Autistic spectrum disorder children and adolescents' oral health characteristics - scoping review.

This study aimed to conduct a scoping review to know children and adolescents' main oral health concerns with Autism Spectrum Disorder (ASD) and thus identify any oral characteristic that stands out, aiming at preventive and interceptive clinical conduct. A search was performed in the three electronic databases and the gray literature; in September 2021. A total of 562 studies were identified, and after applying the eligibility criteria, 73 articles were included in the synthesis of this scoping review. The mean decayed, lost, and restored teeth index (DMFT/dfmt) ranged from 0 to 12.37; the mean DMFT index (permanent dentition) from 0.06 to 6.2; and the average dmft index (deciduous dentition) ranged from 0.29 to 9.91. The mean simplified oral hygiene index (OHIS) ranged from 0 to 3.4; gingival index (GI) from 0.29 to 2; and plaque index (PI) from 0.005 to 3.15. Plaque prevalence was 25.9% to 90%; dental calculus from 4.7% to 59.41%; gingivitis from 36.7% to 100%. Bruxism was 10.3% to 73%, and dental trauma was 4.7% to 100%. This disparity in the results shows the heterogeneity of this population, with different degrees of socio-behavioral impairment, which can directly influence oral hygiene and the consequent accumulation of biofilm.

Construyo e validado de jogo educativo sobre sexualidade para adolescentes

Introdujo: a educagáo em saúde de adolescentes com foco na sexualidade é favorecida pelo emprego de tecnologias cuidativo- educacionais. Objetivo: construir e validar uma tecnologia educacional, do tipo jogo de tabuleiro, para mediar a discussáo de tópicos referentes a sexualidade com a populado adolescente. Materiais e métodos: estudo metodológico delineado em tres etapas, a saber: 1°) delimitado do conteúdo a ser abordado, 2°) elaborado do protótipo do jogo, 3°) validado do conteúdo e aparencia por juízes especialistas. Resultados e discussao: "Match" é um jogo de tabuleiro, com perguntas temáticas relacionados a sexualidade que direcionam o jogador do início ao fim do trajeto. Deu-se enfase a incorporado de elementos da gamificagáo, tais como, sorte, estratégia, competido e cooperado. O processo de validado obteve éxito em todas as categorias avaliadas, o jogo foi considerado relevante e adequado as atividades de educado em saúde. Conclusdes: O jogo elaborado encontra-se validado e disponível a comunidade científica e profissional, e deverá ter os seus efeitos mensurados nas próximas etapas da pesquisa. Além disso, uma versáo digital da ferramenta deve ser construída para elevar o seu alcance.

Introduction: health education for adolescents with a focus on sexuality is favored using care- educational technologies. Objective: to build and validate an educational technology, like a board game, to mediate the discussion of topics related to sexuality with the adolescent population. Materials and methods: methodological study outlined in three stages, namely: 1st) delimitation of the content to be addressed, 2nd) creation of the game prototype, 3rd) validation of the content and appearance by expert judges. Results and discussion: "Match" is a board game, with thematic questions related to sexuality that direct the player from the beginning to the end of the path. Emphasis was placed on the incorporation of gamification elements, such as luck, strategy, competition, and cooperation. The validation process was successful in all evaluated categories, the game was considered relevant and suitable for health education activities. Conclusions: The developed game is validated and available to the scientific and professional community, and its effects should be measured in the next stages of the research. In addition, a digital version of the tool must be built to increase its reach.

Introducción: la educación en salud de los adolescentes con enfoque en la sexualidad se ve favorecida por el uso de tecnologías cuidado - educativas. Objetivo: construir y validar una tecnología educativa, a modo de juego de mesa, para mediar en la discusión de temas relacionados a la sexualidad con la población adolescente. Materiales y métodos: estudio metodológico planteado en tres etapas, a saber: 1a) delimitación del contenido a abordar, 2a) creación del prototipo del juego, 3a) validación del contenido y apariencia por jueces expertos. Resultados y discusión: "Match" es un juego de mesa, con preguntas temáticas relacionadas con la sexualidad que dirigen al jugador desde el principio hasta el final del viaje. Se hizo hincapié en la incorporación de elementos de gamificación, como la suerte, la estrategia, la competencia y la cooperación. El proceso de validación fue exitoso en todas las categorías evaluadas, el juego fue considerado relevante y adecuado para actividades de educación en salud. Conclusiones: el juego desarrollado está validado y disponible para la comunidad científica y profesional, y sus efectos deben medirse en las próximas etapas de la investigación. Además, se debe construir una versión digital de la herramienta para aumentar su alcance.

