The prognostic value of miR-487a in clear cell renal cell carcinoma and its influence on cell biological behavior

Objectives: Clear cell renal cell carcinoma (ccRCC) is a type of kidney cancer that has a poor prognosis. microRNA-487a (miR487a) plays a role in the prognosis of gastric cancer, liver cancer, and other cancers. The purpose of this study is to explore therole of miR-487a in the generation and progression of ccRCC.Materials and Methods: The RT-qPCR technology was used to detect the expression levels of miR-487a in ccRCC tissues and celllines. The association between miR-487a and clinical-pathological characteristics of patients was analyzed using the chi-squaretest. Kaplan-Meier survival analysis and Cox regression analysis were used to analyze the prognostic significance of miR-487ain ccRCC. CCK-8 and Transwell assays were used to analyze the influences of miR-487a on cell proliferation, migration, andinvasion.Results: miR-487a was significantly up-regulated in ccRCC tissues and cell lines. The high expression of miR-487a is related tolymph node metastasis and TNM staging and may be used as an independent prognostic factor related to lower overall survivaland disease-free survival rate. Increased expression of miR-487a accelerated the proliferation, migration, and invasion of ccRCCcells.Conclusion: The enhanced expression of miR-487a was related to the prognosis of ccRCC, and it also facilitated cell proliferation,migration, and invasion. (AU)

Blood transfusion strategies in patients on extracorporeal membrane oxygenation / 中国输血杂志

【Objective】 To analyze the blood transfusion strategies in extracorporeal membrane oxygenation (ECMO) recievers in our hospital, so as to explore the clinical application and efficacy of blood transfusion in patients on ECMO. 【Methods】 Data from patients on ECMO treatment in our hospital from October 2017 to October 2021 was collected. The blood transfusion data and coagulation monitoring indexes during different ECMO modes were analyzed, and the efficacy of blood transfusion was evaluated. 【Results】 No difference in the number of blood transfusions was noticed by ECMO treatment modes.The transfusion units of red blood cells, plasma and platelets in VA mode were (28.35±14.60) U, (7 367.78±5 194.33) mL and (7.04±5.10) therapeutic volumes, which were higher than those in VV mode, i. e. (18.67±21.50) U, (4 836.67±6 640.50) mL and (3.60±7.47) therapeutic dose, respectively.In VA-ECMO mode, the Hb level and platelet count before ECMO treatment were (126.44±23.9) g/L and (223.84±67.62) × 109/L, which were significantly higher than those after treatment (91.02±21.48) g/L and (172.86±127.73)×109/L.In VV-ECMO mode, the APTT before ECMO treatment was (35.28±8.73) s, which was shorter than that after treatment (41.96±13.69) s. The levels of fibrinogen, Hb and platelet count were (3.80±1.85) g/L, (123.81±33.77) g/L and (175.72±98.91)×109/L, which were significantly higher than the levels after treatment (2.78±1.08) g/L, (92.31±17.38) g/L and (125.31±98.14)×109/L, respectively. 【Conclusion】 There are differences in the amount of blood transfusion among different modes of ECMO treatment. As blood transfusion is a necessary support to ensure ECMO treatment, the monitoring of coagulation index is conducive to reduce blood transfusion, improve the efficiency of blood transfusion and benefit to patient safety.

Application of Internet of Things in clinical blood management based on RFID technology / 中国输血杂志

