RESUMO
Monoclonal antibody (MAb) PK-2 inhibited the in vitro growth of nine Mycoplasma mycoides subsp. mycoides small-colony strains. In contrast to the results with polyclonal antisera, growth inhibition by MAb PK-2 was specific for M. mycoides subsp. mycoides small-colony strains and constituted a reliable means of distinguishing them from other mycoplasmas.
Assuntos
Anticorpos Monoclonais , Doenças dos Bovinos/diagnóstico , Mycoplasma mycoides/imunologia , Mycoplasma mycoides/isolamento & purificação , Pleuropneumonia Contagiosa/diagnóstico , Animais , Anticorpos Antibacterianos/farmacologia , Anticorpos Monoclonais/farmacologia , Bovinos , Doenças dos Bovinos/imunologia , Reações Cruzadas , Mycoplasma mycoides/crescimento & desenvolvimento , Pleuropneumonia Contagiosa/imunologia , Especificidade da EspécieRESUMO
Monoclonal antibody (MAb) E8-18 reacted with four isolates of Mycoplasma capricolum subsp. capripneumoniae in Western blots identifying an epitope on a 24 kDa antigen (p24). MAb E8-18 did not react with 11 isolates belonging to four other Mycoplasma species or subspecies closely related to M. capricolum subsp. capripneumoniae. A combination of trypsin treatment of intact organisms and detergent-phase partitioning revealed p24 to be an integral M. capricolum subsp. capripneumoniae surface membrane protein.
