RESUMO
We evaluated 120 leukapheresis procedures (93 patients), in order to detect clinical factors that influence the efficiency of CD34+ collection using Cobe Spectra trade mark cell separators. Hematocrit was >27% and platelet count >30 000/microl in >95% of patients. Platelet transfusions were given if the postprocedure count was &<20 000/microl. Multiple regression analysis was used to analyze putative factors, and a predictive equation defined by stepwise regression modeling. The mean efficiency was 0.59 (s.d. 0.27). Sex (M>F; P=0.01), the volume processed (inversely; P=0.01) and CD34+ cell count (inversely; P=0.04) were associated with efficiency, whereas hematocrit, platelet or leukocyte count, catheter type and patient weight were not. The effect size for predictive factors was small (R(2)=0.21). Adverse events were limited to hypocalcemia. We conclude that female sex, volume processed and CD34+ cell count adversely influence the efficiency of CD34+ cell leukapheresis. However, the impact of volume and CD34+ cell count is small, and likely to be offset by the influence of these same factors on overall yield. Leukapheresis appears to be safe and efficient for autologous blood and marrow transplantation patients with hematocrit >27% and platelet count >30 000/microl.
Assuntos
Antígenos CD34/sangue , Células-Tronco Hematopoéticas/citologia , Leucaférese/métodos , Transplante de Células-Tronco/métodos , Antígenos CD4/sangue , Ciclofosfamida/uso terapêutico , Etoposídeo/uso terapêutico , Feminino , Fator Estimulador de Colônias de Granulócitos/uso terapêutico , Fator Estimulador de Colônias de Granulócitos e Macrófagos/uso terapêutico , Hematócrito , Mobilização de Células-Tronco Hematopoéticas/métodos , Humanos , Masculino , Contagem de Plaquetas , Proteínas Recombinantes , Reprodutibilidade dos Testes , Transplante de Células-Tronco/efeitos adversos , Resultado do TratamentoRESUMO
UNLABELLED: In obesity, serum growth hormone (GH) is usually low, confounding GH assessment of short obese children. We evaluated whether 24-h caloric restriction would permit better discrimination between normal GH secretion and GH deficiency (GHD) by elevating night GH levels. DESIGN AND PATIENTS: Serum was obtained every 20 minutes 2000-0800 h before and 2200-0400 h after 24 hours of caloric restriction (8% of usual calories) in 24 normal height children [14 normal (weight for height 10-90th percentile); 10 obese (weight for height > 95th percentile)] and in 31 short children (height shorter than -2.0 SD below mean for age). All samples from both nights per child were assayed for GH simultaneously to eliminate interassay variability. RESULTS: Mean GH increased significantly in all groups after caloric restriction (P < 0.01). Obese children had lower baseline mean GH and GH amplitude compared to normal (P < 0.01); GH increased into normal range after restriction. Basal GH studies in short children were not significantly below normal. Surprisingly, some with low stimulated GH increased their night GH into the normal range after caloric restriction. CONCLUSIONS: Caloric restriction for 24 h enhances night GH similarly in short and in normal children, and thus does not increase the diagnostic utility of night GH studies in non-obese short children. Caloric restriction reverses suppressed GH secretory state of obese children, perhaps by decreasing diet-dependent somatostatin inhibition of GH secretion.
Assuntos
Ritmo Circadiano/fisiologia , Ingestão de Energia , Hormônio do Crescimento/sangue , Adolescente , Determinação da Idade pelo Esqueleto , Estatura , Índice de Massa Corporal , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Obesidade/sangue , Fatores de TempoRESUMO
The effects of clonidine on Na+ pumping in motoneurons of the isolated frog spinal cord was investigated using sucrose gap recordings from ventral roots. Na+ pump activity, induced in motoneurons either by tetanizing the dorsal root or by rapidly exposing the cord to normal medium following 30 min in K(+)-free Ringer's solution (K(+)-activated hyperpolarizations), was increased by application of clonidine (100 microM). These actions of clonidine were blocked by the preferential alpha 2-adrenergic antagonist yohimbine, but not by alpha 1-adrenergic antagonist prazosin or the beta-blocker propranolol. Clonidine's effects on Na+ pumping appeared to be indirect (presumably via interneurons) because its effects on K(+)-activated hyperpolarizations were reduced by tetrodotoxin (TTX) or high concentrations of Mg2+. This indirect mechanism involved activation of non-NMDA excitatory amino acid receptors. Thus, in the presence of clonidine, CNQX, but not APH, limited the ability of clonidine to enhance K(+)-activated hyperpolarizations. The AMPA receptor may play a role in the process, K(+)-activated hyperpolarizations were augmented by the presence of AMPA; NMDA had no effect. The present results are consistent with the idea that activation of alpha 2-adrenoceptors produces the following: the release of excitatory amino acids from interneurons; the activation of non-NMDA receptors on motoneurons; increased Na+ influx and loading and increased Na+ pump activity.
