Clinical effect of Chinese and western medicine in the treatment of gastric emptying disorder after gastric cancer surgery / 中国基层医药

Objective To observe the curative effect of traditional Chinese and western medicine in the treatment of functional delayed gastric emptying after major gastrectomy.Methods From March 2010 to March 2015, a total of 128 cases with gastric dissection of functional delayed gastric emptying after major gastrectomy in the Affiliated Hospital of Chengdu University of TCM were divided into control group ( n＝64) and observation group ( n ＝64) based on a random number table.The control group was given western medicine,the observation group was given traditional Chinese medicine combined with Western medicine.The clinical efficacy,gastric function recovery time of the two groups were compared.Results The gastric function recovery time in the observation group was (14.12 ±1.36)d, which was significantly shorter than (20.56 ±2.10)d in the control group,and the difference was statistically significant (P＜0.05).The total effective rate of the observation group (87.50%) was significantly higher than that of the control group (68.75%),and the difference was statistically significant ( P＜0.05).Conclusion Comprehensive therapy combined with western and Chinese medicine in the treatment of functional delayed gastric emptying after major gastrectomy has significant clinical effect ,which is worthy of further application.

Expression of the reporter LacZ driven by human dentin sialophosphoprotein promoter in human dental mesenchymal cells / 生物工程学报

The expression of dentin sialophosphoprotein (DSPP) is the marker for cells differentiated into odontoblasts. This study attempted to analyze the DSPP promoter and build the reporter LacZ expression system driven by this promoter, which allows convenient and quick detection of odontoblast cells. First, we separated the human dental mesenchymal cells in which the expression of DSPP can be effectively induced by dexamethasone. Second, four 5' flanking regions of human DSPP gene (- 4 000-+54, -2 500-+54, -1 447-+54 and -1 027-+54) were analyzed, the results showed that the highest promoter activity lied in the -2 500-+54 region. The promoter activity is reduced when the 5' flanking region was extended from -2 500 to -4 000 bp upstream from the transcription start site; The promoter activity are also decreased when the 5' flanking regions were shorted from -2 500 to -1 447 bp and from -1 447 to -1 027 bp, indicating that potential suppresser elements are lied in the region between -4 000 and -2 500 bp and potential activator elements are lied in the region between -2 500 and -1 027 bp. Then we constructed the lentiviral report vector phDSPP-LacZ containing the -2 500-+ 54 promoter region in front of the LacZ gene. The expression of LacZ was detected using X-Gal staining in both human dental mesenchymal cells and immortalized human dental mesenchymal cells infected with phDSPP-LacZ. The phDSPP-LacZ lentiviral vector may provide a more convenient method to detect the expression of DSPP in human odontogenic differentiation, tooth development and tooth regeneration studies.

Proved cases of drug-induced severe liver damage treated by Professor LI Ping / 中华中医药杂志

Liver-injury caused by chemicals is a common question in modern clinic. Professor LI Ping proposes ‘drug yellow’theory to guide TCM differential treatment. In this paper, the author will analyze the chemicals by the proved cases of drug-induced severe liver damage.