【Objective】 To explore and evaluate the application of blood intelligent management platform (scheme) based on the Internet of Things(IoT)in the clinical blood management for hospitals. 【Methods】 Based on radio frequency identification technology (RFID), smart blood refrigerators, IoT blood shipping containers, automated blood bank systems, smart blood management software, etc. were developed and integrated as an IoT blood intelligent management platform (scheme). The blood storage, management software and hardware systems were organically combined, and the blood storage equipment was moved forward to the clinical departments to solve the concerns of clinicians. 【Results】 The in-depth integration of IoT technology, RFID and refrigeration technology has built an RFID-based IoT blood management solution, which integrates blood storage, transfusion, and quality control management, also realizes the entire process of supervision and traceability of clinical blood transfusion. The forward movement of blood bank to the clinical departments and the implementation of electronic cross-matching streamlined and optimized the clinical blood flow. The waiting time of patient′s for blood transfusion was shortened from (40±10) min to less than 2 min. The whole process of cold chain logistics ensured the storage quality of blood products issued, so that the clinical departments can return the untransfused blood and Blood Transfusion Department can reissue it to other hospitals. 【Conclusion】 IoT blood intelligent management based on RFID realizes the intelligent management of clinical blood transfusion and blood information traceability. The forward movement of blood bank to the clinical departments improves the efficiency of clinical blood transfusion, avoids the waste of blood source, and ensures the safety of blood transfusion. It is worth promoting in the whole process of blood transfusion.

Efficacy Observation of Cyclophosphamide,Leflunomide Sequentially Combined with Prednisone in the Treatment of Membranous Nephropathy in StageⅡ / 中国药房

OBJECTIVE:To observe the efficacy and safety of cyclophosphamide,leflunomide sequentially combined with prednisone in the treatment of membranous nephropathy in stage Ⅱ. METHODS:72 patients with membranous nephropathy in stageⅡwere randomly divided into group A(24 cases),B(24 cases)and C(24 cases). All patients were given angiotensin convert-ing enzyme inhibitor (ACEI) Benazepril hydrochloride tablet,warfarin,dipyridamole,calcium and other conventional treatment. Based on it,group A was orally given 50 mg Compound cyclophosphamide tablet,twice a day,for continuous 3 months+1.0 mg/(kg·d)Prednisone tablet,it was reduced 5 mg every 2 weeks after 2 months,then it reduced 2.5 mg every 2 weeks when 30 mg/day,and then it maintained 4-6 months when 10 mg/day,it lasted for 12 months. Group B was given 50 mg Leflunomide tablet,it changed as 20 mg/day after 2 d,and for continuous 6 months+Prednisone tablet(the same dosage and usage as group A). Group C was given Compound cyclophosphamide tablet(the same dosage and usage as group A)was orally given in 1-3 months,and Leflu-nomide tablet(the same dosage and usage as group B)in 4-9 months Prednisone tablet(the same dosage and usage as group A). Clinical efficacy,24 h urinary protein,albumin,total cholesterol,serum creatinine,alanine aminotransferase before and after 3, 6,9 and 12 months and incidence of adverse reactions in all groups were observed. RESULTS:Before treatment,there were no significant differences in the 24 h urinary protein,albumin,total cholesterol,serum creatinine and alanine aminotransferase among all groups(P>0.05). After treatment,24 h urinary protein,total cholesterol,serum creatinine and alanine aminotransferase in all groups were significantly lower than before,and they gradually decreased by treatment time,24 h urinary protein in group C was lower than group A and B,albumin was significantly higher than before and gradually increased by treatment time,and group C was higher than group A and B,the differences were statistically significant(P0.05). The total effective rate in group C was significantly higher than group A and B,the dif-ference was statistically significant (P0.05). And there was no significant difference in the incidence of adverse reactions among 3 groups (P>0.05). CONCLUSIONS:Based on the conventional treatment,the efficacy of cyclophosphamide,leflunomide sequentially combined with glucocorticoid is superior to cyclophosphamide or leflunomide alone in the treatment of membranous nephropathy in stageⅡ,with similar safety.

Current evidence on the cytotoxic T-lymphocyte antigen 4 + 49G > A polymorphism and digestive system cancer risks: a meta-analysis involving 11,923 subjects.