Assuntos
2-Amino-5-fosfonovalerato/análogos & derivados , Clonidina/farmacologia , Neurônios Motores/efeitos dos fármacos , ATPase Trocadora de Sódio-Potássio/efeitos dos fármacos , Medula Espinal/efeitos dos fármacos , 6-Ciano-7-nitroquinoxalina-2,3-diona , Aminoácidos/metabolismo , Aminoácidos/farmacologia , Animais , N-Metilaspartato/farmacologia , Norepinefrina/farmacologia , Prazosina/farmacologia , Propranolol/farmacologia , Quinoxalinas/farmacologia , Rana pipiens , Medula Espinal/citologia , Tetrodotoxina/farmacologia , Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiônico/farmacologiaRESUMO
Sucrose gap recordings from the ventral roots of isolated, hemisected frog spinal cords were used to evaluate the effects of high concentrations of serotonin (5-HT) and alpha-methyl-5-HT (alpha-Me-5-HT) on the changes in motoneuron potential produced by dorsal root stimulation and by excitatory amino acids and agonists. Bath application of 5-HT in concentrations of 10 microM or greater produced a concentration-dependent motoneuron depolarization. Polysynaptic ventral root potentials evoked by dorsal root stimuli were reduced in both amplitude and area by 5-HT or alpha-Me-5-HT (both 100 microM). This may result from a reduction of the postsynaptic sensitivity of motoneurons to excitatory amino acid transmitters because 5-HT significantly depressed motoneuron depolarizations produced by addition of L-glutamate and L-aspartate to the superfusate. Similarly, 5-HT reduced depolarizations produced by the excitatory amino acid agonists N-methyl-D-aspartate (NMDA), quisqualate, alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA), and kainate. alpha-Me-5-HT reduced NMDA depolarizations. Tetrodotoxin (TTX) did not affect the ability of 5-HT to attenuate NMDA or kainate depolarizations, but did eliminate the 5-HT-induced attenuation of quisqualate and AMPA depolarizations. The glycine receptor site associated with the NMDA receptor did not appear to be affected by 5-HT because saturation of the site by excess glycine did not alter the 5-HT-induced depression of NMDA responses. The 5-HT1C/2 antagonist ketanserin and the 5-HT1A/2 antagonist spiperone significantly attenuated the 5-HT-induced depression of NMDA-depolarizations.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Aminoácidos/farmacologia , Neurônios Motores/efeitos dos fármacos , Receptores de Serotonina/fisiologia , Reflexo/efeitos dos fármacos , Medula Espinal/citologia , Animais , Técnicas In Vitro , Potenciais da Membrana/efeitos dos fármacos , Rana pipiens , Receptores de Aminoácido , Receptores de Superfície Celular/efeitos dos fármacos , Receptores de N-Metil-D-Aspartato/antagonistas & inibidores , Receptores de Serotonina/efeitos dos fármacos , Nervo Isquiático/citologia , Nervo Isquiático/efeitos dos fármacos , Serotonina/análogos & derivados , Serotonina/farmacologia , Medula Espinal/efeitos dos fármacos , Raízes Nervosas Espinhais/fisiologiaRESUMO
The effects of serotonin and excitatory amino acids on motoneurons were examined by sucrose gap recordings from the ventral root of the isolated, hemisected frog spinal cord superfused with magnesium-free, carbonate-buffered Ringer solution. Low concentrations of serotonin (0.1 microM) and the serotonin1A agonist 8-hydroxy-2-(n-dipropylamino)tetralin (8-OH-DPAT; 0.01 microM) significantly increased the duration and amplitude of the polysynaptic components of ventral root potentials produced by dorsal root stimulation. The facilitations of the ventral root potentials were blocked by the serotonin1A antagonist spiroxatrine, but were unaffected by the serotonin2 antagonist ketanserin or the serotonin3 antagonist 1 alpha H,3 alpha,5 alpha H-tropan-3-yl-3,-dichlorobenzoate (MDL 72222). The actions of 0.1 microM serotonin on motoneuron depolarizations evoked by the putative excitatory amino acid transmitters L-glutamate and L-aspartate were quite variable, but in the presence of ketanserin (20 microM), small consistent increases in amino acid-induced motoneuron depolarizations were observed. 8-OH-DPAT significantly enhanced motoneuron depolarizations elicited by the selective excitatory amino acid agonist N-methyl-D-aspartate in both normal and tetrodotoxin-containing Ringer solution. Quisqualate-induced motoneuron depolarizations were also facilitated by 8-OH-DPAT in normal Ringer solution, but these increases were eliminated by addition of either tetrodotoxin or the N-methyl-D-aspartate antagonist D(-)-2-amino-5-phosphonovalerate to the Ringer superfusate. Kainate-depolarizations were not altered by low concentrations of serotonin or 8-OH-DPAT. Prior exposure of the cord to spiperone, but not ketanserin or MDL 72222 blocked the enhancement of N-methyl-D-aspartate-induced motoneuron depolarizations by 8-OH-DPAT.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Vias Aferentes/fisiologia , Neurônios Motores/fisiologia , N-Metilaspartato/farmacologia , Serotonina/farmacologia , Medula Espinal/fisiologia , Tetra-Hidronaftalenos/farmacologia , 2-Amino-5-fosfonovalerato/farmacologia , 8-Hidroxi-2-(di-n-propilamino)tetralina , Animais , Estimulação Elétrica , Potenciais Evocados/efeitos dos fármacos , Glicina/farmacologia , Técnicas In Vitro , Ácido Caínico/farmacologia , Potenciais da Membrana/efeitos dos fármacos , Neurônios Motores/efeitos dos fármacos , Fibras Nervosas/efeitos dos fármacos , Fibras Nervosas/fisiologia , Ácido Quisquálico/farmacologia , Rana pipiens , Medula Espinal/efeitos dos fármacos , Raízes Nervosas Espinhais/efeitos dos fármacos , Raízes Nervosas Espinhais/fisiologia , Tetrodotoxina/farmacologia , Tropanos/farmacologiaRESUMO
Mice immunised by drinking water containing an Aro- mutant strain of Salmonella typhimurium produced intestinal IgA antibodies after a memory response which was demonstrated by measuring copro-antibodies. After oral challenge with a virulent strain of S. typhimurium, foster mouse pups placed with immunised mothers survived longer than control pups held with non-immunised mothers.
