PRELI, the human homologue of the avian px19, is expressed by germinal center B lymphocytes.

We report the identification of a human cDNA encoding a 25 kDa protein of relevant evolutionary and lymphoid interest (PRELI). PRELI was cloned by screening a B lymphocyte-specific cDNA library with a probe generated by mRNA differential display. PRELI amino acid sequence is 85% similar to the avian px19 protein, expressed within the blood islands and in the liver during avian embryo development. PRELI and px19 contain tandem repeats (A/TAEKAK) of the late embryogenesis abundant (LEA) motif, characteristic of a group of survival molecules and originally thought to be present only in plant proteins. Interestingly, PRELI expression is high in the fetal liver, a major site for B cell lymphopoiesis, while the mRNA levels in other fetal tissues such as the brain, lung, and kidney are comparatively low. At the adult stage, PRELI expression is drastically reduced in the liver but exhibits high mRNA levels in the spleen, brain, lung and kidney tissues, suggesting that PRELI expression may be important for the development of vital and immunocompetent organs. Moreover, PRELI is also highly expressed in the adult lymph nodes and peripheral blood leukocytes, further stressing that at the adult stage, PRELI expression may be important during secondary immune responses. Consistent with this hypothesis, the expression of PRELI is predominant within germinal centers (GC), a stage in which B lymphocytes are under a stressful selection pressure. Taken together these data: (i) strongly support the notion that the conserved LEA motif represents a phylogenetic link between plants and animals, (ii) reveal a novel molecule whose expression may play a role in the maturation of distinct human tissues, and (iii) suggest that PRELI expression may be important for GC B lymphocytes.

Irreversible silicone oil adhesion to silicone intraocular lenses. A clinicopathologic analysis.

PURPOSE: To report a newly defined complication of foldable intraocular lenses (IOLs), namely silicone oil-silicone IOL interaction. This is a complication not generally seen by the implanting cataract surgeon but, rather, at a later stage in a patient's postoperative course, by a vitreoretinal surgeon. METHODS: Three clinical case histories, including two explanted silicone IOLs, were submitted for analysis. The submitted silicone lenses were photographed under water, and the nature of the silicone oil coating was documented. RESULTS: In each instance, the silicone coating was manifest as a thick coating with droplet formation on the lens surface that was tenaciously adherent and could not be dislodged by instruments or injection of viscoelastics. CONCLUSION: The use of silicone IOLs in patients with current vitreoretinal disease or those who are at high risk for future vitreoretinal disease that may require silicone oil as part of the therapy should be reconsidered. The authors recommend that information regarding the existence and significance of this complication be printed on all silicone oil and silicone IOL packages and inserts (if not as a warning, at least as an informative comment regarding the existence of this condition). This is a rare but clinically significant complication that will affect the occasional patient treated with both of these modalities.

Centration and fixation of silicone intraocular lenses: clinicopathological findings in human autopsy eyes.

PURPOSE: To analyze centration and fixation of three-piece and one-piece silicone intraocular lenses (IOLs) in human autopsy eyes. SETTING: Center for Intraocular Lens Research, Medical University of South Carolina, Charleston. METHODS: Thirty eyes among those submitted to the center between 1986 and 1994 were evaluated. The globes were sectioned at the equatorial plane and photographed from a posterior view. Gross examination was performed to determine IOL type, fixation, centration, and additional pathology. The results were compared with those of a control group of autopsy eyes implanted with one-piece and three-piece poly(methyl methacrylate) (PMMA) IOLs. The control group matched the silicone group in patient age, fixation site, and duration of implantation. RESULTS: Twenty-three eyes (79.3%) had three-piece silicone IOLs and 7 eyes (20.7%), one-piece plate IOLs. Average patient age in the three-piece group was 77.3 years +/- 6.7 (SD) and in the one-piece group, 74.3 +/- 3.8 years. The 30 IOLs were symmetrically fixated in the bag. Average decentration of the three-piece and one-piece IOLs did not differ significantly, 0.37 +/- 0.31 mm and 0.26 +/- 0.13 mm, respectively (P = .37). There was also no significant difference in decentration between the silicone groups and the PMMA groups (P = .93). CONCLUSION: Centration and fixation of silicone IOLs were equivalent to those of standard PMMA IOLs.

Analysis of 100 explanted one-piece and three-piece silicone intraocular lenses.

PURPOSE: The authors analyzed incidence and types of complications seen after implantation of silicone posterior chamber intraocular lenses (PC IOLs). These data were compared with those obtained in previous studies with standard polymethylmethacrylate IOLs. METHODS: Data from the laboratory database were collected on 100 explanted silicone IOLs submitted to the Center for Intraocular Lens Research from 1986 to 1994. RESULTS: Of the 100 IOLs, 63 were three-piece designs with polypropylene loops and 37 were one-piece plate lenses. The two most important reasons for explantation were IOL decentration (42.0%) and inflammation (27.7%). There was no significant difference for the reasons of explantations between one- and three piece IOLs (P > 0.1). Intraocular lenses that had been removed due to inflammatory reactions had a significantly longer implant duration (19.2 +/- 18.9 months) than lenses that were explanted because of decentration (9.19 +/- 12.1 months) (P = 0.018). CONCLUSIONS: The results presented here showed that there is no substantial difference between these IOLs and polymethylmethacrylate lenses in terms of reasons for explantation. The authors' data suggest that clinical outcome is not as dependent on the type of lens selected as it is on the quality of surgery. The key to good results is the use of "capsular surgery," namely techniques that ensure secure in-the-bag IOL fixation.

Pathways for metabolism of ketoaldonic acids in an Erwinia sp.

The pathways involved in the metabolism of ketoaldonic acids by Erwinia sp. strain ATCC 39140 have been investigated by use of a combination of enzyme assays and isolation of bacterial mutants. The catabolism of 2,5-diketo-D-gluconate (2,5-DKG) to gluconate can proceed by two separate NAD(P)H-dependent pathways. The first pathway involves the direct reduction of 2,5-DKG to 5-keto-D-gluconate, which is then reduced to gluconate. The second pathway involves the consecutive reduction of 2,5-DKG to 2-keto-L-gulonate and L-idonic acid, which is then oxidized to 5-keto-D-gluconate, which is then reduced to gluconate. Gluconate, which can also be produced by the NAD(P)H-dependent reduction of 2-keto-D-gluconate, is phosphorylated to 6-phosphogluconate and further metabolized through the pentose phosphate pathway. No evidence was found for the existence of the Entner-Doudoroff pathway in this strain.