RESUMO
The title structure, C14H19ClO5, consists of an allene substituted with a Cl atom at one end joined at the other end to a D-galactopyranose ring bearing two fused isopropylidene groups.
Assuntos
Galactose/análogos & derivados , Cristalografia por Raios X , Galactose/química , Conformação Molecular , Estrutura MolecularRESUMO
Two unrelated molecules of the title compound, ethyl 1-(5-deoxy-1,2-O-isopropylidene-alpha-D-xylofuranos-5-C- yl)-1,2,3-triazole-5-carboxylate, C13H19N3O6, that are not linked by hydrogen bonding, comprise the asymmetric unit. There are no unusual bond lengths or angles. The two molecules differ in the degree of rotation around the methylene C atom that joins the triazole ring to the sugar part of the molecule. Molecules of the same conformation form infinite chains joined by hydrogen bonding between a H atom on the hydroxyl group of one molecule and an N atom in the triazole ring of another molecule generated by the 2(1) screw axis. Relevant intermolecular N...O distances are 3.013 (3) and 2.806 (3) A.
Assuntos
Xilose/análogos & derivados , Cristalização , Ligação de Hidrogênio , Xilose/químicaRESUMO
Reported herein is the X-ray crystallographic structure of a novel 10-oxygenated secotrinervitane diterpene, 3 alpha, 10 alpha-diacetoxy-7,16-secotrinervita-7,11,15(17)-triene (4), from soldiers of the endemic Madagascan termite Nasutitermes canaliculatus, which was compared with an energy-minimized structure obtained by computer molecular modeling. We also report 1H- and 13C-NMR and MS data for this new diterpene.
Assuntos
Diterpenos/isolamento & purificação , Insetos/química , Animais , Diterpenos/química , Espectroscopia de Ressonância Magnética , Masculino , Espectrometria de Massas , Modelos Estruturais , Conformação Molecular , Difração de Raios XRESUMO
Previously, (R)-quinuclidinyl (R)-4-iodobenzilate ((R,R)-IQNB), a muscarinic receptor antagonist, has been labeled with 123I and 125I for use in in vitro and in vivo studies in animals and humans. We have prepared fluoroalkyl analogs of QNB, which are amenable to labeling with 18F, for potential imaging applications with positron emission tomography. The enantiomers of (fluoroalkyl)benzilic acids were prepared via an enantioselective Grignard addition reaction. Subsequent coupling of the enantiomeric (fluoroalkyl)benzilic acid with a selected enantiomer of quinuclidinol provides fluorinated analogs of QNB with known stereochemistry at each of the stereogenic centers. These compounds exhibit different affinities for the muscarinic receptor tissue subtypes in vitro. (R,R)-4-(Fluoromethyl)-QNB, and (R,R)-IQNB, and (R,R)-4-(fluoroethyl)-QNB exhibit selectivity for the M1 subtype, and (R,S)-4-(fluoromethyl)-QNB exhibits selectivity for the M2 subtype.
Assuntos
Flúor/química , Quinuclidinil Benzilato/síntese química , Animais , Cristalografia por Raios X , Cobaias , Estrutura Molecular , Quinuclidinil Benzilato/análogos & derivados , Quinuclidinil Benzilato/farmacologia , EstereoisomerismoRESUMO
Enantioselectivity of acylations of (+/-)-cytallene (1b), (+/-)-N4-acetylcytallene (11a), (+/-)-N4-benzoylcytallene (11b), and (+/-)-N4-(9-fluorenylmethoxycarbonyl)cytallene (11c) using vinyl butyrate or acetate catalyzed by lipases in organic solvents was investigated. Reactions with 1b, 11a, and adenallene (1a) did not display a high enantioselectivity but all resulted in a predominant acylation of the (-)-enantiomers. Application of the Lowe-Brewster rule led to a tentative assignment of the R-configuration to all acylated products. Studies of the time course of acylation of (+/-)-N4-benzoylcytallene (11b) in chloroform, tetrahydrofuran (THF), tetrahydropyran (THP), tetrahydrothiophene (THT), and dioxane with lipase PS30 and/or AK showed that the reaction in THF catalyzed by lipase AK was the most promising for resolution of 11b. Indeed, a large-scale acylation afforded, after separation and deprotection of intermediates 3e and 10d, (+)- and (-)-cytallene (3c and 2b) in high yield and enantioselectivity. Acylation of 11c in THF led also to formation of 3c and 2b in high enantioselectivity. Single crystal X-ray diffraction established the S-configuration of (+)-cytallene (3c), thus confirming the assignment made on the basis of Lowe-Brewster rule. An improved large-scale synthesis of (+/-)-cytallene (1b) is also described. The R-enantiomer 2b inhibited the replication of a primary human immunodeficiency virus (HIV-1) isolate in phytohemagglutinin-activated peripheral blood mononuclear cells (PHA-PBM) with IC50 0.4 and IC90 1.7 microM. (+/-)-Cytallene (1b) exhibited IC50 0.8 and IC90 3.4 microM. Both compounds completely suppressed replication of HIV-1 at 10 microM with no detectable cytotoxicity. The S-enantiomer (3c) was inactive.
