Combination of nitrate and sodium nitroprusside dosing for sulfide control with low carbon source loss in sewer biofilm reactors.

Nitrate has been widely used in sewer systems for sulfide control. However, significant chemical consumption and the loss of carbon source were observed in previous studies. To find a feasible and cost-effective control strategy of the sulfide control, the effect of nitrate combined with sodium nitroprusside (SNP) dosage strategy was tested in lab-scale sewer biofilm reactors. Results showed that nitrate and SNP were strongly synergistic, with 30 mg N/L nitrate and 20 mg/L SNP being sufficient for sulfide control in this study. While large amount of nitrate alone (100 mg N/L) is required to achieve the same sulfide control effectiveness. Meanwhile, the nitrate combined with SNP could reduce the organic carbon source loss by 80%. Additionally, the high-throughput sequencing results showed that the relative abundance of autotrophic, nitrate reducing-sulfide oxidizing bacteria genera (a-NR-SOB) such as Arcobacter and Sulfurimonas was increased by around 18%, while the heterotrophic, nitrate-reducing bacteria (hNRB) such as Thauera was substantially reduced. It demonstrated that the sulfide control was mainly due to the a-NR-SOB activity under the nitrate and SNP dosing strategy. The microbial functional prediction further revealed that nitrate and SNP promoted the dissimilatory nitrate reduction process which utilizes sulfide as an effective electron donor. Moreover, economic assessment indicated that using the combination of nitrate and SNP for sulfide control in sewers would lower the chemical costs by approximately 35% compared with only nitrate addition.

Case study of H2S release and transport in a trunk sewer with drops.

Field work was performed to investigate the release of hydrogen sulphide (H2S) and its transport in the sewer trunk with drops in the Bonnie Doon area in Edmonton, Alberta, Canada, in order to develop a proper odor control strategy. The liquid sulfide concentration in the upstream trunk was low (less than 1.0 mg/L), and no H2S gas was detected in the head space under this low concentration. However, high H2S gas concentration was detected in the middle reach of the trunk due to the stripping effect of the three drops (2.7 m, 5.2 m and 2.0 m) along the trunk. The released H2S at drops was then transported in the sewer system and emitted at various locations and caused odor concerns. These drops played an important role in H2S release, and the overall H2S mass transfer coefficient at drops was much higher than that in normal gravity sewers. The overall oxygen and H2S mass transfer coefficient (KLa) was estimated to be around 200 h-1 and 300 h-1 at the first two drops, respectively. Field sampling of biofilm indicates that Desulfomicrobium was identified as the sulfate-reducing bacteria (SRB) responsible for sulfide generation in sewer wall biofilm and Thiobacillus was the only predominant member in manhole wall biofilm contributing to sewer manhole corrosion.

Experimental Investigation and Modeling of the Transformation of Illicit Drugs in a Pilot-Scale Sewer System.

In-sewer stability of illicit drug biomarkers has been evaluated by several reactor-based studies, but less has been done in sewer pipes. Experiments conducted in sewer pipes have advantages over lab-scale reactors in providing more realistic biomarker stability due to the flow and biological dynamics. This study assessed the transportation and transformation of seven illicit drug biomarker compounds in a pilot-scale rising main and a gravity sewer pipe. Biomarkers presented diverse stability patterns in the pilot sewers, based on which a drug transformation model was calibrated. This model was subsequently validated using transformation data sets from the literature, aiming to demonstrate the predictability of the pilot-based transformation coefficients under varying sewer conditions. Furthermore, transformation coefficients for five investigated biomarkers were generated from four studies, and their prediction capabilities under the pilot-sewer conditions were jointly assessed using performance statistics. The transformation model was successful in simulating the in-sewer stability for most illicit drugs. However, further study is required to delineate the sources and pathways for those compounds with potential formations to be simulated in the transformation model. Overall, the transformation model calibrated using the pilot-sewer data is a credible tool for the application of wastewater-based epidemiology.

Systematic evaluation of biomarker stability in pilot scale sewer pipes.

Transformation of biomarkers (or their stability) during sewer transport is an important issue for wastewater-based epidemiology (WBE). Most studies so far have been conducted in the laboratory, which usually employed unrealistic conditions. In the present study, we utilized a pilot sewer system including a gravity pipe and a rising main pipe to investigate the fate of 24 pharmaceutical biomarkers. A programmable logic controller was used to control and monitor the system including sewer operational conditions and wastewater properties. Sequential samples were collected that can represent hydraulic retention time (HRT) of up to 8 h in a rising main and 4 h in a gravity sewer. Wastewater parameters and biomarker concentrations were analysed to evaluate the stability and transformation kinetics. The wastewater parameters of the pilot system were close to the conditions of real sewers. The findings of biomarker transformation were also close to real sewer data with seventeen biomarkers reported as stable while buprenorphine, caffeine, ethyl-sulfate, methadone, paracetamol, paraxanthine and salicylic acid degraded to variable extents. Both zero-order and first-order kinetics were used to model the degradation of unstable biomarkers and interestingly the goodness of fit R2 for the zero-order model was higher than the first-order model for all unstable biomarkers in the rising main. The pilot sewer system simulates more realistic conditions than benchtop laboratory setups and may provide a more accurate approach for assessing the in-sewer transformation kinetics and stability of biomarkers.