RESUMO
Expression of total T- and B-population, T-subpopulation lymphocyte receptors and secretion of IgM, IgG and IgA were measured in 112 patients with diffuse purulent peritonitis and 81 healthy donors. Expression of receptors was correlated with the disease severity identified by leukocytic index of intoxication (LII). The latter appeared to accurately reflect the pattern of immunological impairment. Reduced absolute and proportional values of total pools of T-, B-cells and T-helpers, but elevated ones of T-suppressors and T-amplifiers occurred in LII less than 2.0. In LII greater than 7.0 there was total suppression of all the cellular populations due to deep lymphopenia. At the terminal stage of peritonitis paralysis of T-helper receptors and B-cells went in parallel with high secretion of serum immunoglobulins.
Assuntos
Linfócitos B/imunologia , Infecções Bacterianas/imunologia , Linfopenia/etiologia , Peritonite/imunologia , Linfócitos T/imunologia , Toxemia/imunologia , Infecções Bacterianas/sangue , Humanos , Contagem de Leucócitos , Linfopenia/diagnóstico , Peritonite/sangue , Formação de Roseta , Índice de Gravidade de Doença , Linfócitos T/patologia , Toxemia/etiologiaRESUMO
A method for antiprotease activity measurement based on the use of luminous bacteria luciferase as protein substrate of proteases is suggested. Antiprotease is incubated with protease for 1 to 2 min at 30 degrees C and then it is added to the reaction mixture containing luciferase, NADH: FMN-oxidoreductase and their substrates--myristic aldehyde, FMN and NADH. Biofluorescence is measured in a temperature-controlled cuvette for 1 min. The total time of the measurement is 3 min. The method can be applied both in fine biochemical assays and in medical rapid diagnosis.
Assuntos
Inibidores de Proteases/metabolismo , Humanos , Luciferases , Medições Luminescentes , MétodosAssuntos
Adjuvantes Imunológicos/administração & dosagem , Infecções Estafilocócicas/prevenção & controle , Toxoide Estafilocócico/administração & dosagem , Adjuvantes Imunológicos/farmacologia , Adulto , Animais , Antitoxinas/biossíntese , Terapia Baseada em Transplante de Células e Tecidos , Humanos , Ferro , Óxidos , Coelhos , Ratos , Baço/imunologia , Uracila/administração & dosagem , Uracila/análogos & derivados , Uracila/farmacologiaRESUMO
Unspecific biogenic stimulants (pentoxyl and mummie) accelerated metabolism of nucleic acids and protein in rat liver tissue. After the treatment with the stimulants the rate of lipolysis exceeded that of lipogenesis. Increase in content of lactate was similar if glycogen and glucose-6-phosphate were used as substrates of glycolysis, but it was stimulated 2-3-fold, when glucose was used; the phenomenon appears to be due to activation of hexokinase. As shown by polarographic measurements mitochondrial respiration was increased in all the metabolic states, but increased doses caused an inhibition of phosphorylation apparently due to functional overstrain of mitochondria. Increased doses of the stimulants accelerated also some other metabolic processes studied, but the effects were not dose-dependent. Pentoxyl and mummie apparently increased processes of protein and nuclei acid metabolism and stimulated the energy-providing reactions.