Assuntos
Antivirais/farmacologia , Citosina/análogos & derivados , HIV-1/efeitos dos fármacos , Lipase/metabolismo , Acilação , Antivirais/síntese química , Antivirais/química , Catálise , Células Cultivadas , Cristalografia por Raios X , Citosina/síntese química , Citosina/química , Citosina/farmacologia , HIV-1/fisiologia , Humanos , Monócitos/virologia , Estereoisomerismo , Replicação Viral/efeitos dos fármacosRESUMO
[ReCl2(C26H22P2)2].C6H14 (I), M(r) = 1136.11, monoclinic, P2(1)/c, a = 11.426 (2), b = 13.078 (2), c = 17.323 (3) A, beta = 95.27 (1) degrees, V = 2577.7 (6) A3, Z = 2, Dx = 1.46 g cm-3, lambda(Mo K alpha) = 0.71073 A, mu = 26.5 cm-1, F(000) = 1150, T = 296 K, R = 0.034 for 3269 [Fo2 > 3 sigma(Fo2)] reflections. The Re atom in (I) is located on an inversion point, fixed at the origin. One Cl and a complete tertiary phosphine ligand (Ph2PCHCHPPh2) are situated around the Re atom so as to generate a pseudo-octahedral geometry with a P1--Re--P2 angle of 79.07 (5) degrees and P1--Re--P2' angle of 100.93 (5) degrees. The Re--Cl, Re--P1 and Re--P2 distances are 2.432 (2), 2.401 (2) and 2.398 (2) A, respectively. Bond distances and angles for the phenyl rings range from 1.34 (1) to 1.41 (1) A and 117.4 (6) to 122.0 (7) degrees with averages of 1.38 (1) A and 120.0 (1) degrees, respectively.
Assuntos
Compostos Organometálicos/química , Compostos Organofosforados/química , Cristalografia por Raios X , Ligação de Hidrogênio , Estrutura MolecularRESUMO
Synthesis of optically pure (-)- and (+)-adenallene 2 and 3 is described. Racemic adenallene (1a) was subjected to deamination with adenosine deaminase monitored by HPLC using a Chiralcel CA-1 column to give (-)-adenallene (2) and (+)-hypoxallene (4). The latter compound was converted to acetate 5. The reaction of 5 with trifluoromethanesulfonic anhydride and pyridine followed by ammonolysis furnished acetate 6 or (+)-adenallene (3) depending on the solvent used in the last step. Acetate 5 was smoothly transformed to the 6-chloro derivative 7, but an attempted ammonolysis led only to racemization and decomposition. Single crystal X-ray diffraction established the R-configuration of (-)-enantiomer 2. The latter forms a pseudosymmetric dimer in the lattice with the adenine moiety in an anti-like conformation. The torsional angles of the allenic bonds show departures from 90 degrees (91 and 97 degrees, respectively) and rotameric preference of the hydroxymethyl groups is different in both molecules of the dimer. The R-enantiomer 2 inhibited the replication and cytopathic effect of human immunodeficiency virus (HIV-1) in ATH8 cell culture with an IC50 of 5.8 microM, whereas the S-enantiomer 3 was less active (IC50 > 200 microM). The enantioselectivity of the anti-HIV effect is significantly lower than that of 2',3'-dideoxyadenosine. Kinetics of deamination of R- and S-enantiomers 2 and 3 catalyzed by adenosine deaminase gave the following parameters: Km values of S-form 3 and R-form 2 were 0.41 and 0.52 mM with Vmax being 530 and 18.5 mumol/min, respectively [corrected]. Again,, a much lower level of enantioselectivity of deamination was observed than that of D- and L-adenosine. These results indicate (i) different enantioselectivity of enantiomers 2 and 3 as HIV inhibitors and adenosine deaminase substrates and (ii) both R- and S-enantiomers 2 and 3 can function as nucleoside analogues with varied enantioselectivity for different enzymes or receptors.
