Assuntos
Anti-Infecciosos/farmacologia , Infecções por Chlamydia/veterinária , Chlamydia/efeitos dos fármacos , Rifamicinas/farmacologia , Doenças dos Suínos/tratamento farmacológico , Animais , Células Cultivadas , Infecções por Chlamydia/tratamento farmacológico , Farmacorresistência Bacteriana , Técnicas In Vitro , Rifaximina , SuínosRESUMO
In 2008, 50 samples (BAL), coming from hospital patients, with acute respiratory symptoms have been investigated using two real-time PCR methods: one assay for the single detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae DNA and one commercially available real-time duplex PCR assay for the detection of C. pneumoniae and M. pneumoniae DNA. Both techniques used here showed compliant results, with 100% concordance for detection of C. pneumoniae and 98% for detection of M. pneumoniae. The positive results obtained agreed with the clinical suspicion of such infections in some cases and with the presence of IgM specific for C. pneumoniae and M. pneumoniae in all cases of acute infection.
Assuntos
Chlamydophila pneumoniae/isolamento & purificação , Mycoplasma pneumoniae/isolamento & purificação , Pneumonia Bacteriana/diagnóstico , Pneumonia por Mycoplasma/diagnóstico , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Testes Sorológicos/métodos , Líquido da Lavagem Broncoalveolar/microbiologia , Chlamydophila pneumoniae/genética , Chlamydophila pneumoniae/imunologia , Infecções Comunitárias Adquiridas/sangue , Infecções Comunitárias Adquiridas/diagnóstico , Infecções Comunitárias Adquiridas/microbiologia , DNA Bacteriano/análise , Humanos , Mycoplasma pneumoniae/genética , Mycoplasma pneumoniae/imunologia , Pneumonia Bacteriana/sangue , Pneumonia Bacteriana/microbiologia , Pneumonia por Mycoplasma/sangue , Pneumonia por Mycoplasma/microbiologia , Reprodutibilidade dos TestesAssuntos
Anticorpos Antibacterianos/sangue , Infecções por Chlamydia/veterinária , Chlamydia/imunologia , Imunofluorescência/veterinária , Testes de Neutralização/veterinária , Doenças dos Suínos/imunologia , Aborto Animal/microbiologia , Animais , Chlamydia/isolamento & purificação , Infecções por Chlamydia/imunologia , Conjuntivite Bacteriana/veterinária , Humanos , Itália , Suínos , Doenças dos Suínos/microbiologiaRESUMO
The distribution of Chlamydia trachomatis serovars among 157 heterosexual male patients with urethritis and the presence of coinfections with other sexually transmitted infections were studied. One hundred seventeen (74.5%) patients, with a mean age of 33.7 years, were Italians, whereas 40 (25.5%) were immigrants coming from eastern European countries, Africa, and South America. All the immigrants and 82 (70.0%) Italian patients reported sex with prostitutes. Out of 157 patients, 73 (46.5%) were found positive for C. trachomatis in urethral secretions and eight different C. trachomatis serovars were identified. The most common serovars were E (n = 18; 24.7%), D (n = 15; 20.5%), G (n = 14;19.2%), and F (n = 12; 16.4%). The sequencing data showed a high degree of conservation of the omp1 gene. Thirty-six (46.7%) out of the 73 C. trachomatis-positive patients were coinfected with another sexually transmitted infection. The most common coinfection was gonorrhoea detected in 22 (30.1%) patients, followed by condyloma in eight (8.2%) patients, syphilis in five (6.8%), and HIV in three (4.1%).
