Effect of dermatan sulfate on the indentation and tensile properties of articular cartilage.

OBJECTIVE: This paper examines the hypothesis that the dermatan sulfate (DS) chain on decorin is a load carrying element in cartilage and that its damage or removal will alter the material properties. METHODS: To test this hypothesis, indentation and tensile testing of cartilage from bovine patella were performed before and after digestion with chondroitinase B (cB). Removal of significant amounts of DS by cB digestion was verified by Western blot analysis of proteoglycans extracted from whole and sectioned specimens. Specimens (control and treated) were subjected to a series of step-hold displacements. Elastic modulus during the step rise (rapid modulus) and at equilibrium (equilibrium modulus), and the relaxation function during each step was measured for test (cB and buffer) and control (buffer alone) conditions. RESULTS: cB had no effect on any of the viscoelastic mechanical properties measured, either in indentation or tension. CONCLUSION: Removing or damaging approximately 50% of the DS had no effect on the mechanical properties, strongly suggesting that DS either carries very low load or no load.

Evaluation of fracture toughness of cartilage by micropenetration.

Failure properties of cartilage are important in injury repair and disease, but few methods exist for measuring these properties, especially in small animals. To meet this need, a new indentation/penetration method for measuring fracture toughness of cartilage is proposed. During indentation, a conical tip is displaced into the surface of the cartilage, causing first a non-penetrating indentation, and then a penetration into the tissue. The method assumes that tissue penetration occurs during periods of "rapid work", which are identified from a curve of work rate vs. time. Total penetration depth is determined by summing the displacement during these periods. Fracture work is the work that occurs during "rapid work", or penetration, and fracture toughness defined as the fracture work divided by one-half the penetrated surface area of the indenting tip. The method was validated by indentation testing of bovine cartilage. Penetrating indentations with a conical tip were performed in bovine patellar cartilage and depth of penetration and fracture toughness predicted. For comparison with the indentation data, depth of penetration was measured in histological sections. These measurements agreed well with the predicted depth. Predicted fracture toughness also agreed with values measured via a macroscopic test. This newly described method has promise as a general method for measuring fracture toughness in cartilage, particularly in small animals, since penetrating tips with small tip radius can be manufactured and penetration may be accomplished in cartilage of minimal thickness.

A composites theory predicts the dependence of stiffness of cartilage culture tissues on collagen volume fraction.

The tensile stiffness of tissue grown from chondrocyte culture was both measured experimentally and predicted using a composites model theory relating tissue microstructure to macroscopic material stiffness. The tissue was altered by several treatment protocols to provide a wide range of collagen fibril volume fraction (0.015-0.15). The rate of change of tissue modulus with change in collagen volume fraction predicted by the theory was within 14% of the slope of the linear fit through the experimental data, without the use of fitting parameters for the theoretical value of the slope. Use of the model to simulate cytokine mediated tissue digestion suggests that the action of IL-1beta and retinoic acid is mainly removal of proteoglycans and some removal of collagen. The model also indicates that the matrix and collagen remaining in the tissue has the same elastic properties as the untreated tissue, and is not damaged due to the alteration. Young's modulus of the collagen fibrils is predicted to be 120 MPa, a value in the range of previous studies. This value is dependent mainly on the matrix modulus and collagen fibril volume fraction and not on Poisson's ratio of either matrix or fibril. Poisson's ratio of the tissue depends primarily on the Poisson's ratio of the matrix.