Long-Term Alterations in Pulmonary V˙O2 and Muscle Deoxygenation On-Kinetics During Heavy-Intensity Exercise in Competitive Youth Cyclists: A Cohort Study.

PURPOSE: The aim of this investigation was to assess alterations of pulmonary oxygen uptake (V˙O2) and muscle deoxygenation on-kinetics during heavy-intensity cycling in youth cyclists over a period of 15 months. METHODS: Eleven cyclists (initial age, 14.3 [1.6] y; peak V˙O2, 62.2 [4.5] mL·min-1·kg-1) visited the laboratory twice on 3 occasions within 15 months. Participants performed an incremental ramp exercise test and a constant workrate test within the heavy-intensity domain during the first visit and second visit, respectively. Subsequently, parameter estimates of the V˙O2 and muscle deoxygenation on-kinetics were determined with mono-exponential models. RESULTS: The V˙O2 phase II time constant decreased from occasion 1 (34 [4] s) to occasion 2 (30 [4] s, P = .005) and 3 (28 [4] s, P = .010). However, no significant alteration was observed between occasions 2 and 3 (P = .565). The V˙O2 slow component amplitude either expressed in absolute values (ie, L·min-1) or relative to end exercise V˙O2 (ie, %) showed no significant changes throughout the study (P = .972 and .996). Furthermore, the muscle deoxygenation on-kinetic mean response time showed no significant changes throughout the study (18 [8], 18 [3], and 16 [5] s for occasions 1, 2, and 3, respectively; P = .279). CONCLUSION: These results indicate proportional enhancements of local muscle oxygen distribution and utilization, which both contributed to the speeding of the V˙O2 on-kinetics herein.

Physiological Characteristics of Competitive Male Junior Cyclists Transitioning to the Under-23 Level: A Retrospective Comparative Study.

PURPOSE: The purpose of the current investigation was to retrospectively assess possible differences in physiological performance characteristics between junior cyclists signing a contract with an under-23 (U23) development team versus those failing to sign such a contract. METHODS: Twenty-five male junior cyclists (age: 18.1 [0.7] y, stature: 181.9 [6.0] cm, body mass: 69.1 [7.9] kg, peak oxygen uptake: 71.3 [6.2] mL·min-1·kg-1) were assigned to this investigation. Between September and October of the last year in the junior category, each cyclist performed a ramp incremental exercise test to determine certain physiological performance characteristics. Subsequently, participants were divided in 2 groups: (1) those signing a contract with a U23 development team (JUNIORU23) and (2) those failing to sign such a contract (JUNIORNON-U23). Unpaired t tests were used to assess possible between-groups differences in physiological performance characteristics. The level of statistical significance was set at P < .05 two tailed. RESULTS: No significant between-groups differences in submaximal (ie, gas exchange threshold, respiratory compensation point) and maximal physiological performance characteristics (ie, peak work rate, peak oxygen uptake) expressed in absolute values (ie, L·min-1, W) were observed (P > .05). However, significant between-groups differences were observed when physiological performance characteristics were expressed relative to the cyclists' body weights (P < .05). CONCLUSIONS: The current investigation showed that junior cyclists stepping up to a U23 development team might be retrospectively differentiated from junior cyclists not stepping up based on certain physiological performance characteristics, which might inform practitioners and/or federations working with young cyclists during the long-term athletic development process.

Alterations in aerobic fitness and muscle deoxygenation during ramp incremental exercise in trained youth cyclists: a ~3-year longitudinal study.

