RESUMO
The aim of this study was to investigate the protective effect of L-carnitine (L-CAR) in tamoxifen (TAM)-induced toxicity and antitumor activity. Adult female rats were randomly divided into four groups. Group I was served as control, groups II and III were treated with TAM (10 mg/kg, periorally) and L-CAR (300 mg/kg, intraperitoneally), respectively, while group IV was treated with both compounds. The treatment continued daily for 28 days. Administration of TAM resulted in significant increase in serum lipid profiles, liver enzymes, and bilirubin level. TAM produced a significant increase in lipid peroxides (LPO) level and nonsignificant change in nitrogen oxide (NO(x)) level accompanied with significant decrease in superoxide dismutase (SOD) activity of hepatic and uterus tissues and significant decrease in glutathione (GSH) content of uterus tissue. Administration of L-CAR for 1 h prior to TAM treatment decreased serum lipids and liver enzymes significantly and significantly increased SOD activity in liver and uterus tissues compared with TAM-treated group. Furthermore, it restored LPO and GSH levels and increased NO(x) level in uterus tissue. DNA fragmentation and the apoptotic marker, caspase-3, were not detected in the liver of all treated groups. Histopathologically, alterations in the liver and uterus structures after TAM treatment, which was attenuated after L-CAR administration. The antitumor effect and survival of the combined treatment of Ehrlich ascites carcinoma (EAC)-bearing mice was less than each one alone. L-CAR interestingly increased survival rate of EAC-bearing mice more than TAM-treated group. In conclusion, L-CAR has beneficial effects regarding TAM toxicity; however, it interferes with its antitumor effect.
Assuntos
Carcinoma de Ehrlich/tratamento farmacológico , Carnitina/farmacologia , Tamoxifeno/farmacologia , Tamoxifeno/toxicidade , Animais , Fragmentação do DNA , Interações Medicamentosas , Feminino , Peróxidos Lipídicos/metabolismo , Fígado/efeitos dos fármacos , Camundongos , Neoplasias Experimentais/tratamento farmacológico , Óxidos de Nitrogênio/metabolismo , Estresse Oxidativo , Distribuição Aleatória , Ratos , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismo , Útero/efeitos dos fármacosRESUMO
The aim of this study was to examine the antitumour activity of resveratrol in human colorectal cancer cell lines (HCT116 and Caco2) and to explore its mechanism of action assuming that it is by calorie-restriction effect. Resveratrol inhibited the proliferation of colon cancer cells with half maximal inhibitory concentration (IC(50)) equal to 50 and 130 µM for HCT116 and Caco2, respectively. Caco2 cells appeared with significant time-dependent increase in the glycolytic pathway, a behaviour that was absent in HCT116 cells. Resveratrol (100 µM) significantly decreased the glycolytic enzymes (pyruvate kinase and lactate dehydrogenase) in Caco2 cells, while an increase in citrate synthase activity and a decrease in glucose consumption were observed in both cell lines. Moreover, resveratrol downregulated the expressions of leptin and c-Myc, and decreased the content of vascular endothelial growth factor. The apoptotic markers, caspases 3 and 8, were activated and the Bax/BCl2 ratio was increased. The study suggested a promising anticancer activity of resveratrol, calorie-restriction pathway may be one of the driving forces for this activity.
Assuntos
Antineoplásicos/farmacologia , Estilbenos/farmacologia , Apoptose/efeitos dos fármacos , Células CACO-2 , Restrição Calórica , Proliferação de Células/efeitos dos fármacos , Citrato (si)-Sintase/metabolismo , Glucose/metabolismo , Células HCT116 , Humanos , L-Lactato Desidrogenase/metabolismo , Leptina/metabolismo , Neovascularização Fisiológica/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-myc/metabolismo , Piruvato Quinase/metabolismo , Resveratrol , Fator A de Crescimento do Endotélio Vascular/metabolismoRESUMO
Pharmacological and cytogenetic evaluations of the protective effects of polyethoxylated castor oil cremophor-EL (cremophor) against hepato, renal and bone marrow toxicity induced by gamma irradiation in normal rats were carried out. A single dose of irradiation (6 Gy) caused hepatic and renal damage manifested biochemically as an elevation in levels of serum alanine and aspartate aminotransferase as well as an increase in blood urea. Cremophor administration at a dose level of 50 microl kg-1 intravenously 1 day before exposure to irradiation (6 Gy) protected the liver and kidney as indicated by the recovery of levels of hepatic aminotransferase, urea and lipid profiles to normal values. Gamma irradiation of male rats caused a decrease in reduced glutathione and an increase in the oxidized form in rat-liver homogenate. A highly significant increase in the incidence of micronucleated normochromatic erythrocytes and micronucleated polychromatic erythrocytes was observed after irradiation exposure. The induced genotoxicity in the bone marrow cells was corrected by pretreatment with cremophor. The findings of this study suggest that cremophor pretreatment can potentially be used clinically to prevent irradiation-induced hepato, renal and bone marrow toxicity without interference with its cytotoxic activity.