Assuntos
Imunização Passiva , Imunoglobulina A Secretora/biossíntese , Salmonelose Animal/imunologia , Salmonella typhimurium/imunologia , Animais , Animais Recém-Nascidos , Anticorpos Antibacterianos/biossíntese , Linhagem Celular , Epitélio/imunologia , Feminino , Mucosa Intestinal/imunologia , Camundongos , Camundongos Endogâmicos BALB C , GravidezRESUMO
Three lines of evidence are presented indicating that axons of the Aplysia neuroendocrine bag cells extend into the head-ring ganglia of the CNS. When the abdominal ganglion was bisected longitudinally, separating the two bag cell clusters, an afterdischarge induced in one cluster generated an afterdischarge in the other via activity through the head-ring ganglia to which each half abdominal ganglion was attached by connective nerves. This suggests that some axons of bag cells in each cluster communicate through the head-ring ganglia. Retrograde labelling of bag cells occurred when rhodamine-conjugated latex microspheres were injected into the cerebral or either pleural ganglion, a direct demonstration that bag cell axons extend into these ganglia. Finally, cell LP1 in the left pleural ganglion was inhibited during a bag cell afterdischarge, an action mimicked by application of alpha-bag cell peptide (alpha BCP). Since alpha BCP can act only close to its site of release due to susceptibility to peptidase activity, it is likely that LP1 inhibition is dependent on the local release of alpha BCP from bag cell neurites in the pleural ganglion. These results open new possibilities for how bag cell afterdischarges may be initiated and broaden the distribution of their effects.
Assuntos
Aplysia/fisiologia , Gânglios/citologia , Neurônios/fisiologia , Abdome , Animais , Comportamento Animal/fisiologia , Eletrofisiologia , Gânglios/fisiologia , Histocitoquímica , Látex , Microesferas , RodaminasRESUMO
The growth of Salmonella typhimurium under anaerobic conditions resulted in its greater ability to invade Henle 407 epithelial cells and in greater uptake by mouse peritoneal cells in vitro. Anaerobic growth also resulted in the repression of at least one major outer membrane protein.
Assuntos
Proteínas da Membrana Bacteriana Externa/biossíntese , Salmonella typhimurium/crescimento & desenvolvimento , Anaerobiose , Animais , Células Cultivadas , Epitélio/microbiologia , Gentamicinas/farmacologia , Camundongos , Camundongos Endogâmicos BALB C , Cavidade Peritoneal/citologia , Salmonella typhimurium/metabolismo , Salmonella typhimurium/patogenicidadeRESUMO
We have examined the effects of peptides on the neuroendocrine bag cells, the R2 neuron and the left upper quadrant (LUQ) neurons of the abdominal ganglion of Aplysia californica. Peptides include those extracted from the atrial gland, a reproductive organ; those released by an afterdischarge of the bag cells; and 2 synthetic peptides: the amidated 9-amino acid C-terminal portion of atrial gland peptides A/B/ERH (B26-34), and the 8-amino acid alpha-bag cell peptide (alpha-BCP1-8). Peptides were applied by superfusion, arterial perfusion, pressure ejection from micropipettes, or by inducing a bag cell afterdischarge. Both alpha-BCP1-8 and B26-34 are able to produce a bag cell afterdischarge when applied to the abdominal ganglion but are not as effectively able to trigger the bag cells when applied selectively to the ganglia of the head ring. Peptides released by the bag cells inhibit R2 and LUQ neurons; whereas atrial gland extract mildly excites LUQ neurons and powerfully excites R2. The inhibitory effect of the LUQ cells and R2 following an afterdischarge of the bag cells is mimicked by alpha-BCP1-8. The excitatory effect of the atrial gland extract cannot be duplicated with B26-34. Rather, instead of having an excitatory effect on R2 and LUQ cells, B26-34 seems to mimick alpha-BCP1-8 and inhibit these neurons. Both peptides produce a membrane conductance increase in R2 and LUQ cells.