Construção e validação de jogo educativo sobre sexualidade para adolescentes / Construction and validation of an educational game about sexuality for adolescents / Construcción y validación de un juego educativo sobre sexualidad para adolescentes

Introdução: a educação em saúde de adolescentes com foco na sexualidade é favorecida pelo emprego de tecnologias cuidativo-educacionais. Objetivo: construir e validar uma tecnologia educacional, do tipo jogo de tabuleiro, para mediar a discussão de tópicos referentes à sexualidade com a população adolescente. Materiais e métodos: estudo metodológico delineado em três etapas, a saber: 1º) delimitação do conteúdo a ser abordado, 2º) elaboração do protótipo do jogo, 3º) validação do conteúdo e aparência por juízes especialistas. Resultados e discussão: "Match" é um jogo de tabuleiro, com perguntas temáticas relacionados à sexualidade que direcionam o jogador do início ao fim do trajeto. Deu-se ênfase à incorporação de elementos da gamificação, tais como, sorte, estratégia, competição e cooperação. O processo de validação obteve êxito em todas as categorias avaliadas, o jogo foi considerado relevante e adequado às atividades de educação em saúde. Conclusões: O jogo elaborado encontra-se validado e disponível à comunidade científica e profissional, e deverá ter os seus efeitos mensurados nas próximas etapas da pesquisa. Além disso, uma versão digital da ferramenta deve ser construída para elevar o seu alcance.

Introduction: health education for adolescents with a focus on sexuality is favored using care-educational technologies. Objective: to build and validate an educational technology, like a board game, to mediate the discussion of topics related to sexuality with the adolescent population. Materials and methods: methodological study outlined in three stages, namely: 1st) delimitation of the content to be addressed, 2nd) creation of the game prototype, 3rd) validation of the content and appearance by expert judges. Results and discussion: "Match" is a board game, with thematic questions related to sexuality that direct the player from the beginning to the end of the path. Emphasis was placed on the incorporation of gamification elements, such as luck, strategy, competition, and cooperation. The validation process was successful in all evaluated categories, the game was considered relevant and suitable for health education activities. Conclusions: The developed game is validated and available to the scientific and professional community, and its effects should be measured in the next stages of the research. In addition, a digital version of the tool must be built to increase its reach.

Introducción: la educación en salud de los adolescentes con enfoque en la sexualidad se ve favorecida por el uso de tecnologías cuidado - educativas. Objetivo: construir y validar una tecnología educativa, a modo de juego de mesa, para mediar en la discusión de temas relacionados a la sexualidad con la población adolescente. Materiales y métodos: estudio metodológico planteado en tres etapas, a saber: 1ª) delimitación del contenido a abordar, 2ª) creación del prototipo del juego, 3ª) validación del contenido y apariencia por jueces expertos. Resultados y discusión: "Match" es un juego de mesa, con preguntas temáticas relacionadas con la sexualidad que dirigen al jugador desde el principio hasta el final del viaje. Se hizo hincapié en la incorporación de elementos de gamificación, como la suerte, la estrategia, la competencia y la cooperación. El proceso de validación fue exitoso en todas las categorías evaluadas, el juego fue considerado relevante y adecuado para actividades de educación en salud. Conclusiones: el juego desarrollado está validado y disponible para la comunidad científica y profesional, y sus efectos deben medirse en las próximas etapas de la investigación. Además, se debe construir una versión digital de la herramienta para aumentar su alcance.

Point-of-care ultrasound of peripheral nerves in the diagnosis of Hansen's disease neuropathy.

Introduction: Hansen's disease (HD) is the most common cause of treatable peripheral neuropathy in the world that may or may not involve skin manifestations, and physical examination based on simplified neurologic evaluation is a subjective and inaccurate procedure. High-resolution ultrasound (HRUS) can be used to evaluate peripheral nerves and is a validated technique of good reproducibility, permitting a detailed and precise examination. Objectives: We proposed to establish objective criteria for absolute values of the measurement of the CSA of peripheral nerves and their indices of the ΔCSA and ΔTpT in the diagnosis of Hansen's disease neuropathy as compared with healthy voluntaries. Materials and methods: In municipalities from different regions of Brazil, we randomly selected 234 volunteer Brazilian patients diagnosed with leprosy to be submitted to peripheral nerve echography and compared with 49 healthy Brazilian volunteers. Results: Hansen Disease assessed by high resolution ultrasound is a primarily neural disease that leads to multiple hypertrophic mononeuropathy characterized by CSA values exceeding normal limits (Med CT = 10.2 mm2; UT = 9.8 mm2; UPT = 9.3 mm2; CFFH = 18.3 mm2; T = 9.6 mm2), and the pattern of asymmetry (ΔCSA>2.5 mm2 with RR 13) and focality (ΔTPT > 2.5 mm2 with RR 6.4) of this thickening has higher sensitivity (76,1%) and specificity (87,8 %) for its early diagnosis that laboratory tests. Analyzing each subject, the percentage of thickened nerves detected among the total number of nerves assessed was higher among patients with HD than among healthy individuals (p < 0.0001). Individuals with two or more thickened nerves were at 24.1 times higher relative risk (95% CI: 6.74-88.98) of HD.

