In-vivo anti-epileptic study of newly synthesized pregabalin derivatives based on docking studies.

OBJECTIVE: The goal of the present study is to examine pretreatment with Schiff bases and derivatives of pregabalin along with their metal (Zn and Cu) complexes on the severity of epilepsy, latency time, duration of convulsions, seizure score and survival rate in mice. METHODS: To achieve the goal, a molecular docking study of analogues was carried out on a specific molecular target, such as the alpha-2δ receptor (PDB ID: 6ND9); which revealed the significant binding affinity of the analogs to their respective target. Based on the docking information, all pregabalin derivatives were synthesized and in-vivo antiepileptic effect was confirmed by applying the PTZ model that prioritized the most crucial significant points responsible for biological activity. RESULTS: The test compounds markedly increased the latency of the first seizure and reduced the frequency of seizures throughout the body and frequent spinning and jumps. Additionally, treatment with pregabalin derivatives in mice that received PTZ significantly reduced the duration of seizures and seizure score. However, it increased the survival rate of the mice. DISCUSSION: Since the newly synthesized compounds were more active as compared to the parent drug in some respects; therefore, the expansion of the project can be planned to explore clinical side of the drugs in the future.

Docking studies of Schiff bases and derivatives of Pregabalin along with their Zn and Cu metal complexesPretreatment with Schiff bases and derivatives of pregabalin along with their metal (Zn and Cu)PTZ Model of epilepsyObservation of different parameters including; the severity of epilepsy, latency time, duration of convulsions, seizure score and survival rate in miceNewly synthesized compounds were more active as compared to the parent drug.

Three dimensional study for entropy optimization in nanofluid flow through a compliant curved duct: A drug delivery and therapy application.

This research explores the three-dimensional characteristics of nanofluid dynamics within curved ducts, in contrast to earlier studies that mainly focus on two-dimensional flow. By using this ground-breaking method, we can capture a more accurate depiction of fluid behavior that complies with the intricate duct design. In this study, we investigate the three dimensional flow and entropic analysis of peristaltic nanofluid flows in a flexible curved duct, comparing the effects of silver and copper nanoparticles. To obtain accurate results, we assume physical constraints such as long wavelength and low Reynolds number and used a perturbation technique through NDSolve commands for finding exact solutions of the obtained differential equations. A comprehensive error analysis is provided through residual error table and figures to estimate a suitable range of the physical factors. Our findings indicate that the velocity of the nanofluid is directly proportional to the elasticity of the walls, while the mass per unit volume inversely affects velocity. We show that reducing the aspect ratio of the duct rectangular section can decrease entropy generation by raising magnitudes of damping force exerted by to the flexible walls of the enclosure. Additionally, using a larger height of the channel than the breadth can reduce stream boluses. The practical implications of this study extend beyond turbines and endoscopy to biomedical processes such as drug delivery and microfluidic systems.