Contextual situations in competitions that influence well-being and recovery of Futsal athletes before home official matches

Introduction: contextual variables associated with competitive stress may affect the perception of the well-being and recovery of futsal athletes.Material and Methods : twenty male professional futsal players responded to the Hooper Index (HI) and Total Quality of Recovery Scale (TQR) two hours before eleven official matches. Data were collected on age, predicted game difficulty, distance from the previous game, time interval since the previous game, ranking of the team and opponent, and outcome of the previous game of the team and the opponent (defeat/draw/win). Multivariate logistic regression analysis and the Spearman rank-sum test were used to identify stressors that influenced HI and TQR scores, considering p<0.05.Resultsthe HI was higher in the National League (11.2 ± 2.9 a.u., p<0.005) compared to the State championship (10.0 ± 2.4 a.u.). The DOMS were higher in National League (p<0.001) and games preceded by victory (p<0.005). The HI (r=-0.53, p<0.001), age (r=-0.18, p<0.01), and muscle pain (r = -0.39, p <0.001) correlated with the TQR. The TQR was higher in games preceded by defeat (15.5 ± 1.6) compared to victory (14.6 ± 1.7, p<0.01). The pre-game HI and TQR scores were not significantly different (p>0.05) in games that ended in victory, draw or defeat.Conclusionthe results suggest that the DOMS scores of HI and TQR reported before at home official Futsal games are correlated with contextual factors including the level of championship and outcome of the last game. (AU)

Plasmatic higher levels of homocysteine in non-alcoholic fatty liver disease (NAFLD).

BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease, which includes a spectrum of hepatic pathology such as simple steatosis, steatohepatitis, fibrosis and cirrhosis. The increased serum levels of homocysteine (Hcy) may be associated with hepatic fat accumulation. Genetic mutations in the folate route may only mildly impair Hcy metabolism. The aim of this study was to investigate the relation between liver steatosis with plasma homocysteine level and MTHFR C677T and A1298C polymorphisms in Brazilian patients with NAFLD. METHODS: Thirty-five patients diagnosed with NAFLD by liver biopsy and forty-five healthy controls neither age nor sex matched were genotyped for C677T and A1298C MTHFR polymorphisms using PCR-RFLP and PCR-ASA, respectively, and Hcy was determined by HPLC. All patients were negative for markers of Wilson's, hemochromatosis and autoimmune diseases. Their daily alcohol intake was less than 100 g/week. A set of metabolic and serum lipid markers were also measured at the time of liver biopsies. RESULTS: The plasma Hcy level was higher in NAFLD patients compared to the control group (p = 0.0341). No statistical difference for genotypes 677C/T (p = 0.110) and 1298A/C (p = 0.343) in patients with NAFLD and control subjects was observed. The genotypes distribution was in Hardy-Weinberg equilibrium (677C/T p = 0.694 and 1298 A/C p = 0.188). The group of patients and controls showed a statistically significant difference (p < 0.001) for BMI and HOMA_IR, similarly to HDL cholesterol levels (p < 0,006), AST, ALT, γGT, AP and triglycerides levels (p < 0.001). A negative correlation was observed between levels of vitamin B12 and Hcy concentration (p = 0.005). CONCLUSION: Our results indicate that plasma Hcy was higher in NAFLD than controls. The MTHFR C677T and A1298C polymorphisms did not differ significantly between groups, despite the 677TT homozygous frequency was higher in patients (17.14%) than in controls (677TT = 4.44%) (p > 0.05). The suggested genetic susceptibility to the MTHFR C677T and A1298C should be confirmed in large population based studies.

Polymorphisms involved in folate metabolism pathways and the risk of the development of childhood acute leukemia.

OBJECTIVE: Polymorphisms that reduce the activity of reduced folate carrier (RFC) and methylenetetrahydrofolate reductase (MTHFR) and double (2R2R) or triple (3R3R) 28-bp tandem repeats in the promoter region of thymidylate synthase (TS) have been associated with the risk of childhood acute leukemia (AL). A case-control genotyping study was conducted in Brazilian children with the aim of investigating RFC, MTHFR, and TS polymorphisms as risk factors. METHODS: The polymerase chain reaction-restriction fragment length polymorphism method was employed in 177 AL cases and 390 controls. RESULTS: The presence of the mutant 1298C, also RFC 80A, was linked to a decreased risk of developing acute lymphoid leukemia (ALL) (odds ratio (OR)=0.46, 95% confidence interval (CI)=0.30-071 and OR=0.51, 95% CI=0.28-0.0.93, respectively). CONCLUSIONS: The genotype 677 CT was associated with increased risk of developing ALL (OR=1.6, 95% CI=1.1-2.7). Further epidemiological study is needed to unravel the role of complex multiple gene-environment interactions in leukemogenesis.