HPLC-OPLC-MS investigation of change of formaldehyde and its generators in human teeth of different physiological stage.

Taking into consideration the unquestionable intracellular occurrence of formaldehyde (HCHO) and its generators in cells of plant, animal and human organisms as well as in body fluids it was resolved to determine their levels in hard tissues of physiologically and pathologically changed teeth. The aim of the work was to determine the relationship between the level of HCHO and the levels of its generators in pathologically changed teeth, mainly carietic teeth as tooth caries is still a serious and commonly occurring problem. The occurrence of HCHO (captured as its dimedone adduct) and some of its potential generators was demonstrated in the hard tissues of healthy and pathological human teeth by means of OPLC, HPLC and MS analyses. It was established that the measurable level of HCHO was increased in the carietic teeth in comparison with healthy ones. In the case of paradontic tooth sample, a dramatic increase of HCHO was observed and at the same time the level of betaines was decreased considerably. The obtained results give a new insight into the pathology of hard tissues of teeth in strong correlation with the phases of stress syndrome.

Measurement of formaldehyde as dimedone adduct and potential formaldehyde precursors in hard tissues of human teeth by overpressured layer chromatography.

Simple, automatic overpressured layer chromatographic methods for the parallel determination of endogenous formaldehyde in the form of dimedone adduct and potential formaldehyde generators (betaines) in hard tissues of human teeth are described together with an efficient densitometric evaluation. These simple procedures involve the special preparation of teeth and extraction of formaldehyde, of different binding force in teeth, with methanol containing dimedone, and the isolation of betaines with an aqueous solution of methanol.

The increase of formaldehyde level in some rare pathological cases of teeth determined with the use of quantitative TLC.

In our previous works we proved that formaldehyde (HCHO) level in hard tissues of teeth could depend on their physiological state. In the current paper we presented the results of HCHO determinations in different dental pathologies, mainly the ones rarely encountered in dental practice, e.g. in the case of reinclusion. The determination of HCHO in the form of dimedone was performed by means of quantitative TLC. The obtained results were compared with HCHO levels in hard tissues of teeth presenting pathological changes. It proved that the highest HCHO level was found in reincluded teeth while it was lower in retained teeth, that is the ones which are not subjected to stress factors present in the mouth. The obtained results can constitute a contribution to the problems of dental pathologies, mainly caries which is a very common problem.

Comparative quantitative chromatographic determination of formaldehyde in different groups of physiological and pathological hard tissues of teeth.

Taking into consideration that HCHO level in cells of plant, animal and human tissues as well as in body fluids depends from physiological state of an organism in the current study it was decided to find out if there are changes of HCHO level in different physiological and pathological hard tissues of teeth. The obtained results showed in all 4 groups of teeth separately analysed that there were some regularities in the level of HCHO as far as similar physiological or pathological states are concerned. This was best seen when comparing the obtained results with mean HCHO level of the studied groups of teeth.