Assuntos
Raios gama , Glicerol/farmacologia , Hiperlipidemias/sangue , Rim/efeitos dos fármacos , Fígado/efeitos dos fármacos , Tensoativos/farmacologia , Alanina Transaminase/sangue , Alanina Transaminase/efeitos dos fármacos , Alanina Transaminase/efeitos da radiação , Animais , Aspartato Aminotransferases/sangue , Aspartato Aminotransferases/efeitos dos fármacos , Aspartato Aminotransferases/efeitos da radiação , Células da Medula Óssea/efeitos dos fármacos , Células da Medula Óssea/efeitos da radiação , Colesterol/sangue , Colesterol/efeitos da radiação , Creatinina/sangue , Creatinina/efeitos da radiação , Raios gama/efeitos adversos , Glutationa/efeitos dos fármacos , Glutationa/metabolismo , Glutationa/efeitos da radiação , Glicerol/análogos & derivados , Glicerol/uso terapêutico , Hiperlipidemias/tratamento farmacológico , Rim/metabolismo , Rim/efeitos da radiação , Fígado/metabolismo , Fígado/efeitos da radiação , Masculino , Camundongos , Micronúcleos com Defeito Cromossômico/efeitos dos fármacos , Micronúcleos com Defeito Cromossômico/metabolismo , Micronúcleos com Defeito Cromossômico/efeitos da radiação , Ratos , Ratos Wistar , Tensoativos/uso terapêutico , Triglicerídeos/sangue , Triglicerídeos/efeitos da radiação , Células Tumorais Cultivadas , Ureia/sangue , Ureia/efeitos da radiaçãoRESUMO
Propionyl-L-carnitine (PLC), a natural short-chain derivative of L-carnitine, has been tested in this study as a potential protective agent against adriamycin (ADR)-induced cardiotoxicity in isolated rat heart myocytes and mitochondria. In cardiac myocytes, ADR (0.5 mM) caused a significant (70%) inhibition of palmitate oxidation, whereas, PLC (5 mM) induced a significant (49%) stimulation. Addition of PLC to ADR-incubated myocytes induced 79% reversal of ADR-induced inhibition of palmitate oxidation. In isolated rat heart mitochondria, ADR produced concentration-dependent inhibition of both palmitoyl-CoA and palmitoyl-carnitine oxidation, while PLC caused a more than 2.5-fold increase in both substrates. Preincubation of mitochondria with 5 mM PLC caused complete reversal of ADR-induced inhibition in the oxidation of both substrates. Also ADR induced concentration-dependent inhibition of CPT I which is parallel to the inhibition of its substrate palmitoyl-CoA. In rat heart slices, ADR induced a significant (65%) decrease in adenosine triphosphate (ATP) and this effect is reduced to 17% only by PLC. Results of this study revealed that ADR induced its cardiotoxicity by inhibition of CPT I and beta-oxidation of long-chain fatty acids with the consequent depletion of ATP in cardiac tissues, and that PLC can be used as a protective agent against ADR-induced cardiotoxicity.
Assuntos
Antibióticos Antineoplásicos/antagonistas & inibidores , Antibióticos Antineoplásicos/toxicidade , Cardiotônicos/farmacologia , Carnitina/análogos & derivados , Doxorrubicina/antagonistas & inibidores , Doxorrubicina/toxicidade , Ácidos Graxos/metabolismo , Mitocôndrias Cardíacas/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Cardiotônicos/metabolismo , Carnitina/metabolismo , Carnitina/farmacologia , Carnitina O-Palmitoiltransferase/metabolismo , Técnicas In Vitro , Masculino , Mitocôndrias Cardíacas/efeitos dos fármacos , Miocárdio/citologia , Miocárdio/metabolismo , Oxirredução , Palmitatos/metabolismo , Palmitoil Coenzima A/metabolismo , Proteínas/metabolismo , Ratos , Ratos Sprague-DawleyRESUMO
Biopharmacological evaluations of the protective effects of L-carnitine (a naturally occurring quaternary ammonium compound) against doxorubicin-induced metabolic damage were carried out in isolated cardiac myocytes and in isolated rat heart mitochondria. Perfusion of the heart with DOX (0.5 mM) caused a significant 70% inhibition of palmitate oxidation in cardiac myocytes, while L-carnitine (5 mM) perfusion caused stimulation which accounted for 37%. Perfusion of the heart with L-carnitine after 10-min perfusion with DOX (0.5 mM) caused 88% reversal of DOX-induced inhibition of palmitate oxidation in cardiac cells. In rat heart mitochondria, DOX has no effect on either palmitate oxidation or acyl-CoA synthetase activity, whereas Enoximone (c-AMP-dependent phosphodiesterase inhibitor), caused a significant inhibition of palmitate oxidation and acyl-CoA activity (40 and 27%, respectively). The oxidation of palmitoyl-CoA, an index of carnitine palmitoyltransferse reaction was significantly inhibited by DOX as a function of DOX concentration. Preincubation of mitochondria with L-carnitine caused reversal of DOX-induced inhibition of palmitoyl-CoA oxidation depending on the concentration of L-carnitine. Moreover, L-carnitine treatment did not interfere with the cytotoxic effect of doxorubicin against the growth of solid Ehrlich carcinoma. The findings of this study may suggest that inhibition of fatty acid oxidation in the heart is at least a part of doxorubicin cardiotoxicity and that L-carnitine can be used to prevent the doxorubcin-induced cardiac metabolic damage without interfering with its antitumour activities.