Cytotoxic T-lymphocyte antigen (CTLA-4) plays an important role in downregulating T cell activation and proliferation. The CTLA-4 + 49G > A polymorphism is one of the most commonly studied polymorphisms in this gene due to its association with many cancer types, but the association between CTLA-4 + 49G > A polymorphism and digestive system cancer risks remain inconclusive. An updated meta-analysis based on 17 independent case-control studies consisting of 5176 cancer patients and 6747 controls was performed to address this association. Overall, there was no statistically increased risk of digestive system cancers in every genetic comparison. In subgroup analysis, this polymorphism was significantly linked to higher risks for pancreatic cancer (GG vs. AA, OR = 1.976, 95% CI = 1.496-2.611; GA vs. AA, OR = 1.433, 95% CI = 1.093-1.879; GG/GA vs. AA, OR = 1.668, 95% CI = 1.286-2.164; GG vs. GA/AA, OR = 1.502, 95% CI = 1.098-2.054; G vs. A, OR = 1.394, 95% CI = 1.098-1.770). We also observed increased susceptibility of hepatocellular cell carcinoma in homozygote comparison (OR = 1.433, 95% CI = 1.100-1.866) and dominant model (OR = 1.360, 95% CI = 1.059-1.746). According to the source of controls, significant effects were only observed in hospital-based studies (GA/AA vs. GG, OR = 1.257, 95% CI = 1.129-1.399). In the stratified analysis by ethnicity, no significantly increased risks were found in either Asian or Caucasian. Our findings suggest that the CTLA-4 + 49G > A polymorphism may be associated with the risk of pancreatic cancer and hepatocellular cell carcinoma.

Rapid preparation of antiserum against influenza virus (H7N9) hemagglutinin for single radial immunodiffusion assay / 中华微生物学和免疫学杂志

Objective To establish a method for rapid preparation of antiserum against influenza virus (H7N9) hemagglutinin,and to study the possibility of using it in single radial immunodiffusion (SRID) assay for quantitative detection of antigen in H7N9 influenza vaccine.Methods Hemagglutinin proteins expressed in eukaryotic cells were used to immunize sheep.Serum samples were collected to detect antibody titers by ELISA and double immunodiffusion assay.Different concentrations of antiserum were used in SRID assay to get the optimized concentration.Results After 4 times of immunization,the antiserum titers achieved 1 ∶ 1 000 000 and 1 ∶ 32 as indicated by ELISA and double immunodiffusion assay,respectively.The antiserum could form a clear precipitation line in SRID assay.The detection of antigen in the range of 10 to 40 μg/ml showed good linearity in the standard curve.The antigen titers in six batches of H7N9 vaccine detected by this SRID assay were identical with those by SDS-PAGE assay.Conclusion The antiserum against H7N9 hemagglutinin for SRID assay was developed successfully,and could be used as a reagent for the quantitative detection of antigen in H7N9 influenza vaccine.

Investigation and analysis of influence of social support and coping style on depression mood of infertile patients / 中国实用护理杂志

Objective This study aims to explore the social support,coping style on depressive mood of infertile patients.Methods 165 infertility patients were collected as the research object,and they were investigated with the self-rating depression scale,social support scale and coping style questionnaire.Results 34.55％,48.48％ of the patients with education background of junior middle school,high school appeared depression.Subjective support and positive coping had significant positive correlation.The availability of support and positive coping had significant positive correlation.Positive coping and depression had significant negative correlation,negative coping and depression had significant positive correlation.Educational level and depression demonstrated inverted U shape relationship; coping style had direct effect and indirect effect on infertile female patients with depression.Conclusions Use of active coping without negative coping can reduce depressive mood,improve patients' social support in order to reduce mental stress level.

