolamento & purificação , Fosfolipases A/metabolismo , Animais , Anticoagulantes/farmacologia , Bovinos , Cromatografia em Gel , Cromatografia por Troca Iônica/métodos , Dimerização , Eletroforese em Gel de Poliacrilamida , Eritrócitos/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Isoenzimas/química , Isoenzimas/isolamento & purificação , Isoenzimas/metabolismo , Isoenzimas/farmacologia , Peso Molecular , Fosfolipases A/química , Fosfolipases A/farmacologiaRESUMO
This study examines the antimicrobial activity of the hexane, dichloromethane and methanol extracts of Lippia multiflora and carvacrol isolated from the hexane extract. The result shows the hexane extract to be the most active, while the methanol extract exhibited no antimicrobial activity. The isolated carvacrol from the hexane fraction showed tremendous antimicrobial activity. These results confirm the traditional uses of Lippia multiflora in the treatment of disease conditions due to microbes.