Evaluation of altered patterns of tactile sensation in the diagnosis and monitoring of leprosy using the Semmes-Weinstein monofilaments.

BACKGROUND: Leprosy neuropathy is the most common peripheral neuropathy of infectious etiology worldwide; it is characterized as asymmetric and focal multiple mononeuropathy. Semmes-Weinstein monofilament (SWM) test is a simple method to assess sensory nerve function. METHODS AND FINDINGS: In this prospective cohort study, a dermatologist carried out hands and feet tactile sensation test with SWM in 107 multibacillary leprosy patients at diagnosis and in 76 patients at the end of treatment from 2016 to 2019. At diagnosis, 81/107 (75.7%) patients had some degree of functional disability, and 46 (43%) of them had altered SWM-test in the hands and 94 (87.9%) of them in the feet. After one year of multibacillary multidrug therapy, the disability decreasing to 44/76 patients (57.9%) and decreasing of the percentual of patients with altered SWM-test to 18% for the hands, and to 28.7% for the feet. At the end of treatment, the number of SMW-test points presented improvement in the hands of 22 (28.9%) patients, and in the feet of 47 (61.8%). In the hands, by SWM-test, only the radial nerve point demonstrated a significant asymmetry, while in the feet, the difference between the sum of altered SWM-test points showed significant asymmetry between both sides, highlighting the tibial nerve for the establishment of asymmetric leprosy neuropathy. In Spearman's correlation analysis, a positive correlation with statistical significance was observed between the number of hands and feet SWM altered points at diagnosis and the degree of disability at diagnosis (0.69) and at the end of the treatment (0.80). CONCLUSION: The patterns of hands and feet tactile sensation at diagnosis and their consequent modifications with the anti-leprosy drugs define the bacterial etiology of neuropathy, an important tool for the clinical diagnosis and follow up of the disease, highlighting the tibial nerve findings, the most affected nerve among leprosy patients by SWM-test, with significant asymmetry and focality impairments.

Serious games para educação sexual de adolescentes e jovens: revisão integrativa de literatura / Serious games for sex education of adolescents and youth: integrative literature review

Resumo Objetiva-se mapear jogos educativos relacionados à sexualidade de adolescentes/jovens, a partir de publicações ocorridas na última década (2011 a 2020). Realizou-se uma revisão integrativa nas bases de dados Web of Science, SCOPUS, MEDLINE/PubMed e LILACS em setembro de 2020, com a inclusão final de 16 estudos. Identificaram-se jogos desenvolvidos em quatro continentes, com predominância em países de renda alta, sendo os Estados Unidos e o Brasil os que mais publicaram. As ferramentas eram, em sua maioria, videogames e jogos digitais, com a adoção de cenários e enredos simulados. Sobre o desenvolvimento, houve preferência pelo modo de construção participativo; alguns também passaram por processo de validação. Abordavam diversas áreas da sexualidade humana e demonstravam potencial positivo para uso, com ganhos educacionais e mudança de hábitos, mas seus resultados precisam ser ponderados. Apenas três jogos tiveram seus efeitos avaliados por estudos controlados e randomizados.

Abstract This study aims to map educational games related to the sexuality of adolescents/young people, based on publications that occurred over the last decade (2011 to 2020). An integrative review was performed in the Web of Science, SCOPUS, MEDLINE/PubMed, and LILACS databases in September 2020, with the final inclusion of 16 studies. Games developed on four continents were identified, predominantly in high-income countries, with the United States of America and Brazil being those that published the most. The tools consisted mostly of videogames and digital games, with the adoption of simulated scenarios and plots. Regarding development, there was a preference for the participatory construction mode, while some also underwent a validation process. The tools addressed several areas of human sexuality and demonstrated a positive potential for use, with educational gains and changes in habits, but their results need to be carefully considered. Only the effects of three games were evaluated by randomized controlled trials.

Serious games for sex education of adolescents and youth: integrative literature review. / Serious games para educação sexual de adolescentes e jovens: revisão integrativa de literatura.