Assuntos
Antineoplásicos/toxicidade , Cardiomiopatias/tratamento farmacológico , Cardiomiopatias/metabolismo , Carnitina/farmacologia , Doxorrubicina/toxicidade , Animais , Antineoplásicos/antagonistas & inibidores , Antineoplásicos/farmacologia , Carcinoma de Ehrlich/tratamento farmacológico , Carcinoma de Ehrlich/patologia , Cardiomiopatias/induzido quimicamente , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Relação Dose-Resposta a Droga , Doxorrubicina/antagonistas & inibidores , Doxorrubicina/farmacologia , Feminino , Técnicas In Vitro , Masculino , Camundongos , Oxirredução , Ácido Palmítico/metabolismo , Ratos , Ratos Sprague-DawleyRESUMO
Chloroacetonitrile (CAN), a drinking water disinfectant by-product, has mutagenic and carcinogenic properties. CAN is known to deplete glutathione (GSH), and previous studies reported an enhanced molecular interaction of CAN after GSH depletion in the uterine and fetal tissues of mice. The present report may help to understand the potential mechanisms involved in such molecular interactions by examining the disposition, transplacental uptake and covalent interaction of the chemical in normal and GSH depleted pregnant mice (at 13th day of gestation). Both normal and GSH depleted (by administration of Diethylmaleate (DEM), 0.6 mL/kg, i.p.) pregnant mice were given an equitoxic i.v. dose of 2-[14C]-CAN(333 microCi/kg equivalent to 77 mg/kg). Animals were processed for whole-body autoradiography (WBA) at 1, 8 and 24 hr after treatment. Tissue distribution of radioactivity in the autoradiographs was quantitated using computer aided image analysis. With few exceptions, a rapid high uptake (at 1 hr) of radioactivity was observed in all major maternal (liver, lung, urinary bladder, gastrointestinal mucosa, cerebellum, uterine luminal fluid) and fetal (liver, brain) organs of both normal and GSH depleted mice. This pattern of distribution was observed, with lesser intensity, at 8 hr following treatment. At a later time period (24 hr), there was a significant higher retention and covalent interaction of radioactivity in GSH depleted mouse tissues especially in the liver as compared to normal mouse. This study suggests that 2-[14C]-CAN and/or its metabolites are capable of crossing the placental barrier. The observed higher uptake and retention of the radioactivity in the maternal liver, kidney, cerebellum, nasal turbinates and fetal liver may pose toxicity of the chemical to these organs. The increased covalent interaction of radioactivty in GSH depleted mice liver may indicate the potential utilization of GSH pathway by this organ in the detoxication of CAN derived metabolites and thus exerting hepatotoxicity.
Assuntos
Acetonitrilas/farmacocinética , Glutationa/farmacologia , Acetonitrilas/toxicidade , Animais , Autorradiografia , Radioisótopos de Carbono , Feminino , Fígado/efeitos dos fármacos , Troca Materno-Fetal , Camundongos , Gravidez , Distribuição Tecidual , Abastecimento de Água/normasRESUMO
Worldwide usage of different colouring agents in the food industry prompted us to study their toxicity. The potential adverse effects of erythrosine (ER, FD & C Red No. 3) on the spermatogenesis process were investigated in adult mice. Testicular lactic dehydrogenase isoenzyme activity (LDH-X), a pachytene spermatocyte marker of testicular toxicity, was significantly decreased to 71.8% and 68.6% of the control value after daily p.o. administration of ER (21 days) in doses of 68 and 136 mg kg-1 respectively. At the same time, the normal average epididymal sperm count as well as the percentage of motile sperms were significantly inhibited by about 50% and 57% respectively. Moreover, ER was shown to disrupt the normal morphology of the sperm head. Thus, after 5 daily p.o administrations of ER in doses of 680 and 1360 mg kg-1 (equivalent to 10 and 20% of its LD50) it increased the incidence of sperms with abnormal head by about 57% and 65% respectively. The induced increase in sperm abnormalities could enhance the spermatogenic dysfunction and germ cell mutagenicity. These findings indicate that ER in the used doses has a potential toxic effect on spermatogenesis in mice and in turn, it may affect its testicular function and reproductive performance.