Assuntos
Adenina/análogos & derivados , Antivirais/síntese química , HIV-1/efeitos dos fármacos , Adenina/síntese química , Adenina/química , Adenina/farmacologia , Adenosina Desaminase/metabolismo , Antivirais/química , Antivirais/farmacologia , Efeito Citopatogênico Viral/efeitos dos fármacos , Desaminação , Cinética , Modelos Moleculares , Conformação Molecular , Estereoisomerismo , Relação Estrutura-Atividade , Replicação Viral/efeitos dos fármacosRESUMO
Novel homologated 19-oxiranyl- and 9-thiiranylandrost-4-ene-3,17-diones (8a,b and 9a,b, respectively) have been synthesized. The configuration and conformation of compound 8a have been established by X-ray crystallographic analysis. All four compounds have been shown to be competitive inhibitors of human placental aromatase. The thiiranes were more potent inhibitors than the corresponding oxiranes, and the 2'S isomers (8b and 9b) were better inhibitors than the 2'R (8a and 9a) diastereomers in each series. Spectroscopic studies with purified human placental aromatase suggest that the oxiranyl oxygen and thiiranyl sulfur of 2'S compounds 8b and 9b coordinate to the enzyme's heme iron.
Assuntos
Androstenodiona/análogos & derivados , Androstenodiona/síntese química , Inibidores da Aromatase , Compostos de Epóxi/síntese química , Placenta/enzimologia , Androstenodiona/química , Androstenodiona/farmacologia , Compostos de Epóxi/química , Compostos de Epóxi/farmacologia , Feminino , Compostos Heterocíclicos/síntese química , Compostos Heterocíclicos/química , Compostos Heterocíclicos/farmacologia , Humanos , Indicadores e Reagentes , Cinética , Espectroscopia de Ressonância Magnética , Modelos Moleculares , Conformação Molecular , Estrutura Molecular , Gravidez , Espectrofotometria Infravermelho , Relação Estrutura-Atividade , Difração de Raios XRESUMO
C18H15OP.1/2H2O, Mr = 287.30, orthorhombic, Fdd2, a = 19.794 (18), b = 32.540 (12), c = 9.459 (6) A, V = 6092.5 (22) A3, Z = 16, Dx = 1.253 g cm-3, lambda(Mo K alpha) = 0.71073 A, mu = 1.76 cm-1, F(000) = 2416, T = 294 K, R = 0.037 for 1677 reflections with I greater than 3 sigma(I). The water O atoms sit on a crystallographic twofold axis and are linked by hydrogen bonds to two of the oxide molecules with O...OW distances of 2.856 (3) A. Considerable conformational flexibility is conferred on the molecule by the long P--C bonds (around 1.8 A each).
Assuntos
Compostos Organofosforados , Fenômenos Químicos , Físico-Química , Análise de Fourier , Conformação Molecular , Estrutura Molecular , Difração de Raios XRESUMO
A detailed description of the two observed solution conformations of the pentapeptide lactone fragment of actinomycin D is presented using the distance constraints obtained from two-dimensional nuclear Overhauser enhancement spectra in combination with minimum energy calculations. Low energy conformational states that are compatible with the experimental distances are found for each of the two conformers. For one conformer, an all trans peptide bond conformer, is found with no intramolecular hydrogen bonds. For the other conformer, the D-Val-Pro and Pro-Sar peptide bonds were cis; this solution conformation is the same as that found in both the crystal structure of the pentapeptide lactone as well as of the native actinomycin D itself. These results are discussed in terms of the combined influence of conformation and the effects of mutual intramolecular association of the pentapeptide lactone moieties in native actinomycin D on its cytotoxic activity.
