Morpho-Physiological Traits and Oil Quality in Drought-Tolerant Raphanus sativus L. Used for Biofuel Production.

Raphanus sativus L. is a potential source of raw material for biodiesel fuel due to the high oil content in its grains. In Brazil, this species is cultivated in the low rainfall off-season, which limits the productivity of the crop. The present study investigated the effects of water restriction on the physiological and biochemical responses, production components, and oil quality of R. sativus at different development stages. The treatments consisted of 100% water replacement (control), 66%, and 33% of field capacity during the phenological stages of vegetative growth, flowering, and grain filling. We evaluated characteristics of water relations, gas exchange, chlorophyll a fluorescence, chloroplast pigment, proline, and sugar content. The production components and chemical properties of the oil were also determined at the end of the harvest cycle. Drought tolerance of R. sativus was found to be mediated primarily during the vegetative growth stage by changes in photosynthetic metabolism, stability of photochemical efficiency, increased proline concentrations, and maintenance of tissue hydration. Grain filling was most sensitive to water limitation and showed a reduction in yield and oil content. However, the chemical composition of the oil was not altered by the water deficit. Our data suggest that R. sativus is a drought-tolerant species.

Involvement of glutathione and glutathione metabolizing enzymes in Pistia stratiotes tolerance to arsenite.

Glutathione is essential for plant tolerance to arsenic but few studies have focused on the coordination between the enzymes involved in its metabolism. We exposed Pistia stratiotes to four treatments (control, 5, 10 and 20 µM AsIII) for 24 h to evaluate the role of glutathione metabolism in arsenic response and determined the arsenic uptake, growth, membrane integrity, glutathione concentration and enzyme activities (γ-glutamyl-cysteine synthetase, glutathione reductase, glutathione peroxidase, and glutathione-S-transferase). Despite absorbing high concentrations of AsIII, plants maintained growth and cell membrane integrity when exposed to concentrations of up to 10 µM AsIII. The maintenance of these parameters involved glutathione concentration increase due to an increase in its biosynthetic pathway (higher γ-glutamyl-cysteine synthetase). In addition, an increase in the activity of glutathione reductase, glutathione peroxidase and glutathione-S-transferase also contributed to the conserve the cellular homeostasis. However, at the concentration of 20 µM AsIII, the high toxicity of AsIII affected glutathione concentration and glutathione metabolizing enzymes activities, which resulted in drastic decrease in growth and damage to cell membranes. These results showed that not only the glutathione concentration but also the coordination of the enzymes involved in the synthesis, oxidation and reduction pathways of glutathione is essential for AsIII tolerance.