This study aims to map educational games related to the sexuality of adolescents/young people, based on publications that occurred over the last decade (2011 to 2020). An integrative review was performed in the Web of Science, SCOPUS, MEDLINE/PubMed, and LILACS databases in September 2020, with the final inclusion of 16 studies. Games developed on four continents were identified, predominantly in high-income countries, with the United States of America and Brazil being those that published the most. The tools consisted mostly of videogames and digital games, with the adoption of simulated scenarios and plots. Regarding development, there was a preference for the participatory construction mode, while some also underwent a validation process. The tools addressed several areas of human sexuality and demonstrated a positive potential for use, with educational gains and changes in habits, but their results need to be carefully considered. Only the effects of three games were evaluated by randomized controlled trials.

Objetiva-se mapear jogos educativos relacionados à sexualidade de adolescentes/jovens, a partir de publicações ocorridas na última década (2011 a 2020). Realizou-se uma revisão integrativa nas bases de dados Web of Science, SCOPUS, MEDLINE/PubMed e LILACS em setembro de 2020, com a inclusão final de 16 estudos. Identificaram-se jogos desenvolvidos em quatro continentes, com predominância em países de renda alta, sendo os Estados Unidos e o Brasil os que mais publicaram. As ferramentas eram, em sua maioria, videogames e jogos digitais, com a adoção de cenários e enredos simulados. Sobre o desenvolvimento, houve preferência pelo modo de construção participativo; alguns também passaram por processo de validação. Abordavam diversas áreas da sexualidade humana e demonstravam potencial positivo para uso, com ganhos educacionais e mudança de hábitos, mas seus resultados precisam ser ponderados. Apenas três jogos tiveram seus efeitos avaliados por estudos controlados e randomizados.

Development, validation, and application of a Brazilian sleep myths and truths assessment scale (SLEEP-MTAS).

OBJECTIVE: This study aimed to develop, validate, and apply a scale assessing knowledge of sleep-related myths and truths and associate it with sociodemographic factors. METHODS: A scale with 15 questions was created, containing statements about the characteristics of sleep and related to sleep and dentistry. Each answer ranged from 0 to 4 points, generating a total score from 0 to 60, where higher scores represented greater knowledge. A preliminary study with 200 people assessed its convergent and discriminant construct validity, internal consistency, and temporal stability. The main study included 1965 respondents over 18 years. Additionally, sociodemographic data were collected and a classification of the level of knowledge was performed. Data were analyzed with Student's t-test and one-way ANOVA (p < 0.05). RESULTS: The questionnaire showed convergent (p < 0.001) and discriminant (p = 0.024) construct validity, internal consistency (alpha = 0.7), and temporal stability (ICC = 0.87). In the main study, 90.3% of the participants had moderate and high knowledge, with the score ranging from 24 to 58. Adults over 28 years old (p < 0.001), from the southern region of Brazil (p < 0.001), who lived in capital or metropolitan areas (p < 0.001), with higher education (p < 0.001), without religion (p < 0.001), and involved in dentistry (p < 0.001) had greater knowledge than their peers. CONCLUSIONS: The scale presented good psychometric properties. Most participants had moderate and high knowledge on sleep, with a difference in knowledge related to the age, region and area of residence, education, involvement with dentistry, and religion.

Silent peripheral neuropathy determined by high-resolution ultrasound among contacts of patients with Hansen's disease.

Introduction: Hansen's disease (HD) primarily infects peripheral nerves, with patients without HD being free of peripheral nerve damage. Household contacts (HHCs) of patients with HD are at a 5-10 times higher risk of HD than the general population. Neural thickening is one of the three cardinal signs that define a case of HD according to WHO guidelines, exclusively considering palpation examination that is subjective and may not detect the condition in the earliest cases even when performed by well-trained professionals. High-resolution ultrasound (HRUS) can evaluate most peripheral nerves, a validated technique with good reproducibility allowing detailed and accurate examination. Objective: This study aimed to use the peripheral nerve HRUS test according to the HD protocol as a diagnostic method for neuropathy comparing HHCs with healthy volunteers (HVs) and patients with HD. Methods: In municipalities from 14 different areas of Brazil we selected at random 83 HHC of MB-patients to be submitted to peripheral nerve ultrasound and compared to 49 HVs and 176 HD-patients. Results: Household contacts assessed by HRUS showed higher median and mean absolute peripheral nerve cross-sectional area (CSA) values and greater asymmetries (ΔCSA) compared to HVs at the same points. Median and mean absolute peripheral nerve CSA values were higher in patients with HD compared to HCCs at almost all points, while ΔCSA values were equal at all points. Mean ± SD focality (ΔTpT) values for HHCs and patients with HD, respectively, were 2.7 ± 2.2/2.6 ± 2.2 for the median nerve, 2.9 ± 2.7/3.3 ± 2.9 for the common fibular nerve (p > 0.05), and 1.3 ± 1.3/2.2 ± 3.9 for the ulnar nerve (p < 0.0001). Discussion: Considering HRUS findings for HHCs, asymmetric multiple mononeuropathy signs (thickening or asymmetry) in at least 20% of the nerves evaluated could already indicates evidence of HD neuropathy. Thus, if more nerve points are assessed in HHCs (14 instead of 10), the contacts become more like patients with HD according to nerve thickening determined by HRUS, which should be a cutting-edge tool for an early diagnosis of leprosy cases.

