The application of polystyrene waveguides to protein adsorption investigations.

The possibility of detecting peroxidase adsorption on the surface of a planar polystyrene waveguide has been studied using light of the wavelength adsorbed by the protein molecules. When a single-mode optical waveguide was employed, maximal protein sensitivity was obtained when the thickness of waveguide layer was about 1.5-times greater than the cutoff thickness. Adsorption kinetics of peroxidase on the surface of polystyrene was studied with single-mode waveguides. A minimum surface concentration of 0.1 mg/m2 was observed experimentally.

[The use of surface electromagnetic waves of IR range for recording the protein sorption on the phase boundary and optic biosensors on this basis]. / Ispol'zovanie poverkhnostnykh élektromagnitnykh voln IK-diapazona dlia registratsii sorbtsii belkov na granitsu razdela faz i optobiosensory na étoi osnove.

Monomolecular protein films adsorbed on the copper and aluminium surfaces were studied by the IR surface electromagnetic waves laser spectroscopy. The thickness of the protein films was measured. A model of IR biosensors was proposed and its sensitivity limits were estimated.