Assuntos
Corantes/toxicidade , Eritrosina/toxicidade , Infertilidade Masculina/induzido quimicamente , Animais , Isoenzimas , L-Lactato Desidrogenase/metabolismo , Masculino , Camundongos , Mutação , Contagem de Espermatozoides/efeitos dos fármacos , Cabeça do Espermatozoide/efeitos dos fármacos , Cabeça do Espermatozoide/ultraestrutura , Motilidade dos Espermatozoides/efeitos dos fármacos , Espermatogênese/efeitos dos fármacos , Testículo/citologia , Testículo/efeitos dos fármacos , Testículo/enzimologiaRESUMO
Binding of haloacetonitriles or their reactive metabolites to macromolecules of fetal tissue may be responsible for reproductive toxicity. To investigate the role of glutathione (GSH) in the metabolism and reproductive toxicity of haloacetonitriles, irreversible interaction of chloroacetonitrile (CAN) with maternal uterine and fetal DNA was assessed in a time course study among normal and among glutathione-depleted mice treated with [2-14C]-CAN. GSH was depleted in maternal and fetal tissues by treating of animals with diethylmaleate (DEM) 1 h before [2-14C]-CAN administration. Maternal urinary excretion of thiocyanate was 5 times higher in glutathione-depleted mice than in controls. At 8 and 24 h following [2-14C]-CAN administration, total radioactivity uptake in maternal uterine tissue, amniotic fluid, and fetal tissue was higher in glutathione-depleted mice than in control. Also the interaction of CAN or its reactive metabolites with maternal uterine DNA was enhanced following glutathione depletion. At 24 h after treatment, the covalent binding to DNA in fetal tissue was significantly increased in glutathione depleted mice (205% of control). The magnitude of interaction of CAN in fetal DNA was about 4 times higher than that in uterine DNA. The time course study in either maternal uterine or fetal DNA revealed elevated and persistent levels of covalent binding of [ C]-CAN to DNA at 72 h after treatment. Enhancement of the molecular interaction of CAN in maternal and fetal DNA following GSH depletion indicates an important role for GSH in CAN metabolism.
Assuntos
Acetonitrilas/toxicidade , DNA/efeitos dos fármacos , Feto/efeitos dos fármacos , Feto/metabolismo , Glutationa/metabolismo , Complicações na Gravidez/induzido quimicamente , Complicações na Gravidez/metabolismo , Acetonitrilas/metabolismo , Acetonitrilas/farmacocinética , Animais , Biotransformação , Radioisótopos de Carbono , DNA/metabolismo , DNA/urina , Feminino , Glutationa/deficiência , Inativação Metabólica , Maleatos/farmacologia , Camundongos , Gravidez , Complicações na Gravidez/urinaRESUMO
Acrylonitrile (VCN)-induced lung toxicity was studied following a single oral dose (46.5 mg/kg). The mechanism of toxic injury was investigated by assessing the covalent interaction of [2,3-14C]VCN with pulmonary DNA. The effect of the same dose on replicative DNA synthesis and repair in the lungs of treated rats was also investigated. Histologic examination revealed that lungs of VCN-treated animals showed moderate to marked hyperplasia of the Clara cells lining the bronchioles. [14C]Lung tissue uptake was extremely fast, having a maximum at 0.5 h after treatment (150 DPM tissue). Radioactivity in lung tissue declined gradually as a function of time, but was still detected at 72 h after treatment (59 DPM/mg tissue). Covalent binding of [14C]VCN-derived radioactivity to pulmonary DNA was time-dependent, reaching a maximum at 12 h following treatment (61 DPM/mg DNA) and was still detected at 72 h (27 DPM/mg DNA) indicating the incomplete removal of radioactivity covalently bound to DNA. Replicative DNA synthesis in lung tissue was significantly decreased at all time points studied (59, 55 and 72% of control at 0.5, 6 and 24 h, respectively). The DNA repair in the lung was increased by 2-fold at 0.5 h and 1.6-fold at 6 h following VCN oral treatment. The histologic and biochemical results presented in this study provide evidence for the acute genetic toxicity of VCN (and/or its metabolites) in lung tissue following a single oral dose of VCN.