Assuntos
Dactinomicina/análise , Lactonas/análise , Conformação Proteica , Simulação por Computador , Espectroscopia de Ressonância Magnética , Modelos Moleculares , SoluçõesRESUMO
C22H34O4, Mr = 362.51, monoclinic, P2(1), a = 7.371 (1), b = 10.571 (1), c = 13.538 (2) A, beta = 90.36 (1) degree, V = 1054.84 A3, Z = 2, Dx = 1.141 Mg m-3, lambda(Cu K alpha) = 1.5418 A, mu = 0.577 mm-1, F(000) = 396, T = 296 K, R = 0.057 for 1550 unique reflections with I greater than or equal to sigma(I). The crystal conformation of the 12-membered ring in the title compound and its absolute configuration (1R) have been determined. The ring adopts a conformation with a necessarily approximate twofold axis in the crystal but it appears to be highly flexible.
Assuntos
Terpenos , Fenômenos Químicos , Físico-Química , Cristalização , Conformação Molecular , Estrutura Molecular , Terpenos/síntese química , Difração de Raios XRESUMO
2',3'-Dideoxyadenosine, C10H13N5O2, Mr = 235.24, orthorhombic, P2(1)2(1)2(1), a = 7.7404(4), b = 9.9843(9), c = 14.0842(10) A, V = 1088.46 A3, Z = 4, Dx = 1.435 Mg m-3, lambda(CuK alpha) = 1.5418 A, mu = 0.8326 mm-1, F(000) = 496, T = 296 K, final R = 0.032 for 1088 observed reflections. 2',3'-Dideoxycytidine, C9H13N3O3, Mr = 211.21, tetragonal, P4(1)2(1)2, a = 8.6802(4), c = 26.1386(14) A, V = 1969.44 A3, Z = 8, Dx = 1.424 Mg m-3, lambda(CuKa) = 1.5418 A, mu = 0.8701 mm-1, F(000) = 896, T = 296 K, final R = 0.050 for 1116 observed reflections. In both compounds the sugar rings have conformations intermediate between envelope and half chair but somewhat different pseudorotations. The relative orientations of the sugar and base are different in the two molecules with dideoxyadenosine being at the boundary of syn and anti and deoxycytidine being anti.
Assuntos
Didesoxiadenosina , Zalcitabina , Cristalografia , Ligação de Hidrogênio , Conformação Molecular , Estrutura Molecular , Difração de Raios XRESUMO
C21H31BrO4, Mr = 427.38, monoclinic, P2(1), a = 8.050 (1), b = 12.703 (3), c = 10.658 (1) A, beta = 102.56 (2) degrees, V = 1063.80 A3, Z = 2, Dx = 1.334 g cm-3, lambda(Cu K alpha) = 1.5418 A, mu = 28.09 cm-1, experimental temperature 296 K. F(000) = 448, R = 0.045 for 2015 unique reflections with I greater than or equal to sigma(I). The conformation of the 11-membered ring in this compound and its absolute configuration (1R) have been determined.
Assuntos
Canfanos , Canfanos/síntese química , Fenômenos Químicos , Química , Físico-Química , Cristalização , Cristalografia , Conformação Molecular , Estrutura MolecularRESUMO
Dioxadrol exists in four isomeric forms. alpha-(+)-Dioxadrol (dexoxadrol) showed phencyclidine (PCP)-like activity in rhesus monkeys trained to discriminate s.c. administration of ketamine, but neither alpha-(-)-dioxadrol (levoxadrol) nor beta-(+/-)-dioxadrol showed such activity. In addition, response-contingent i.v. dexoxadrol maintained higher rates of responding than either levoxadrol or beta-dioxadrol in monkeys experienced with ketamine self-administration. The order of potency in displacing bound 1-[1-(2-thienyl)cyclohexyl]piperidine from binding sites in rat brain homogenates was dexoxadrol much greater than levoxadrol = beta-(+/-)-dioxadrol. Viewed in the context of previous studies with stereochemical probes of the PCP receptor, these results extend and confirm the supposition that dexoxadrol and levoxadrol are the stereochemical probes of choice in the study of effects mediated through PCP receptors. The absolute configuration of dexoxadrol was determined to be 4S, 6S by X-ray crystallography, thus defining the optimum chirality necessary for receptor binding and PCP-like activity in the dioxadrol series. Based on these and other considerations, receptor-active conformations of dexoxadrol and PCP are proposed.