Quantification of HA in H7N9 influenza vaccine using heterogeneous antiserum of the same HA sub-type virus / 中华微生物学和免疫学杂志

Objective To study the possibility of using heterogeneous antiserum in single radial immunodiffusion (SRID) for quantitative detection of HA in H7N9 influenza vaccine product when H7N9-specific antiserum is not available in order to establish a testing method for the detection of H 7N9 antigen in any urgent situation.Methods Antisera specific for H7N1, H7N2, H7N3 and H7N7 were obtained from NIBSC and used for SRID assay .Amino acid sequences of hemagglutinins were comparatively analyzed be-tween H7N9 virus and other viruses used to prepare heterogeneous antiserum .The titers of antisera against H7N9 and their homogenous antigens were detected by double immunodiffusion method .Based on the results of homology analysis and cross-reaction, a suitable antiserum was selected and its applicability was further validated by the SRID assay using H7N9 antigen.Results Influenza A virus subtype H7N3 that used for preparation of 07/278 antiserum showed the highest HA homology with H7N9 (97.14%).The titer of 07/278 antiserum against H7N9 antigen was 1 ∶8 as detected by double immunodiffusion assay .The H7N9 anti-gen and the 07/278 antiserum could form a clear precipitation line in SRID assay .The detection of H7N9 antigen in the range of 10 to 40μg/ml showed a good linearity in the standard curve .Conclusion The 07/278 antiserum from NIBSC can be used as an alternative reagent for the quantitative detection of hemaggluti -nin in H7N9 influenza virus vaccine .

Quality analysis of 2009 pandemic influenza A(H1N1) vaccines / 中华微生物学和免疫学杂志

Objective To analyze the laboratory testing data of 2009 pandemic influenza A (H1N1) vaccines during lot release procedure, thus to know the overall quality status of this vaccines.Methods National Institutes for Food and Drug Control(NIFDC) carried out the laboratory test according to the specifications of each manufacture, and the results was analyzed and compared between manufacturer and NIFDC. Results 99.8% of vaccines batches were released by NIFDC, haemagglutinin contents were between 90% to 103 % of labeled values, and testing results slightly differ between manufactures and NIFDC,other items related to safety were all meet specifications. Conclusion The quality of H1N1 vaccines in China were satisfying, the lot release and independent test by NIFDC play important roles to ensure the vaccines' quality.

Establishment and identification of universal antibody against all subtypes of the influenza A viral hemagglutinins / 中华微生物学和免疫学杂志

Objective To establish the universal antibody against all subtypes of the influenza A viral hemagglutinins(HA). Methods All HA sequences from influenza viruses in common domains were analyzed to search for the most conservative re,on of the HA gene. The peptides conjugated to carrier proteins were used to immunize rabbits in order to obtain high titer antibody. Results The most conservative region among all influenza viruses HA genes were 14 amino acids located at the N-terminal of HA2. The antibody titer reached to 1 : 80 000 after 4 injections of the coupled peptides. The achieved antibody was demonstrated by Western blot to bind HA proteins of influenza virus subtypes H1-H13 specifically. Conclusion The universal antibody has been successfully established by immunizing the rabbits with most conservative peptides of HA protein coupled to carrier protein.

Protective effects of fluvastatin on cyclosporine A-induced nephrotoxicity in rat fibroblast / 中华肾脏病杂志

Objective To investigate the protective effects of fluvastatin(Flu) on rat renal fibroblasts proliferation and cytokines expression induced by cyclosperine A (CsA). Methods The fibroblasts were cultured with CsA or with CsA plus fluvastatin. The cellular proliferation was determined by MTT colorimetry. The mRNA expression of transforming growth factor β1(TGF-[β1), connetive tissue growth factor (CTGF) and c-fos was detected by BT-RCB. The protein level of flbronecfin (FN) was measured by Western blotting. Results CsA inhibited the proliferation of fibroblasts in dose- and time-dependent manner (P＜0.05). Treatment with Flu accelerated the suppression of fibroblasts resulted from CsA (P＜0.O1). CsA stimulated the expression of TGF-β1, CTGF, c-fos and FN compared with control group(P＜0.05), which could be down-regulated by Flu (P＜0.05). Conclusion Fluvastatin may relieve CsA-induced nephrotoxicity in rat fibroblasts.

Management of Nosocomial Infection Through Network / 中华医院感染学杂志

OBJECTIVE To understand the knowledge about nosocomial infection of the personal. METHODS A questionnaire investigation was carried out by the hospital local area network(LAN). RESULTS A total of 202 questionnaires were sent in during one month, and statistic result could be looked up at any moment. CONCLUSIONS Questionnaire investigation via LAN can find the shortcomings of the nosocomial infection management correctly and quickly.