Processo de trabalho na vigilância em saúde no Brasil: uma scoping review / Work process in health surveillance in Brazil: a scoping review

Resumo Introdução A descentralização da vigilância em saúde demandou dos municípios uma reorganização dos processos de trabalho. Ao passo que aproximou o serviço do território, também suscitou desafios em formular propostas de intervenção sobre problemas de saúde. Objetivo Analisar o processo de trabalho dos profissionais que executam ações de vigilância em saúde no Brasil. Método Estudo de revisão da literatura de tipo scoping review. A estratégia de busca incluiu a consulta em bases de dados, Google Scholar e listas de referências, para artigos publicados entre 2000 e 2016. As buscas foram realizadas em abril de 2017 por dois pesquisadores, separadamente. A amostra compõe-se de 38 estudos. Resultados Resultaram cinco categorias: descentralização das ações de vigilância; vigilância em saúde e o trabalho em rede; processos educativos; análise territorial, e participação social. O processo descentralizado aproximou a vigilância do território e da comunidade, favorecendo a sua capacidade antecipatória, o trabalho intersetorial e a atuação ajustada aos problemas. Contudo, a descentralização ainda é um desafio na construção da vigilância como um trabalho em rede que tenha, como ponto de partida, a atenção básica, através de uma análise territorial. A resposta ao desafio exige processos de educação permanente e o engajamento participativo da comunidade e do controle social. Conclusão A descentralização exige ações conjuntas dos trabalhadores da vigilância e da atenção, tendo como base o território e as ações de cunho intersetorial e com participação social.

Abstract Introduction Decentralization of health surveillance required municipalities to reorganize work processes. While bringing the service closer to the territory, it also raised challenges in formulating intervention proposals on health problems. Objective to analyze the work process of professionals who perform health surveillance actions in Brazil. Method This is a literature scoping review study. The search strategy included health databases, Google Scholar, and reference lists for articles published between 2000 and 2016. Searches were conducted in April 2017 by two researchers separately. The sample consists of 38 studies. Results Five categories emerged: decentralization of surveillance actions, health surveillance and networking, educational processes, territorial analysis, and social participation. The decentralized process approached the surveillance of the territory and the community, favoring its anticipatory capacity, the intersectoral work, and the action adjusted to the problems. However, this approach is still a challenge for the construction of the surveillance as a network, based on the primary care through a territorial analysis. The answer to this challenge demands processes of permanent education and the participative compromise of the community and social control. Conclusion The decentralization requires united actions of the professionals of surveillance and attendance, having as focus the territory, intersectoral actions, and social participation.

No Evidence of SARS-CoV-2 Infection in Neotropical Primates Sampled During COVID-19 Pandemic in Minas Gerais and Rio Grande do Sul, Brazil.

In 2019, a new coronavirus disease (COVID-19) was detected in China. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was capable to infect domestic and captive mammals like cats, tigers and minks. Due to genetic similarities, concern about the infection of non-human primates (NHPs) and the establishment of a sylvatic cycle has grown in the Americas. In this study, neotropical primates (NP) were sampled in different areas from Brazil to investigate whether they were infected by SARS-CoV-2. A total of 89 samples from 51 NP of four species were examined. No positive samples were detected via RT-qPCR, regardless of the NHP species, tissue or habitat tested. This work provides the first report on the lack of evidence of the circulation of SARS-CoV-2 in NP. The expansion of wild animals sampling is necessary to understand their role in the epidemiology of SARS-CoV-2 and other potentially zoonotic pathogens in natural environments shared by humans.

Innovative tracking, active search and follow-up strategies for new leprosy cases in the female prison population.

