[Study of the morphofunctional properties of cultured thyrocytes of newborn piglets with the purpose of determining the feasibility of their use for compensation of thyroid gland hypofunction]. / Izuchenie morfofunktsional'nykh svoistv kul'tiviruemykh tirotsitov novorozhdennykh porosiat s tsel'iu opredelniia vozmozhnosti ikh primeneniia dlia kompensatsii gipofunktsii shchitovidnoi zelezy.

The method for cultivation has been developed and morphofunctional characteristics of the newborn piglet thyrocytes studied in order to elucidate the possibility of using these cells for the compensation of the thyroid gland hypofunctioning. The results evidence viability of the cultivated thyrocytes and intactness of their thyroxine-secreting activity within 14 days. Addition of thyrotropin to the medium resulted in the accrement in the population of the active hormone-producing and follicle-producing cells, in reduction of the number of degenerating and pyknotic forms, in stimulation of thyroxine secretion, incorporation of 3H-leucin in thyrocyte protein, and in absorption and organification of 131I by these cells, this being the specific function of this gland, providing the adequate synthesis of thyroid hormones. The resultant thyrocyte culture may be used in clinical transplantology for the treatment of hypofunctional states of the thyroid.

[The effect of biologically active substances from the spleen on the content of somatotropic hormone in the rat hypophysis]. / Vliianie biologicheski aktivnykh veshchestv selezenki na soderzhanie somatotropnogo gormona v gipofize krys.

The influence of splenin, a "splenic protein-free extract", and splenectomy on the somatotropic hormone content in the rat pituitary were investigated. It was established that the concentration of this pituitary hormone was only decreased after "protein-free extract" administration. It was suggested that the detected effect was due to peptides incorporated in the preparation composition.

[Somatotropin-synthesizing activity of the in vitro cultured adenohypophyses of intact, hypo- and hyperthyroid rats]. / Somatotropinsinteziruiushchaia aktivnost' kul'tiviruemykh vne organizma adenogipofizov intaktnykh, gipo- i gipertireoidnykh krys.

Somatotropin-synthetizing activity of the adenohypophyses cultivated in vitro in eu-, hupo- and hyperthyroid rats was investigated. The most pronounced persistence of the activity (89%) within 12 days is seen in the adenohypophyses of hypothyroid rats and less evident (41%) in the adenohypophyses of hyperthyroid animals in comparison with that of euthyroid rats (66%). The activity rise in the adenohypophyses of euthyroid hypothyroid rats is observed by the 6th day of cultivation, being more pronounced in the adenohypophyses of hypo- than euthyroid animals. The adenohypophyses cultivated in vitro in hypo- and hyperthyroid rats may serve as models for studying the adenohypophyseal somatotropic function disorders in hypo- and hyperthyroses.

[Somatotropic function of the adenohypophysis in hyperthyroidism]. / Somatotropnaia funktsiia adenogipofiza pri gipertireoze.

Changes in the adenohypophyseal somatotropin function and the site of their origin were studied in experimental rats with hyperthyrosis. It was determined that the adenohypophyseal somatotropin content and biosynthesis were sharply reduced. After repeated somatotropin injections these indices remained unchanged. In vitro the somatotropin-releasing activity of the hypothalamic extracts off hyperthyroid rats is enhanced, whereas somatotropin release into the medium, obtained from the adenohypophyses of hyperthyroid animals, is lowered under the extract effect. It is concluded that a decrease in the adenohypophyseal somatotropin function is caused by loss of somatotropic cell sensitivity to the action of the hypothalamic regulation factors.

[Determination of somatotropin and prolactin by dodecyl sulfate polyacrylamide gel electrophoresis]. / Opredelenie somatotropina i prolaktina dodetsilsul'fat-élektroforezom v poliakrilamidnom gele.

Definite separation of somatotropin and prolactin using sodium dodecylsulphate polyacrylamide gel electrophoresis takes place in continuous systems with pH 7.0-7.2 containing acetate or chloride monovalet anions; substitution of the ions by polyvalent phosphate or borate anions interfered with the separation. The hormones precipitated by trichloracetic acid may be separated and quantitatively determined after dissolving in the weak alkaline solutions. The hormones are distinctly separated from serum proteins and may be quantitatively determined in media containing up to 15% of serum.