Expression of proteins in mouse thymocytes induced by low dose X-rays and their biological activity / 中国病理生理杂志

AIM: To observe the expression of proteins induced by whole-body irradiation in mice with low dose X-rays and their biological activity. METHODS: To analyse the expression of protein induced by whole-body irradiation with 75mGy X-rays and sham-irradiation, the method of gel filtration with Sephadex G-100 and HPLC were used. The biological activity of protein expressed in thymocytes was observed by mouse splenocyte proliferation and chromosome aberration of human peripheral blood lymphocytes. RESULTS: HPLC analysis showed that there was a marked decrease in expression of 24.5 kD protein molecule in the fractions of thymocyte extract in comparison with the corresponding fractions from the sham-irradiated control mice. These protein fractions from the thymocytes of the irradiated mice at the concentration of 6.25 mg/L showed both inhibitory effect on normal T cell proliferation and protective effect on the chromosome damage induced by high dose radiation. CONCLUSION: Down regulation of 24.5 kD protein molecule expression may have implications in the mechanism of immunoenhancement and cytogenetic adaptive response induced by low dose radiation.

Protective effect of rosiglitazone on cyclosporine A-induced nephrotoxicity in rat NRK cells / 中华肾脏病杂志

Objective To investigate the protective effects of rosiglitazone (RSG) on normal rat kidney cells (NRK) damaged by cyclosporine A(CsA). Methods The NRK cells were cultured with CsA or with CsA plus rosiglitazone. The cellular proliferation was determined by MTT colorimetry. The mRNA expression of TGF-?1 and PPAR? was detected by RT-RCR. Protein levels of PPAR?, p-ERK,FN and AT1R were examined by Western blotting. The level of TGF-?1 in the supernatants was measured by enzyme-linked immunosorbant assay (ELISA). Results CsA inhibited the proliferation of NRK cells in dose and time dependent manner (P

Strengthen the Humanities Education for Hospital Political Affairs Staff to Enhance Their Qualification——An Investigation of Humanities Qualification of Hospital Political Affairs Staff in Yancheng Manicipal Hospitals / 中国医学伦理学

A sample investigation is conducted among 109 political affairs staff from different scales of hospitals in Yancheng city.By analyzing the investigation data,this paper points out there is a lack of humanities qualification among hospital political affairs staff.Some countermeasures are also come up with as follows.The enhancement of humanities knowledge should be the first step to improve qualification of political affairs staff.Political affairs staff should take a positive role in adapting to the challenges of information economics.The political affairs work should be initiated with humanities culture in order to keep pace with social progress.The mutual improvement in humanities education and political affairs work lies a solid foundation for the construction of a harmonious physician-patient relationship.

Clinical Observation on Rheumatoid Arthritis Patients at Active stage Treated by Western and Chinese Medicines: 100 Cases Report / 中医杂志

Objective To observe the effect of combination of Chinese and western medicines on rheumatoid arthritis(RA) at active stage. Methods Totally 150 cases of RA at active stage were randomized into two groups. The control group was given western medicines including Leflunomide, Sulfasalazine suppository and Celecoxib. The treatment group (100 cases) was given Chinese medicines Sihua Siteng Sichong Decoction (Decoction of four flowers, flour stems and four worms) in addition to those western medicines. The course for two groups was 3 months respectively. Clinical symptoms,ESR,CRP,RF and Immuno hemoglobin before and after treatment were observed. Results Of the 100 cases in the treatment group, 30 cases were remarkably effective, 43 cases improved, 20 cases effective, and 7 cases ineffective. The total effective rate was 93%. Of the 50 cases in the control group, 8 cases were remarkably effective, 20 cases improved, 12 cases effective, and 10 cases ineffective. The total effective rate was 80%. The difference between the two groups was significant (P