BACKGROUND: Regarding the leprosy transmission through the upper airways, overcrowded locations such as prisons can become a risk to get sick. Like the leprosy hidden endemic demonstrated in male prison population, being interesting to assess the leprosy scene also among confined women. METHODS: A prospective descriptive study conducted at Female Penitentiary, Brazil. Leprosy Suspicion Questionnaire (LSQ) were applied to the participants, and submitted to specialized dermatoneurological exam, peripheral nerve ultrasonography, and anti-PGL-I serology. FINDINGS: 404 female inmates were evaluated, 14 new cases were diagnosed (LG-leprosy group), a new case detection rate (NCDR) of 3.4%, 13 multibacillary, while another 390 constituted the Non-Leprosy group (NLG). Leprosy cases were followed up during multidrug therapy with clinical improvement. The confinement time median was 31 months in LG, similar to NLG, less than the time of leprosy incubation. Regarding LSQ, the neurological symptoms reached the highest x2 values as Q1-numbness (5.6), Q3-anesthetizes areas in the skin (7.5), Q5-Stinging sensation (5.8), and Q7-pain in the nerves (34.7), while Q4-spots on the skin was 4.94. When more than one question were marked in the LSQ means a 12.8-fold higher to have the disease than a subject who marked only one or none. The high 34% rate of anti-PGL-I seropositivity in the penitentiary, higher levels in LG than NLG. Three additional leprosy cases each were diagnosed on the second (n = 66) and third (n = 14) reevaluations 18 and 36 months after the initial one. Semmes-Weinstein monofilaments demonstrated lower limbs (32.2%) more affected than the upper limbs (25%) with improvement during the follow-up. INTERPRETATION: The NCDR in this population showed an hidden endemic of leprosy as well as the efficacy of a search action on the part of a specialized team with the aid of the LSQ and anti-PGL-I serology as an auxiliary tracking tools.

Active search strategies, clinicoimmunobiological determinants and training for implementation research confirm hidden endemic leprosy in inner São Paulo, Brazil.

BACKGROUND: This study evaluates implementation strategies for leprosy diagnosis based on responses to a Leprosy Suspicion Questionnaire (LSQ), and analyzes immunoepidemiological aspects and follow-up of individuals living in a presumptively nonendemic area in Brazil. METHODOLOGY/PRINCIPAL FINDINGS: Quasi-experimental study based on LSQ throughout Jardinópolis town by community health agents, theoretical-practical trainings for primary care teams, dermatoneurological examination, anti-PGL-I serology, RLEP-PCR, and spatial epidemiology. A Leprosy Group (LG, n = 64) and Non-Leprosy Group (NLG, n = 415) were established. Overall, 3,241 LSQs were distributed; 1,054 (32.5%) LSQ were positive for signs/symptoms (LSQ+). Among LSQ+ respondents, Q2-Tingling (pricking)? (11.8%); Q4-Spots on the skin? (11.7%); Q7-Pain in the nerves? (11.6%); Q1-Numbness in your hands and/or feet? (10.7%) and Q8-Swelling of hands and feet? (8.5%) were most frequently reported symptoms. We evaluated 479 (14.8%) individuals and diagnosed 64 new cases, a general new case detection rate (NCDR) of 13.4%; 60 were among 300 LSQ+ (NCDR-20%), while 4 were among 179 LSQ negative (NCDR-2.23%). In LG, Q7(65%), Q2(60%), Q1(45%), Q4(40%) and Q8(25%) were most frequent. All 2x2 crossings of these 5 questions showed a relative risk for leprosy ranging from 3 to 5.8 compared with NLG. All patients were multibacillary and presented hypochromatic macules with loss of sensation. LG anti-PGL-I titers were higher than NLG, while 8.9% were positive for RLEP-PCR. The leprosy cases and anti-PGL-I spatial mappings demonstrated the disease spread across the town. CONCLUSIONS/SIGNIFICANCE: Implementation actions, primarily LSQ administration focused on neurological symptoms, indicate hidden endemic leprosy in a nonendemic Brazilian state.

Agaricus bisporus mushroom as partial fat replacer improves the sensory quality maintaining the instrumental characteristics of beef burger.

The effect of Agaricus bisporus (AB) mushroom as a fat replacer in beef burgers was evaluated. Four treatments were compared: control (0% AB, 20% fat) and 3 treatments with partial fat replacement: AB 5% (5% AB, 15% fat); AB 10% (10% AB, 10% fat); AB 15% (AB 15%, 5% fat). Mushroom addition increased moisture content and yield and decreased cooking loss and diameter reduction of the burgers. Formulations with the highest AB concentrations were lighter and redder than the control. AB incorporation affected texture, resulting in less hard and less chewy products. In general, lipid oxidation increased during the storage time, however the lowest values were observed in AB 15%, i.e. the lowest fat content and the presence of AB increased the oxidative stability. AB 10% and AB 15% had good overall liking and were sensorially characterized as juicy, tender and flavorsome burgers. The partial replacement of animal fat by AB is a promising strategy for developing a low-fat burger.

