Detection of domoic acid in northern anchovies and California sea lions associated with an unusual mortality event.

The occurrence of an unusual mortality event involving California sea lions (Zalophus californianus) along the central California coast in May 1998 was recently reported. The potent neurotoxin domoic acid (DA), produced naturally by the diatom Pseudo-nitzschia australis and transmitted to the sea lions via planktivorous northern anchovies (Engraulis mordax), was identified as the probable causative agent. Details of DA analyses for anchovy tissues and sea lion feces are described. Domoic acid levels were estimated in anchovy samples by HPLC-UV, and in sea lion feces using the same method as well as a microplate receptor binding assay, with absolute confirmation by tandem mass spectrometry. The highest DA concentrations in anchovies occurred in the viscera (223 +/- 5 microg DA g(-1)), exceeding values in the body tissues by seven-fold and suggesting minimal bioaccumulation of DA in anchovy tissue. HPLC values for DA in sea lion fecal material (ranging from 152 to 136.5 microg DA g(-1)) required correction for interference from an unidentified compound. Inter-laboratory comparisons of HPLC data showed close quantitative agreement. Fecal DA activity determined using the receptor binding assay corresponded with HPLC values to within a factor of two. Finally, our detection of P. australis frustules, via scanning electron microscopy, in both anchovy viscera and fecal material from sea lions exhibiting seizures provides corroborating evidence that this toxic algal species was involved in this unusual sea lion mortality event.

A survey of larval and adult mosquitoes on the flood plains of Bangladesh, in relation to flood-control activities.

A mosquito survey was carried out between October 1991 and November 1992 on the flood plains of Bangladesh, as part of a baseline study designed to help predict the effects on vector-borne diseases of embanking rivers under the Bangladesh Government's Flood Action Plan. Overall, 15 species of larval and 15 species of adult anophelines were collected, along with 13 larval and 21 adult culicines. Anophelines made up only 6% of the mosquitoes caught at human bait. The most abundant anopheline biting man was Anopheles vagus, the dominant species in all nine sampling villages. The other recognized malaria vectors in the flood plains of Bangladesh, namely An. philippinensis, An. aconitus and An. annularis, were collected but in relatively low numbers. Culex vishnui was the most abundant biting culicine in all villages. Biting mosquitoes showed a bimodal seasonality of biting, with peaks between February and April and September and October. All biting mosquitoes showed a significant preference for outdoor biting. The densities of the mosquito populations are more likely to be affected by the large-scale environmental changes which have occurred in Bangladesh, as well as the dramatic increase in the density of the human population, than by the river embankments per se.

Differential lipid reserves influence host-seeking behaviour in the mosquitoes Aedes cantans and Aedes punctor.

Lipid reserves of bait-caught female Ae.cantans and Ae.punctor mosquitoes (Diptera: Culicidae) were significantly higher than in teneral females. Female Ae.cantans given access to 10% w/v sucrose solution post-emergence showed an ability to synthesize lipid and, after 192 h, they were willing to take a bloodmeal from a human volunteer. At this point, mean lipid reserves were not significantly different from mean lipid reserves of bait-caught females. Prior to 192h, females would not take a bloodmeal and lipid reserves were significantly lower than in bait-caught females. Female Ae.punctor given access to 10% w/v sucrose solution post-emergence also showed an ability to synthesize lipid. Females of this species were willing to feed from a human host after only 48 h, at which point lipid content was not significantly different from that in bait-caught females. The level of lipid reserves in females coming to bait differs significantly between species: Ae.cantans has lipid reserves approximately double those of Ae.punctor. In addition, Ae.punctor is able to synthesize lipid to a level comparable with that found in bait-caught females after only 24 h, whilst it takes 192 h for Ae.cantans females to synthesize the amount of lipid found in host-seeking females, when allowed free access to sugar. Physiological differences in lipid synthesis and the level of lipid reserves required may therefore explain the differences observed between the species in the time taken to initiate host seeking.