[Decrease in RNA-dependent DNA polymerase activity of recombinant human immunodeficiency virus reverse transcriptase in bidistilled modified water]. / Snizhenie RNK-zavisimoi DNK-polimeraznoi aktivnosti rekombinantnoi obratnoi transkriptazy virusa immunodefitsita cheloveka v bistillirovannoi modifitsirovannoi vode.

It was shown that the exposure of a recombinant reverse transcriptase of human immunodeficiency virus HIV-1 to bidistilled modified water substantially decreases the RNA-dependent DNA polymerase activity of this enzyme.

[Molecular mechanisms of the biological effects of weak magnetic fields. V. Inactivation in vitro of recombinant Rous sarcoma virus reverse transcriptase under the combined action of weak direct and low-frequency alternating magnetic fields, adjusted to the cyclotron resonance of polar amino acid ions]. / Molekuliarnye mekhanizmy biologicheskogo deistviia slabykh magnitnykh polei. V. Inaktivatsiia in vitro rekombinantnoi obratnoi transkriptazy virusa sarcoma Rausa pri kombinirovannom deistvii slabykh postoiannogo i nizkochastonogo peremennogo magnitnykh polei, nastroennykh na tsiklotronnyi rezonans ionov poliarnykh aminokislot.

The effect of weak direct and alternative magnetic fields adjusted to cyclotron frequencies of ions of polar amino acids leads to a functional inactivation of the recombinant reverse transcriptase of Rous sarcoma virus, which is coupled with the proteolysis of this enzyme.

[Molecular mechanisms of biological action of low magnetic fields. IV. Proteolysis of protein-DNAse 1 inhibitor in water phase under combined action of low constant and alternating magnetic fields adjusted to cyclotron resonance of polar amino acid ions]. / Molekuliarnye mekhanizmy biologicheskogo geistviia slabykh magnitnykh polei. IV. Proteoliz belka-ingibitora DNKazy 1 v vodnom rastvore pod vliianiem kombinirovannogo deistviia slabykh postoiannogo i peremennogo nizkochastotnykh magnitnych polei, nastroennykh na tsiklotronnyi resonans ionov poliarnykh aminokislot.

It was shown that combined action of weak magnetic fields adjusted to cyclotronic frequencies of polar amino acids ions, on aqueous solutions of acidic non-histone protein-inhibitor of DNAase 1 led to proteolysis of this inhibitor. The fragments obtained as a result of proteolysis differ from the whole molecule by amino acid composition and the time of elution from column in case of HPLC.

[Polymerase and immunologic activity of reverse transcriptase of the HIV-1 virus, isolated from Escherichia coli]. / Polimeraznaia i immunologicheskaia aktivnost' rekombinantnoi obratnoi transkriptazy virusa HIV-1, vydelennoi iz Escherichia coli.

The recombinant reverse transcriptase of HIV-1 virus has been isolated from Escherichia coli cells transformed by the plasmid pRT40 DNA. The 103 Kd protein produced by these cells is shown to be processed to proteins with lower molecular masses by the reverse transcriptases own protease activity as well as Escherichia coli proteases. The resulting 103-66 Kd proteins possess the polymerase activity while 51 Kd and smaller proteins are lacking the activity. The 66 and 51 Kd reverse transcriptase fragments demonstrate the positive immunological reaction with the human blood serum from the people possessing antibodies to HIV-1 virus. The recombinant reverse transcriptase of HIV-1 produced by Escherichia coli cells is shown to be useful in AIDS diagnosis in humans.

[Molecular DNA-DNA hybridization in Pseudomonas fluorescens and Pseudomonas putida]. / Molekuliarnaia gibridizatsiia DNK-DNK u Pseudomonas fluorescens i Pseudomonas putida.

Taxonomic analysis was carried out and DNA-DNA homology studied in cultures of subgroups formed by numerical classification of 124 Pseudomonas fluorescens and P. putida strains. The GC content in the DNA of strains belonging to P. fluorescens subgroups varied from 61.2 to 64.5%, and their DNA-DNA homology with the type strain P. fluorescens ATCC 13525 was 24 to 83%. The lowest genome relatedness with the type culture was found in biotype C of P. fluorescens and P. aureofaciens strains. The GC content in the DNA of strains belonging to two subgroups formed by numerical classification of P. putida varied from 63.8 to 65.0%, and the homology of their DNA with the DNA of the type strain of P. fluorescens was 0 to 10% (for strains of the first and second subgroups, respectively).

[Differentiation of bacteria of the genus Aeromonas from other representatives of the Vibrionaceae family on the basis of their DNA]. / Differentsiatsiia bakterii roda Aeromonas ot drugikh predstavitelei semeistva Vibrionaceae na osnove izucheniia ikh DNK.

By comparing the data on the nucleotide composition of DNA, and the phenotypic characteristics, most of the representatives of the genus Aeromonas were clearly differentiated from NAG vibrios isolated in the process of sanitary control of the environment. At the same time some microorganisms with the Aeromonas phenotype, but having a different DNA structure were detected. The use of the method of molecular DNA hybridization indicated that these bacteria were the taxons analogous to the genus Aeromonas.