Alterations of aerobic fitness and muscle deoxygenation during a ramp incremental exercise test (GXT) were assessed on two occasions within a time-frame of 2.9 ± 0.1y in competitive youth cyclists. Nine cyclists (age, 14.5 ± 1.1y; peak oxygen uptake (V˙O2peak), 62.6 ± 4.2 mL.min-1.kg-1) participated in this investigation. V˙O2peak, the gas exchange threshold (GET) and the respiratory compensation point (RCP), as well as the muscle deoxygenation response pattern were determined during a GXT using open circuit spirometry and near-infrared spectroscopy, respectively. T-tests and Pearson's correlations were used to assess effects of time on the dependent variables and relationships between changes of parameter estimates of aerobic fitness and the muscle deoxygenation response, respectively. Workrate and metabolic rate at GET (33 ± 20 and 42 ± 23%) and RCP (36 ± 20 and 40 ± 22%), and V˙O2peak (30 ± 18%) significantly increased throughout the study (P < 0.05). The muscle deoxygenation response showed a significant rightward shift from occasion one to two (P < 0.05). Alterations in the workrate/metabolic rate at RCP, and V˙O2peak, were correlated with alterations of the muscle deoxygenation response (R = 0.71-0.89, P < 0.05). Together, this is thought to indicate a superior muscle perfusion within the tissue of interrogation at the same metabolic rate on occasion two vs. one, which partially contributed to the improved aerobic fitness in the cyclists herein.

Longitudinal alterations of pulmonary V . O2 on-kinetics during moderate-intensity exercise in competitive youth cyclists are related to alterations in the balance between microvascular O2 distribution and muscular O2 utilization.

Purpose: The main purpose of the current study was to investigate the dynamic adjustment of pulmonary oxygen uptake ( V . O2) in response to moderate-intensity cycling on three occasions within 15 months in competitive youth cyclists. Furthermore, the muscle Δdeoxy[heme] on-kinetics and the Δdeoxy[heme]-to- V . O2 ratio were modeled to examine possible mechanistic basis regulating pulmonary V . O2 on-kinetics. Methods: Eleven cyclists (initial age, 14.3 ± 1.6 y; peak V . O2, 62.2 ± 4.5 mL.min-1.kg-1) with a training history of 2-5 years and a training volume of ~10 h per week participated in this investigation. V . O2 and Δdeoxy[heme] responses during workrate-transitions to moderate-intensity cycling were measured with breath-by-breath spirometry and near-infrared spectroscopy, respectively, and subsequently modeled with mono-exponential models to derive parameter estimates. Additionally, a normalized Δdeoxy[heme]-to- V . O2 ratio was calculated for each participant. One-way repeated-measures ANOVA was used to assess effects of time on the dependent variables of the responses. Results: The V . O2 time constant remained unchanged between the first (~24 s) and second visit (~22 s, P > 0.05), whereas it was significantly improved through the third visit (~13 s, P = 0.006-0.013). No significant effects of time were revealed for the parameter estimates of the Δdeoxy[heme] response (P > 0.05). A significant Δdeoxy[heme]-to- V . O2 ratio "overshoot" was evident on the first (1.09 ± 0.10, P = 0.006) and second (1.05 ± 0.09, P = 0.047), though not the third (0.97 ± 0.10, P > 0.05), occasion. These "overshoots" showed strong positive relationships with the V . O2 time constant during the first (r = 0.66, P = 0.028) and second visit (r = 0.76, P = 0.007). Further, strong positive relationships have been observed between the individual changes of the fundamental phase τp and the Δdeoxy[heme]-to- V . O2 ratio "overshoot" from occasion one to two (r = 0.70, P = 0.017), and two to three (r = 0.74, P = 0.009). Conclusion: This suggests that improvements in muscle oxygen provision and utilization capacity both occurred, and each may have contributed to enhancing the dynamic adjustment of the oxidative "machinery" in competitive youth cyclists. Furthermore, it indicates a strong link between an oxygen maldistribution within the tissue of interrogation and the V . O2 time constant.

Elite versus non-elite cyclist - Stepping up to the international/elite ranks from U23 cycling.