Assuntos
Analgésicos/farmacologia , Dioxolanos/farmacologia , Dioxóis/farmacologia , Piperidinas/farmacologia , Receptores de Neurotransmissores/metabolismo , Animais , Encéfalo/metabolismo , Dioxolanos/administração & dosagem , Dioxolanos/metabolismo , Discriminação Psicológica/efeitos dos fármacos , Isomerismo , Ketamina/farmacologia , Cinética , Macaca mulatta , Modelos Moleculares , Conformação Molecular , Fenciclidina/metabolismo , Piperidinas/administração & dosagem , Piperidinas/metabolismo , Receptores de Neurotransmissores/efeitos dos fármacos , Receptores da Fenciclidina , Autoadministração , Estereoisomerismo , Relação Estrutura-AtividadeRESUMO
IR, UV-visible spectroscopy, circular dichroism, 1H and 13C NMR studies, high resolution electron impact, field desorption, and fast atom bombardment mass spectral studies are reported for fredericamycin A (NSC-305263), a novel antitumor antibiotic of acid-base indicator type produced by Streptomyces griseus (FCRC-48). The spectral data are correlated with the structure obtained by X-ray crystallography as (E,E)-6',7'-dihydro-4,9,9'-trihydroxy-6-methoxy-3'-(1,3-pentadienyl++ +)-spiro- [2H-benz[f]indene-2,8'-[8H]-cyclopent-[g]-isoquinoline]-1,1', 3,5,8(2'H)-pentone. The novel spiro ring antibiotic exhibits unusual 1H and 13C NMR spectroscopic and chemical behavior, not previously observed in other antibiotic structures.
Assuntos
Antibióticos Antineoplásicos , Streptomyces griseus/metabolismo , Acetilação , Fenômenos Químicos , Química , Dicroísmo Circular , Cristalização , Isoquinolinas , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Metilação , Conformação Molecular , Espectrofotometria Infravermelho , Espectrofotometria Ultravioleta , Compostos de EspiroRESUMO
9-Methylene- and 9-ethylidene-5-(m-methoxyphenyl)-2-methylmorphans (1, 2) and refluxing 48% HBr have given rearrangement products 3 and 4, respectively. The structure of 4 [4a-ethyl-2,4a,5,6,7,7a-hexahydro-4-(3-hydroxyphenyl)-1-methyl-1H-1- pyrindine] was determined by X-ray crystallography and that of 3 [1,4a-dimethyl-2,4a,5,6,7,7a-hexahydro-4-(3-hydroxyphenyl)-1-methyl-1H- pyrindine] follows from analogy and NMR data. Compounds 3 and 4 are opioid antagonists of about the potency of nalorphine in the tail-flick vs. morphine assay and precipitate a complete abstinence syndrome in morphine-dependent monkeys. Both are nearly devoid of antinociceptive activity and they have about 0.025 times the affinity of nalorphine for the mu opioid receptor.