Análise institucional da vigilância em saúde em um município da região metropolitana de Porto Alegre/RS / Institutional analysis of health surveillance in a city in the Metropolitan Region of Porto Alegre, Rio Grande do Sul, Brazil

Resumo O artigo tem por objetivo identificar e analisar a institucionalização das ações de vigilância em saúde em um município da região metropolitana de Porto Alegre/RS visando perceber suas dimensões instituídas e instituintes, bem como caracterizar as lógicas de atuação dessa vigilância. O referencial teórico-metodológico utilizado foi a análise institucional, na vertente socioanalítica. A pesquisa foi realizada em dois momentos, usando como ferramentas de coleta de informações a observação participante, as reuniões de grupo com o coletivo dos trabalhadores e o diário da primeira autora. Apareceram como resultados a institucionalização da vigilância - em decorrência do analisador "identificação de toda vigilância com a vigilância sanitária" -, a relevância do trabalho em rede - a partir de situações pontuais - e a fragilidade da vigilância da saúde do trabalhador, tendo presente o analisador da invisibilidade dessa vigilância no sistema. Foram observadas ações educativas - especialmente na vigilância sanitária -, processos instituintes e parcerias entre a vigilância em saúde e a atenção básica e entre as diferentes vigilâncias. Por fim, percebeu-se que nas ações intersetoriais é importante ter presentes as diferenças culturais, históricas e funcionais dos diferentes setores envolvidos.

Abstract The article aims to identify and analyze the institutionalization of Health Surveillance actions in a municipality in the metropolitan region of Porto Alegre, Rio Grande do Sul, Brazil, aiming to perceive its instituted and instituting dimensions, as well as to characterize the logic of this Surveillance's performance. The theoretical-methodological framework used was Institutional Analysis, in the socio-analytical aspect. The research was carried out in two moments, using participant observation, group meetings with the workers' collective and the diary of the first author as a tool for collecting information. As a result, the following emerged: the institutionalization of surveillance - due to the analyzer "identification of all surveillance with health surveillance"; the relevance of networking - from specific situations; and the fragility of the worker health surveillance, bearing in mind the analyzer for the invisibility of this surveillance in the system. Educational actions were observed - especially at health surveillance - instituting processes; partnership between health surveillance and primary healthcare and between the different surveillances. Finally, it was realized that in intersectoral actions it is important to bear in mind the cultural, historical and functional differences of the different sectors involved.

Leprosy in a prison population: A new active search strategy and a prospective clinical analysis.

BACKGROUND: This study evaluates an active search strategy for leprosy diagnosis based on responses to a Leprosy Suspicion Questionnaire (LSQ), and analyzing the clinical, immunoepidemiological and follow-up aspects for individuals living in a prison population. METHODS: A cross-sectional study based on a questionnaire posing 14 questions about leprosy symptoms and signs that was distributed to 1,400 prisoners. This was followed by dermatoneurological examination, anti-PGL-I serology and RLEP-PCR. Those without leprosy were placed in the Non-leprosy Group (NLG, n = 1,216) and those diagnosed with clinical symptoms of leprosy were placed in the Leprosy Group (LG, n = 34). FINDINGS: In total, 896 LSQ were returned (64%), and 187 (20.9%) of the responses were deemed as positive for signs/symptoms, answering 2.7 questions on average. Clinically, 1,250 (89.3%) of the prisoners were evaluated resulting in the diagnosis of 34 new cases (LG), based on well-accepted clinical signs and symptoms, a new case detection rate of 2.7% within this population, while the NLG were comprised of 1,216 individuals. The confinement time medians were 39 months in the LG while it was 36 months in the NLG (p>0.05). The 31 leprosy cases who responded to the questionnaire (LSQ+) had an average of 1.5 responses. The symptoms "anesthetized skin area" and "pain in nerves" were most commonly mentioned in the LG while "tingling, numbness in the hands/feet", "sensation of pricks and needles", "pain in nerves" and "spots on the skin" responses were found in more than 30% of questionnaires in the NLG. Clinically, 88.2% had dysesthetic macular skin lesions and 97.1% presented some peripheral nerve impairment, 71.9% with some degree of disability. All cases were multibacillary, confirming a late diagnosis. Anti-PGL-I results in the LG were higher than in the NLG (p<0.0001), while the RLEP-PCR was positive in 11.8% of the patients. INTERPRETATION: Our findings within the penitentiary demonstrated a hidden prevalence of leprosy, although the individuals diagnosed were likely infected while living in their former communities and not as a result of exposure in the prison. The LSQ proved to be an important screening tool to help identify leprosy cases in prisons.