This study investigated the physiological, performance and training characteristics of U23 cyclists and assessed the requirements of stepping up to the elite/international ranks. Twenty highly trained U23 cyclists (age, 22.1 ± 0.8 years; body mass, 69.1 ± 6.8 kg; VO2max, 76.1 ± 3.9 ml·kg-1·min-1) participated in this study. The cyclists were a posteriori divided into two groups based on whether or not they stepped up to elite/international level cycling (U23ELITE vs. U23NON-ELITE). Physiological, performance and training and racing characteristics were determined and compared between groups. U23ELITE demonstrated higher absolute peak power output (p = .016), 2 min (p = .026) 5 min (p = .042) and 12 min (p ≤ .001) power output as well as higher absolute critical power (p = .002). Further, U23ELITE recorded more accumulated hours (p ≤ .001), covered distance (p ≤ .001), climbing metres (p ≤ .001), total sessions (p ≤ .001), total work (p ≤ .001) and scored more UCI points (p ≤ .001). These findings indicate that U23ELITE substantially differed from U23NON-ELITE regarding physiological, performance and training and racing characteristics derived from laboratory and field. These variables should be considered by practitioners supporting young cyclists throughout their development towards the elite/international ranks.

Effects of Flat and Uphill Cycling on the Power-duration Relationship.

The purpose of this study was to investigate the effects of flat and uphill cycling on critical power and the work available above critical power. Thirteen well-trained endurance athletes performed three prediction trials of 10-, 4- and 1-min in both flat (0.6%) and uphill (9.8%) cycling conditions on two separate days. Critical power and the work available above critical power were estimated using various mathematical models. The best individual fit was used for further statistical analyses. Paired t-tests and Bland-Altman plots with 95% limits of agreement were applied to compare power output and parameter estimates between cycling conditions. Power output during the 10- and 4-min prediction trial and power output at critical power were not significantly affected by test conditions (all at p>0.05), but the limits of agreement between flat and uphill cycling power output and critical power estimates are too large to consider both conditions as equivalent. However, power output during the 1-min prediction trial and the work available above critical power were significantly higher during uphill compared to flat cycling (p<0.05). The results of this investigation indicate that gradient affects cycling time-trial performance, power output at critical power, and the amount of work available above critical power.

Climbing Performance in U23 and Professional Cyclists during a Multi-stage Race.

The aim of this study was to analyze climbing performance across two editions of a professional multistage race, and assess the influence of climb category, prior workload, and intensity measures on climbing performance in U23 and professional cyclists. Nine U23 cyclists (age 20.8±0.9 years) and 8 professional cyclists (28.1±3.2 years) participated in this study. Data were divided into four types: overall race performance, climb category, climbing performance metrics (power output, ascent velocity, speed), and workload and intensity measures. Differences in performance metrics and workload and intensity measures between groups were investigated. Power output, ascent velocity, speed were higher in professionals than U23 cyclists for Cat 1 and Cat 2 (p≤0.001-0.016). Workload and intensity measures (Worktotal, Worktotal∙km-1, Elevationgain, eTRIMP and eTRIMP∙km-1) were higher in U23 compared to professionals (p=0.002-0.014). Climbing performance metrics were significantly predicted by prior workload and intensity measures for Cat 1 and 2 (R2=0.27-0.89, p≤0.001-0.030) but not Cat 3. These findings reveal that climbing performance in professional road cycling is influenced by climb categorization as well as prior workload and intensity measures. Combined, these findings suggest that Cat 1 and 2 climbing performance could be predicted from workload and intensity measures.

Aerobic and Anaerobic Power Distribution During Cross-Country Mountain Bike Racing.

PURPOSE: To determine aerobic and anaerobic demands of mountain bike cross-country racing. METHODS: Twelve elite cyclists (7 males; V˙O2max = 73.8 [2.6] mL·min-1·kg-1, maximal aerobic power [MAP] = 370 [26] W, 5.7 [0.4] W·kg-1, and 5 females; V˙O2max = 67.3 [2.9] mL·min-1·kg-1, MAP = 261 [17] W, 5.0 [0.1] W·kg-1) participated over 4 seasons at several (119) international and national races and performed laboratory tests regularly to assess their aerobic and anaerobic performance. Power output, heart rate, and cadence were recorded throughout the races. RESULTS: The mean race time was 79 (12) minutes performed at a mean power output of 3.8 (0.4) W·kg-1; 70% (7%) MAP (3.9 [0.4] W·kg-1 and 3.6 [0.4] W·kg-1 for males and females, respectively) with a cadence of 64 (5) rev·min-1 (including nonpedaling periods). Time spent in intensity zones 1 to 4 (below MAP) were 28% (4%), 18% (8%), 12% (2%), and 13% (3%), respectively; 30% (9%) was spent in zone 5 (above MAP). The number of efforts above MAP was 334 (84), which had a mean duration of 4.3 (1.1) seconds, separated by 10.9 (3) seconds with a mean power output of 7.3 (0.6) W·kg-1 (135% [9%] MAP). CONCLUSIONS: These findings highlight the importance of the anaerobic energy system and the interaction between anaerobic and aerobic energy systems. Therefore, the ability to perform numerous efforts above MAP and a high aerobic capacity are essential to be competitive in mountain bike cross-country.

