[Glutamate dehydrogenase activity in the pancreatic tissue in acute experimental pancreatitis and under the action of sodium thiosulphate]. / Aktivnost' glutamatdegidrogenazy v tkani podzheludochnoi zhelezy pri ostrom éksperimental'nom pankreatite i pod vliianiem tiosul'fata natriia.

It has been established that the development of acute pancreatitis is accompanied by the reduced activity of glutamate dehydrogenase in the mitochondrial fraction of pancreas, pronounced in the focus of tissue necrosis and less expressed in the reactive inflammation focus. Besides this in the pancreas redistribution of enzyme, activity in the subcellular organelles takes place and enzyme activity emerges in the cytosol and further--in the blood and peritoneum liquid. Sodium thiosulfate has a marked correlation effect.

[The reactivity of postsynaptic alpha 1- and alpha 2-adrenoreceptors and arterial pressure in acute pancreatitis]. / Reaktivnost' postsinapticheskikh al'fa 1- i al'fa 2-adrenoretseptorov i arterial'noe davlenie pri ostrom pankreatite.

Experiments were conducted on pithed rats with acute pancreatitis and on falsely operated on animals (ether-laparotomy stress) to study the reactivity of vascular alpha 1- and alpha 2-adrenoreceptors and its role in changes in arterial pressure. Selective change in the reactivity of alpha 2-adrenoceptors alone was revealed in both groups. Stable reduction of vascular alpha 2-adrenergic reactivity (by 57-58%) and arterial pressure was recorded in animals with acute pancreatitis. In falsely operated on animals, in contrast, the values of these indices exceeded the control level. In animals with a functioning central nervous system inhibition of alpha 2-adrenergic reactivity correlated with change in arterial pressure only in the initial (3-4 hours) hemorrhagic phase of acute pancreatitis.

[A morphometric method of determining the volumetric density of structural components of tissue]. / Morfometricheskii sposob opredeleniia ob''emnoi plotnosti strukturnykh komponentov tkani.

Methods of determination in the histological sections of the volumetric fraction of the tissue components, characterized by low content (less than 1%), uneven distribution and wide size variation are described. The measuring of the volumetric part of such a structure is carried out by two stages: in the first stage the true area of the whole section is estimated under the low magnification; in the second stage the same is made for the true area of the structure investigated. Then the volumetric density of the component in question is determined by the ratio of the area of investigated structure and that of the whole section. By this method it is identified that the normal volumetric part of the insular apparatus of the rat pancreas is on the average 0.46% in the duodenal part and 0.57% in the spleen part.

[Diagnostic importance of determining leucine aminotransferase activity in acute pancreatitis]. / Diagnosticheskoe znachenie opredeleniia aktivnosti leitsin-aminotransferazy pri ostrom pankreatite.

It has been experimentally and clinically established that the determination of leucine-aminotransferase activity in the blood serum and abdominal exudate may serve as a marker for the early determination of pancreonecrosis and edematous (serous) pancreatitis.

[Ultrastructural changes in the acinar cells of the pancreas after exposure to cold]. / Ul'trastrukturnye izmeneniia atsinarnykh kletok podzheludochnoi zhelezy pod vozdeistviem kholoda.

Electron microscopy of acinar cell (AC) of the pancreas cooled to -30 degrees C with subsequent thawing has disclosed the destruction of intracellular membranes, damage to the cell organelles followed by diffusion of the zymogen granule contents to the hyaloplasm and interstice. These changes corresponded to the phase of paranecrosis (cryogenic shock of the cell), whereas the AC lost its ability to autophagocytosis. Irreversible cryodestruction provoked complete cell necrosis during 3-6 hours. The less cold-affected cells were subsequently destroyed because of additional injurious action of enzymatic cytolysis and circulatory hypoxia.

[Effect of prostaglandins E2 and F2 on mast cell serotonin content in experimental pancreatitis]. / Vliianie prostaglandinov E2 i F2 na soderzhanie serotonina v tuchnykh kletkakh pri éksperimental'nom pankreatite.

Rats with experimental pancreatitis manifest a lowering of the serotonin content in mast cells of the mesentery and connective tissue. Injection of prostaglandin E2 first causes a diminution and then an increase in the serotonin content of mast cells. Prostaglandin F2 alpha produces a lowering of the serotonin content of mast cells.

[Impairment of glycerophosphate and glycerol turnover in myocardium under conditions of experimental pancreatitis]. / Narushenie obmena glitserofosfata i glitserina v miokarde pri éksperimental nom pankreatite.

Initial steps of the experimental pancreatitis were characterized by an increase in glycerophosphate accumulation as well as in activity of glycerokinase, glycerophosphate dehydrogenase and glycerol dehydrogenase; content of free glycerol and dihydroacetone phosphate was also increased. Within the subsequent periods the patterns studied exhibited normalization except of glycerokinase and glycerophosphate dehydrogenase activity of which was distinctly decreased as compared with the controls.

[Effect of sodium thiosulfate on the pancreas in experimental pancreatitis]. / Vliianie tiosul'fata natriia na podzheludochnoi zhelezu pri éksperimental'noi pankreatite.

Histological and histochemical study of the pancreas of albino rats and experimental pancreatitis showed the use of sodium thiosulfate to considerably inhibit the progress of necrotic changes and circulatory disturbance. The preparation prevented recidivation of pancreatitis and inhibited sclerotic changes in the gland. Sodium thiosulfate stimulated the regenerative process including regenerative hypertrophy and expressed epimorphosis.

[Phospholipids in albino rat blood and liver at various stages in the development of experimental pancreatitis]. / Fosfolipidy krovi i pecheni belykh krys na razlichnykh étapakh razvitiia éksperimental'nogo pankreatita

In experimental pancreatitis in rats there was revealed a marked reduction, particularly on the 3rd-7th day of the disease, of neutral phospholipids in the blood plasma and formed elements. With the development of pancreatitis no polyglycerophospholipids (an important fraction of acid phospholipids participating in the formation of the oxidative enzymatic systems) were revealed in the liver. The described phenomena are regarded as significant pathogenetic factors leading to the development of fatty infiltration of the liver in pancreatitis.