Novel frameshift variant of the CFTR gene: S511Lfs*2 from phenotype to molecular predictions.

Cystic fibrosis (CF) is a genetic disease caused by variants in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. There are over 2,000 different pathogenic and non-pathogenic variants described in association with a broad clinical heterogeneity. In this work, we identified a novel variant S511Lfs*2 in CFTR gene that has not been reported in patients with CF. The patient was a female genotyped with c.1000C>T (legacy name: R334W) variant (pathogenic, CF-causing) and the novel variant (S511Lfs*2). We verified the amino acid sequence, the protein structure, and predicted the pathogenicity employing computational analysis. Our findings showed that S511Lfs*2 is a frameshift variant and suggest that it is associated with severe CF phenotype, as it leads to a lack of CFTR protein synthesis, and consequently the loss of its functional activity.

Is there association between dental malocclusion and bruxism? A systematic review and meta-analysis.

OBJECTIVES: Given that current literature largely dissociates dental malocclusion and bruxism, the objective of this study was to gather, through a systematic review, scientific evidence to support their relationship. METHODOLOGY: This study was performed according to the PECO strategy (where P = general population; E = dental malocclusion; C = no dental malocclusion; and O = bruxism). Literature searches were conducted without language or date restrictions in the following databases: PubMed, Scopus, the Web of Science, the Cochrane Library, LILACS/BBO via VHL and the grey literature. The search strategy included Medical Subject Headings/DECs, synonyms and free terms relevant to each database, with no age restrictions applied. Once the relevant data were extracted from the articles, the Fowkes and Fulton guidelines were followed to assess the quality and risk of bias. For quantitative analysis, dental malocclusions were divided into groups according to their type in order to perform odds ratio (OR) meta-analyses with 95% confidence intervals (CI) using the Review Manager software program (Cochrane, London, UK). The level of certainty of evidence was demonstrated through the Grading of Recommendations Assessment, Development, and Evaluation (GRADE). RESULTS: After 1,502 studies found, 10 studies were included for qualitative analysis and nine for quantitative synthesis. Four studies presented high methodological quality. Five meta-analyses suggested a non-association between bruxism and Angle class I (OR: 1.05, 95% CI: 0.41-2.69; P = .92; I2  = 84%), Angle class II (OR: 1.49, 95% CI: 0.77-2.87; P = .23; I2  = 71%) or Angle class III (OR: 0.77, 95% CI: 0.31-1.93; P = .58; I2  = 0%). Bruxism was associated with children who did not present with a posterior crossbite (OR: 0.70, 95% CI: 0.51-0.96; P = .03; I2  = 27%) and present crowding (OR: 1.53, 95% CI: 1.03-2.26; P = .03; I2  = 0%). The GRADE analysis presented a very low quality of evidence. CONCLUSION: Individuals who present with bruxism have a greater chance of crowding. However, bruxism is not associated with the presence of any of the other malocclusions evaluated.

Is There Association Between Stress and Bruxism? A Systematic Review and Meta-Analysis.

This systematic review and meta-analysis aimed to investigate a possible association between stress and bruxism in humans. This study was conducted according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines under the code CRD42020188862, and the searches were performed on the following databases: PubMed, Scopus, Web of Science, Cochrane, LILACS, OpenGrey, and Google Scholar. This systematic review evaluated observational studies in adult humans with and without stress to verify the association between bruxism and the presence of stress. The risk of bias was evaluated through the Joanna Briggs Institute Critical Appraisal Tools for Analytical Cross-Sectional Studies. In quantitative analysis, the Odds Ratio (OR) and their 95% confidence interval (CI) were calculated through a fixed-effect model. Furthermore, a summary of the overall strength of evidence was presented using the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE). A total of 1,458 studies were identified, and six were included in this systematic review. Two studies included were classified with a low risk of bias, and the others were classified with a moderate risk of bias. In three articles, a meta-analysis was performed and showed an association between these two factors (OR 2.07 [1.51, 2.83], p < 0.00001, I 2 = 45%). Besides that, a low certainty of the evidence was detected among this association. Stressed individuals show a higher chance of presenting bruxism when compared to healthy individuals. Despite the low heterogeneity found in the quantitative analysis among the articles reporting an association between stress and bruxism, further studies with similar methods are necessary to understand this relationship better.

A Low-Cost and Simple Genetic Screening for Cystic Fibrosis Provided by the Brazilian Public Health System.

Cystic fibrosis newborn screening was implemented in Brazil by the Public Health System in 2012. Because of cost, only 1 mutation was tested - p.Phe508del. We developed a robust low-cost genetic test for screening 11 CFTR gene mutations with potential use in developing countries.