Power Profiling, Workload Characteristics, and Race Performance of U23 and Professional Cyclists During the Multistage Race Tour of the Alps.

PURPOSE: The aim of this study was to compare the power profile, internal and external workloads, and racing performance between U23 and professional cyclists and between varying rider types across 2 editions of a professional multistage race. METHODS: Nine U23 cyclists from a Union Cycliste Internationale "Continental Team" (age 20.8 [0.9] y; body mass 71.2 [6.3] kg) and 8 professional cyclists (28.1 [3.2] y; 63.0 [4.6] kg) participated in this study. Rider types were defined as all-rounders, general classification (GC) riders, and domestiques. Data were collected during 2 editions of a 5-day professional multistage race and split into the following 4 categories: power profile, external and internal workloads, and race performance. RESULTS: The professional group, including domestiques and GC riders, recorded higher relative power profile values after certain amounts of total work (1000-3000 kJ) than the U23 group or all-rounders (P ≤ .001-.049). No significant differences were found for external workload measures between U23 and professional cyclists, nor among rider types. Internal workloads were higher in U23 cyclists and all-rounders (P ≤ .001-.043) compared with professionals, domestiques, and GC riders, respectively. The power profile significantly predicted percentage general classification and Union Cycliste Internationale points (R2 = .90-.99), whereas external and internal workloads did not. CONCLUSION: These findings reveal that the power profile represents a practical tool to discriminate between professionals and U23 cyclists as well as rider types. The power profile after 1000 to 3000 kJ of total work could be used by practitioners to evaluate the readiness of U23 cyclists to move into the professional ranks, as well as differentiate between rider types.

Training Characteristics and Power Profile of Professional U23 Cyclists throughout a Competitive Season.

BACKGROUND: The purpose of this study was to investigate differences in the power profile derived from training and racing, the training characteristics across a competitive season and the relationships between training and power profile in U23 professional cyclists. METHODS: Thirty male U23 professional cyclists (age, 20.0 ± 1.0 years; weight, 68.9 ± 6.9 kg; V˙O2max, 73.7 ± 2.5 mL·kg-1·min-1) participated in this study. The cycling season was split into pre-, early-, mid- and late-season periods. Power data 2, 5, 12 min mean maximum power (MMP), critical power (CP) and training characteristics (Hours, Total Work, eTRIMP, Work·h-1, eTRIMP·h-1, TimeVT2) were recorded for each period. Power profiles derived exclusively from either training or racing data and training characteristics were compared between periods. The relationships between the changes in training characteristics and changes in the power profile were also investigated. RESULTS: The absolute and relative power profiles were higher during racing than training at all periods (p ≤ 0.001-0.020). Training characteristics were significantly different between periods, with the lowest values in pre-season followed by late-season (p ≤ 0.001-0.040). Changes in the power profile between early- and mid-season significantly correlated with the changes in training characteristics (p < 0.05, r = -0.59 to 0.45). CONCLUSION: These findings reveal that a higher power profile was recorded during racing than training. In addition, training characteristics were lowest in pre-season followed by late-season. Changes in training characteristics correlated with changes in the power profile in early- and mid-season, but not in late-season. Practitioners should consider the influence of racing on the derived power profile and adequately balance training programs throughout a competitive season.

Validity and Reliability of the Garmin Vector Power Meter in Laboratory and Field Cycling.