Assuntos
Alcaloides/farmacologia , Antagonistas de Entorpecentes , Piridinas , Alcaloides/metabolismo , Analgesia , Animais , Fenômenos Químicos , Química , Macaca mulatta , Masculino , Camundongos , Morfina/antagonistas & inibidores , Nalorfina/metabolismo , Nalorfina/farmacologia , Naloxona/farmacologia , Ratos , Ratos Endogâmicos , Receptores Opioides/metabolismo , Receptores Opioides mu , Sódio/farmacologia , Difração de Raios XRESUMO
The first enantiomeric pair of irreversible opioid ligands [(+)- and (-)-4] were synthesized in greater than 99.6% optical purity as determined by HPLC analysis of diastereoisomeric derivatives of the intermediate 3-methyl-N-phenyl-4-piperidinamine enantiomers. Single-crystal X-ray analysis of the (R,R)-L-(+)-tartaric acid salt of (-)-9 revealed the absolute configuration to be 3S,4R. The absolute configuration of (-)-3 [cis-(-)-3-methylfentanyl] and (-)-4 derived from (-)-9 is thus 3S,4R and that of (+)-3 and (+)-4 is 3R,4S. The (+) enantiomer of 4 (SUPERFIT) was shown to be highly potent and specific for acylation of delta opioid receptors (to the exclusion of mu) in rat brain membranes like its achiral prototype FIT and was about 10 times as potent as the latter in this assay. The (+)-4 was about 5 times as potent as FIT in acylation of delta receptors in NG108-15 neuroblastoma X glioma hybrid cells and about 50 times as potent as its enantiomer. Both FIT and (+)-4 behaved as partial agonists in inhibition of delta receptor coupled adenylate cyclase in NG108-15 membranes and (+)-4 was 5-10 times more potent than FIT and about 100 times more potent than its enantiomer in this assay. Dibromination of amine 12, catalytic exchange of bromine with tritium gas, and reaction of the labeled amine with thiophosgene afforded [3H]-(+)-4 with a specific activity of 13 Ci/mmol. Previous experiments indicated (+)-4 acylates the same 58 000-dalton glycoprotein previously shown to be acylated by FIT but with less nonspecific labeling. In view of the high potency and specificity of (+)-4 and the availability of its enantiomer, it seems likely that these compounds will prove to be valuable tools for study of the opioid receptor complex.
Assuntos
Fentanila/análogos & derivados , Isotiocianatos , Receptores Opioides/metabolismo , Acilação , Inibidores de Adenilil Ciclases , Analgésicos/farmacologia , Animais , Encéfalo/metabolismo , Encefalina Metionina/análogos & derivados , Encefalina Metionina/metabolismo , Fentanila/síntese química , Fentanila/farmacologia , Técnicas In Vitro , Ligantes , Camundongos , Conformação Molecular , Ratos , Receptores Opioides/análise , Receptores Opioides delta , Receptores Opioides mu , Estereoisomerismo , Trítio , Difração de Raios XRESUMO
The synthesis of racemic (3 alpha,6a alpha,11a beta)-1,3,4,5,6,11a- hexahydro-2-methyl-2H-3,6a-methanobenzofuro[2,3-c]azocin -10-ol (2d) is described. The route used acid-catalyzed ring closure of enamine 5 to yield the unsaturated phenylmorphan 6. Conversion of 6 to oxide-bridged 2d was accomplished in a multistep fashion that utilized the introduction of a bromine atom, followed by O-demethylation of the phenolic methyl ethers and base-catalyzed intramolecular phenoxide displacement of the bromine. Compound (+/-)-2d represents an oxide-bridged derivative of the potent 5-(m-hydroxyphenyl)morphan class of opioid analgesics 1. Unlike the 5-(m-hydroxyphenyl)morphans that have a freely rotating phenyl group, 2d has the phenyl ring conformationally restricted at an angle of 49 degrees relative to atoms 1, 3, 11a, and 12 of 2d. The low binding of (+/-)-2d to rat brain homogenate receptor preparations [IC50 = 1000 nM] may indicate that the phenyl angle of 49 degrees is not suitable for binding to opioid receptors.
Assuntos
Azocinas/síntese química , Benzofuranos/síntese química , Receptores Opioides/metabolismo , Animais , Modelos Moleculares , Ratos , Difração de Raios XRESUMO
Spin probes of deacetylcholchicine (1), 4-(hydroxymethyl)colchicine (2), and colchifoline (3) have been synthesized to study the binding site for colchicine on tubulin. Acylation of 1-3 with (+/-)-2,2,5,5-tetramethyl-1-pyrrolidinyloxy-3-carboxylic acid (4) afforded diastereomeric mixtures of the esters 5-8 and the amides 9 and 10. Pure diastereomers of 3 were synthesized with 4a and 4b, which inhibited the binding of colchicine by 60%. In the presence of calf brain microtubular protein, the colchifoline spin labels underwent reduction of the nitroxide group, which precluded their use to study the topography of the colchicine binding site.