Validation of Serological Methods for COVID-19 and Retrospective Screening of Health Employees and Visitors to the São Paulo University Hospital, Brazil.

Reliable serological tests for the detection of SARS-CoV-2 antibodies among infected or vaccinated individuals are important for epidemiological and clinical studies. Low-cost approaches easily adaptable to high throughput screenings, such as Enzyme-Linked Immunosorbent Assays (ELISA) or electrochemiluminescence immunoassay (ECLIA), can be readily validated using different SARS-CoV-2 antigens. A total of 1,119 serum samples collected between March and July of 2020 from health employees and visitors to the University Hospital at the University of São Paulo were screened with the Elecsys® Anti-SARS-CoV-2 immunoassay (Elecsys) (Roche Diagnostics) and three in-house ELISAs that are based on different antigens: the Nucleoprotein (N-ELISA), the Receptor Binding Domain (RBD-ELISA), and a portion of the S1 protein (ΔS1-ELISA). Virus neutralization test (CPE-VNT) was used as the gold standard to validate the serological assays. We observed high sensitivity and specificity values with the Elecsys (96.92% and 98.78%, respectively) and N-ELISA (93.94% and 94.40%, respectively), compared with RBD-ELISA (90.91% sensitivity and 88.80% specificity) and the ΔS1-ELISA (77.27% sensitivity and 76% specificity). The Elecsys® proved to be a reliable SARS-CoV-2 serological test. Similarly, the recombinant SARS-CoV-2 N protein displayed good performance in the ELISA tests. The availability of reliable diagnostic tests is critical for the precise determination of infection rates, particularly in countries with high SARS-CoV-2 infection rates, such as Brazil. Collectively, our results indicate that the development and validation of new serological tests based on recombinant proteins may provide new alternatives for the SARS-CoV-2 diagnostic market.

O preparo para o autocuidado do cliente diabético e família / The nursing care oriented to conduct to the realization of the care by the client and relatives

Relato parcial, quanti-qualitativo, de pesquisa-açäo realizada em unidade de internaçäo piloto/ambulatório do Hospital de Ensino, extensivo a moradia. Objetivo: estabelecer modelos de integraçäo hospital/comunidade, visando o indivíduo na unidade familiar, face à recuperaçäo/reabilitaçäo e integraçäo ao trabalho/sociedade, considerando os problemas de moradia/trabalho e aplicando a proposiçäo do SUDS na extensäo das açöes hospitalares a comunidade. Visitas domiciliares levantam as condiçöes da moradia, consolidando a participaçäo familiar no processo. Durante a internaçäo os cuidados de enfermagem realizados säo orientados, conduzindo à participaçäo e à realizaçäo destes, pelo cliente e familiares.