To assess the validity and reliability of the Garmin Vector against the SRM power meter, 6 cyclists completed 3 continuous trials at power outputs from 100-300 W at 50-90 rev·min-1 and a 5-min time trial in laboratory and field conditions. In field conditions only, a 30-s sprint was performed. Data were compared with paired samples t-tests, with the 95% limits of agreement (LoA) and the typical error. Reliability was calculated as the coefficient of variation (CV). There was no significant difference between the devices in power output in laboratory (p=0.245) and field conditions (p=0.312). 1-s peak power was significantly different between the devices (p=0.043). The LoA were ~1.0±5.0 W and ~0.5±0.5 rev·min-1 in both conditions. The LoA during the 30-s sprint was 6.3±38.9 W and for 1-s peak power it was 18.8±17.1 W. The typical error for power output was 2.9%, while during sprint cycling it was 7.4% for 30-s and 2.7% for 1-s peak power. For cadence, the typical error was below 1.0%. The mean CVs were ~1.0% and ~3.0% for the SRM and Garmin, respectively. These findings suggest, that the Garmin Vector is a valid alternative for training. However, during sprint cycling there is lower agreement with the SRM power meter. Both devices provide good reliability (CV<3.0%).

Gross efficiency during flat and uphill cycling in field conditions.

PURPOSE: While a number of studies have investigated gross efficiency (GE) in laboratory conditions, few studies have analyzed it in field conditions. Therefore, the aim of this study was to analyze the effect of gradient and cadence on GE in field conditions. METHODS: Thirteen trained cyclists (mean ± SD age 23.3 ± 4.1 y, stature 177.0 ± 5.5 cm, body mass 69.0 ± 7.2 kg, maximal oxygen uptake [VO2max] 68.4 ± 5.1 mL · min-1 ·kg-1) completed an incremental graded exercise test to determine ventilatory threshold (VT) and 4 field trials of 6 min duration at 90% of VT on flat (1.1%) and uphill terrain (5.1%) with 2 different cadences (60 and 90 rpm). VO2 was measured with a portable gas analyzer and power output was controlled with a mobile power crank that was mounted on a 26-in mountain bike. RESULTS: GE was significantly affected by cadence (20.6% ± 1.7% vs 18.1% ± 1.3% at 60 and 90 rpm, respectively; P < .001) and terrain (20.0% ± 1.5% vs 18.7% ± 1.7% at flat and uphill cycling, respectively; P = .029). The end-exercise VO2 was 2536 ± 352 and 2594 ± 329 mL/min for flat and uphill cycling, respectively (P = .489). There was a significant difference in end-exercise VO2 between 60 (2352 ± 193 mL/min) and 90 rpm (2778 ± 431 mL/min) (P < .001). CONCLUSION: These findings support previous laboratory-based studies demonstrating reductions in GE with increasing cadence and gradient that might be attributed to changes in muscle-activity pattern.

Microbial degradation of chlorobenzene under oxygen-limited conditions leads to accumulation of 3-chlorocatechol.

Five bacterial strains (Acidovorax facilis B517, Cellulomonas turbata B529, Pseudomonas veronii B547, Pseudomonas veronii B549, and Paenibacillus polymyxa B550) isolated on chlorobenzene as the sole source of carbon and energy were screened for the accumulation of the putative metabolic intermediate 3-chlorocatechol during growth on chlorobenzene under oxygen-limited conditions in the presence and absence of nitrate (1 mM). 3-Chlorocatechol accumulated in the growth media of all five strains, but accumulation was significantly less in cultures of A. facilis B517 compared to the other four strains. The presence of nitrate did not influence the biological conversion pattern. However, biologically produced nitrite reacted with 3-chlorocatechol chemically, a reaction that masked the accumulation of 3-chlorocatechol. For P. veronii B549, a clear relationship between the presence of 3-chlorocatechol in the medium and low oxygen concentrations was demonstrated. The assumption is made that accumulation of 3-chlorocatechol is due to the low enzymatic turnover of the 3-chlorocatechol cleaving enzyme, catechol-1,2-dioxygenase, at low oxygen concentrations.