Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes.

The DNA sequence of a fragment of 21731 bp (nucleotides 87408 to 109138) located on the left arm of chromosome VII from Saccharomyces cerevisiae S288C has been determined using a random cloning strategy followed by an oligonucleotide-directed sequencing. This fragment contains eight complete genes previously sequenced (CLG1, SKI8, VAM7, YPT32, MIG2, SIP2, SPT16 and CHC1), the 5' part of POX1 and two other complete unidentified open reading frames of more than 100 amino acids.

[The potentials of computed axial tomography (CAT) in the diagnosis of carcinoma of the cervix uteri]. / Vuzmozhnosti na kompiuturnata aksialna tomografiia (KAT) pri diagnoza na kartsinom na matochnata shiika.

14 women with diagnosis Ca colli uteri Ib stage were investigated. CAT (computer axial tomography) was carried out before and after surgery (Wertheim). CAT gave a picture of the tumor lesion as well as of the regional lymph nodes. This method proved fast, efficient and non invasive for the correct diagnosis of Ca colli uteri and surgery effect.

Glucose utilization in relation to insulin secretion in NZO and C57Bl mouse islets.

Further studies of insulin secretion from isolated pancreatic islets of the NZO strain of obese hyperglycemic mouse have shown markedly impaired insulin secretory responses to D-glucose in islets from fasted or fed mice. NZO islets at a low glucose concentration (3.3 mM) showed a significant insulin secretory response to 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), a phosphodiesterase inhibitor, but in the presence of this agent showed no significant additional response to increased glucose concentration. This contrasted with the situation in islets from an arbitrarily chosen control strain of mouse (C57Bl) which showed a small or insignificant response to 0.5 mM IBMX at a low glucose concentration, but a greatly enhanced response to glucose in the presence of IBMX. In contrast to the relative refractoriness of the NZO islets to glucose, they showed a large response to D,L-glyceraldehyde, at least equal to that found in the control islets. Glucose utilization was studied by measuring the conversion of D-[5-3H]glucose to [3H]H2O. In islets from both fasted and fed NZO mice, glucose utilization, when calculated on the basis of islet DNA content, was markedly reduced at high glucose concentrations compared to that in islets from the control strain. It is concluded that the relative unresponsiveness of NZO islets to glucose is associated with, and perhaps due to, a decreased rate of glucose utilization. The preserved responsiveness to glyceraldehyde suggests that the reduced glucose utilization may be due to a partial metabolic block before the triose phosphate step in the islet glycolytic pathway.

[Effect of combined hormonal oral contraceptives on several body systems]. / Deistvie na kombinirani khormonalni oralni kontratseptivni na niakoi sistemi na organizma

PIP: Literature on the biological effects of various combined hormonal contraceptives is briefly reviewed. Laparatomy findings in women who used hormonal contraceptives include decrease in the size of ovaries, fibrous changes in the ovary, atrophy of the endometrium, and changes in the luteal phase of the menstrual cycle. Epidemiologic data indicate increased incidence of thromboembolism in women using hormonal contraceptive agents. Prolonged use of hormonal contraceptives is associated with development of breast fibroadenomas, cellular atypia, proliferation of ductal epithelium and, in some instances, development of galactorrhea. Few studies report potential association between breast cancer and the use of hormonal contraceptives.^ieng